Sw-5 is a locus introgressed from Lycopersicon peruvianum to some L. esculentum lines conferring dominant resistance to TSWV. Restriction fragment length polymorphism (RFLP) analyses positions Sw-5 to the long arm of chromosome 9 in the sub-telomeric region between CT71 and CT220. RFLP analyses suggest the introgressed region begins distal to CT71, includes CT220, and may extend to the telomere. Randomly amplified polymorphic DNA (RAPD) analyses with >700 random 10-mer primers identified a single 2.2-kbp band with one primer (primer #72 GAGCACGGGA) that is tightly linked to Sw-5. However, we have also produced an equivalent 2.2-kbp band in analysis of other TSWV-susceptible tomato breeding lines. Thus, this band likely derives from L. esculentum DNA very near to Sw-5 and the introgressed region. Additional analyses have recently detected a potential co-dominant RAPD polymorphism linked to Sw-5.
M.R. Stevens, P.D. Griffiths, J.W. Scott, D.K. Heiny, and D.D Rhoads
Peter C. Andersen, Fred M. Rhoads, Steven M. Olson, and Kristen D. Hill
Carbon and nitrogen budgets were determined for `Colonial' (spring) and `Equinox' (fall) tomato (Lycopersicon esculentum Mill.) plants grown on raised beds with black polyethylene mulch and supplied with preplant-N at 0, 67, 134, 202, or 269 kg·ha–1. For both spring and fall experiments, we quantified the partitioning of dry matter, N, and C, and determined marketable and total yield. In the spring study, the concentration of N in leaves, stems, and in total plants increased linearly with level of N fertilization, whereas a quadratic relationship described the amount of N contained in the fruit (maximum with 202 kg·ha–1). Quadratic relationships occurred between rate of fertilization and leaf weight, stem weight, total plant weight, marketable yield, and total yield in the spring study, with maximum values at 134 or 202 kg·ha–1 rates of N fertilization. In the fall crop, fewer significant relationships occurred between dependent variables and rate of N fertilization, and coefficients of determination tended to be much lower than in the spring study. The fraction of N in leaves, stems, and roots (fall study only) was influenced by N fertilization. Effects of N fertilization on the fraction of C partitioned to any plant part was either nonsignificant or significant at P = 0.05. Total yield was related to N fertilization in a quadratic manner, but marketable yield was significantly affected only in the spring study. In both studies, increasing the rate of N fertilization reduced the C: N linearly for all tissues. In all cases, the quantity of N partitioned to vegetative tissue was at least 65% of that partitioned to the fruit, and the quantity of C in the plant was at least 74% of that in the fruit. In conclusion, although N fertilization above 202 kg·ha–1 generally increased the concentration and total amount of N in vegetative tissues, it did not increase yield. Also, the highest rate of N fertilization (269 kg·ha–1) resulted in a much lower efficiency of applied N [defined as: (N plant + N fruit)/N applied], and a much higher level of residual soil nitrate-N.