Lettuce (Lactuca sativa L.) were transformed using microparticle bombardment with two different genes, alpha-glucuronidase (GUS) gene and Chinese cabbage Glutathione Reductase (GR) gene. The adventitious shoots of cotyledonary explant from 4-day-old seedlings were formed (46.7%) in MS basal media supplemented with 5.0 μm IAA and 1.0 μm 2ip. When 1100 psi helium pressure, 9 target distance, and coating with tungsten 10 microparticles were used and explants were treated with osmoticum-conditioning medium (0.6M sorbitol/mannitol), 4 h prior to and 16 h after bombardment, it was identified by GUS assay that these conditions were the most efficient for transformation of foreign genes into cotyledon tissue of lettuce with particle bombardment. PCR confirmed that the band observed in the transgenic plants were originated from T-DNA tranfer with strong hybridization. The genomic Southern analysis showed that the 1.5-kbp fragment was hybridized with radiolabeled 1.5-kbp GR probe. To know whether the expression of the GR gene can be stably maintained in the next generation, when T2 selfing seeds that were obtained from the transformed mother plants were sowed on MS medium supplemented with 200 μm kanamycin, 70% of seedlings were revealed resistance to kanamycin.
J.D. Chung, B.J. Lee, H.S. Lee and C.K. Kim
E.T. Pippin, E.W. Bush, D.J. Lee and R.E. Strahan
Weeping lovegrass (Eragrostis curvula) is commonly used in native areas bordering golf courses in the Southeastern United States. These areas do not receive significant levels of maintenance, thus weed encroachment is a problem that can negatively impact the functional and aesthetic values of the golf course. The objectives of this study is to determine which selective postemergent herbicides labeled for use on golf courses can remove weeds from Weeping Lovegrass and to determine the level of phytotoxicity. Herbicides included monosodium methane arsenate (MSMA 6.0) applied at 3.0 lb/acre a.i., sulfosulfuron (Certainty) at 0.047 lb/acre a.i., metribuzin (Sencor 75 DF) at 0.5 lb/acre a.i., and imazaquin (Image 70 DG) at a rate of 0.5 lb/acre a.i.. Treatments were applied on July 20, 2004 to 9.6 × 9.6 plots arranged in a randomized complete block design (RCBD) using Teejet 8005 nozzles at 40 psi calibrated to deliver 40 ga/acre. Plots were monitored daily and data was collected 0, 7, 14, 21, 28, 35, and 42 DAT. Sulfosulfuron and MSMA provided the highest level of weed control 35 DAT. Metribuzin and imazaquin provided limited weed suppression compared to the control. Initial phytotoxic damage to the Lovegrass was observed in all herbicide treatments. The highest level of phytotoxic damage was observed in the MSMA and Metribuzin treatments; however there was no apparent damage at 42 DAT. Herbicide applications of sulfosulfuron and MSMA are effective in reducing weed populations with acceptable levels of phytotoxicity to the Lovegrass.
O. Gulsen, R.C. Shearman, K.P. Vogel, D.J. Lee, P.S. Baenziger, T.M. Heng-Moss and H. Budak
Buffalograss [Buchloe dactyloides (Nutt.) Engelm.] has the potential for increased use as a turfgrass species due to its low maintenance and water conservation characteristics. This study was conducted to estimate diversity and relationships among naturally occurring buffalograss genotypes based on the nuclear genome, using sequence-related amplified polymorphism (SRAP) markers. The 56 genotypes studied represented five ploidy levels collected from diverse geographic locations in the North American Great Plains. In addition, blue grama [Bouteloua gracilis (H.B.K.) Lag. Ex Steud.] and perennial ryegrass (Lolium perenne L.) were included as outgroups. Twenty-five combinations of forward and reverse primers were used. Ninety-five intensively amplified markers were scored and used to infer diversity and relationships among the genotypes. All buffalograss genotypes were discriminated from each other with similarity values ranging from 0.70 to 0.95. Principal component analysis (PCA) suggested that the 56 genotypes could be reduced to 50 due to high similarity levels among some of the genotypes. The distance between buffalograsses, blue grama, and perennial ryegrass were consistent with current taxonomical distances. This research indicates that SRAP markers can be used to estimate genetic diversity and relationships among naturally occurring buffalograss genotypes.
Youngjae Oh, Jason D. Zurn, Nahla Bassil, Patrick P. Edger, Steven J. Knapp, Vance M. Whitaker and Seonghee Lee
The availability of strawberry (Fragaria ×ananassa) genomic resources has increased dramatically in recent years. Some of these resources are readily applicable to strawberry breeding programs for use in DNA-informed breeding. Information about these tests and how to interpret them is dispersed through numerous manuscripts or in the laboratories that use them routinely. To assist breeders in identifying tests available to their breeding program and in implementing them in their program, a compendium of strawberry DNA tests was created. This compendium is available for download from the Genome Database for Rosaceae (https://www.rosaceae.org/organism/Fragaria/x-ananassa?pane=resource-4). This resource will be updated continually as old tests are modified and new tests are created.
Elsa S. Sánchez, Thomas M. Butzler, Lee J. Stivers, Timothy E. Elkner, Steven M. Bogash, R. Eric Oesterling and Michael D. Orzolek
Butternut, acorn, and buttercup/kabocha winter squash (Cucurbita sp.) cultivars were evaluated in a conventional system in central, southeastern, and southwestern Pennsylvania in 2010–11. Results from individual locations were used to create statewide recommendations, which are also relevant for the mid-Atlantic U.S. region. Additionally, butternut and acorn cultivars were evaluated in an organic system in central Pennsylvania. In a conventional system, butternut cultivars JWS6823, Betternut 401, Quantum, and Metro are recommended based on equal or higher marketable yield than the standard Waltham Butternut. Acorn squash cultivars that performed equally to or better than the standard, Tay Belle, were Table Star, Harlequin, and Autumn Delight. In the kabocha/buttercup category, ‘Sweet Mama’ and ‘Red Kuri’ had marketable yields not different from the standard ‘Sunshine’ in central and southeastern Pennsylvania. In the organic system, butternut cultivars JWS6823, Betternut 401, and Metro all had marketable yields not different from the standard Waltham Butternut. For acorn cultivars, Celebration yield did not differ from the standard Table Queen.
Elsa S. Sánchez, Thomas M. Butzler, Steven M. Bogash, Timothy E. Elkner, R. Eric Oesterling, Michael D. Orzolek and Lee J. Stivers
Sixteen cultivars of green bell peppers (Capsicum annuum) were evaluated on the basis of yield in three locations across Pennsylvania during the growing seasons of 2008–09. Cultivars were evaluated in comparison with the cultivar Paladin. In central Pennsylvania, all the cultivars trialed had marketable yields (based on weight) not different than ‘Paladin’ except ‘Lynx’, ‘Socrates’, and ‘Escape’. In terms of fruit number, all cultivars were not different than ‘Paladin’ except ‘Socrates’. For large-sized fruit, all the cultivars trialed are recommended. In southeastern Pennsylvania, all the cultivars trialed except SP-05–47 had marketable yields not different than ‘Paladin’. For large-sized fruit, ‘Revolution’ outperformed all other cultivars, including ‘Paladin’. In southwestern Pennsylvania, all the cultivars trialed except Lynx and SP-05–47 produced comparable marketable yields to ‘Paladin’. None of the cultivars evaluated, including Paladin, consistently outperformed Revolution in terms of large fruit. Statewide, all the cultivars, except Lynx and SP-05–47, are recommended on the basis of marketable yields. For growers looking for large-sized fruit to meet market demand the cultivar Revolution is recommended over ‘Paladin’.
Desalegn D. Serba, Osman Gulsen, Bekele G. Abeyo, Keenan L. Amundsen, Donald J. Lee, P. Stephen Baenziger, Tiffany M. Heng-Moss, Kent M. Eskridge and Robert C. Shearman
Hybridization and selection has been one of the methods used to generate turfgrass cultivars in buffalograss improvement. Three half-sib populations were developed by crossing three buffalograss female genotypes, NE 3296, NE 2768, and NE 2769, with NE 2871, a male genotype, to 1) investigate the pattern of genetic variability generated for turfgrass characteristics through hybridization; 2) assess the effect of parental change on the level of genetic variability generated in a buffalograss diploid population; and 3) predict the performance of a progeny generated from two heterozygous parents for turfgrass performance. The four parents and 20 random F1 progeny selected from each population were established in 2006 at the John Seaton Anderson Turfgrass Research Facility located near Mead, NE. A randomized complete block design (RCBD) was used with the progeny nested in the crosses. A visual rating scale of 1–9 was used to evaluate the population. Mean population lateral spread, genetic color, density, and turfgrass quality from early summer to fall ranged from 3.5 to 4.5, 7.1 to 7.9, 6.9 to 8.1, and 5.2 and 6.8, respectively. There were significant differences among the crosses and the parents for all the traits studied except quality in June and August. The progeny nested within crosses differed for turfgrass genetic color and quality. Best linear unbiased prediction (BLUP) indicated a high improvement potential for turfgrass lateral spread and spring density in NE 2768 × NE 2871 and for turfgrass genetic color in NE 3296 × NE 2871. From these findings, it can be concluded that hybridization breeding is a worthwhile approach for generating and identifying transgressive segregants for specific buffalograss traits.