A study was conducted to evaluate the possibility of producing and processing immature cowpea (Vigna unguiculata) green pods by using the same technology used for green beans (Phaseolus vulgaris). The cowpea cultivar Bettersnap developed for green pod production and the green bean cultivars Benton and OSU-5402 were produced under the same cultural conditions. `Bettersnap' yielded less than 0.5 ton/ha, while `Benton' and `OSU-5402' produced about 2.5 ton/ha in once-over simulated mechanical harvest. `Bettersnap' had long vines and dense foliage, which resulted in plants with more width and less erectness than `Benton', the predominant green bean cultivar. Uneven pod setting and long pods (23.8 cm) in `Bettersnap' constitute potential problem for mechanical harvest. Canned cowpea pods of sieves 2 and 3 had darker green color, smaller seeds, and higher shear value, fiber content, and sloughing than green bean pods. Our study indicates that there is a need to develop cultivars with high yield potential and concentrated pod setting adapted to mechanical harvest with pods containing less fiber and less tendency to sloughing.
A.R. Gonzalez, D.E. Smith, A. Mauromoustakos and M. Davis
N.J. George, D.L. Madhavi and M.A.L. Smith
Many plants can produce bioactive chemicals with medicinal or health benefits, which has stimulated a whole new research effort aimed at extracting & improving natural phytochemicals. Begonia is a rich source of biologically-active phytochemicals and an excellent donor for natural anthocyanin pigments. High levels of triterpene compounds and a host of potentially-useful flavonoids have been isolated from Begonia sp., which may account for its frequent use as a medicinal plant remedy in a diverse array of cultures worldwide. Deliberate shifting of the physical and chemical microenvironments can have a significant effect on anthocyanins and precursors produced in vitro. This realization offers the potential to thoroughly screen and study valuable phytochemicals from Begonia. Begonia genotypes from 3 species were screened to identify callus induction techniques. Contamination inherent in the vascular system of one genotype, along with spontaneous organogenesis, were found to be recurrent problems. These were partially alleviated by light and growth regulator treatments. Studies comparing callus and in vitro vegetative tissues as resources for phytochemical extraction are scheduled.
M.A.L. Smith, D. Seigler and F.E. Kandil
Polyphenolic compounds (particularly anthocyanins, proanthocyanidins, and other flavonoids) from some fruits and vegetables have significant and diverse impacts on human health preservation. While it's well recognized that some of the polyphenolics in foods we consume have a protective and proactive role against disease, very little has been known about how they accomplish this feat. A range of bioassays (in vitro and in laboratory animals) were adapted to examine compounds extracted from berry fruits, and separated into distinct fractions by vacuum chromatography. The proanthocyanidin class of compounds, as well as mixtures of proanthocyanidins and other flavonoids, were significantly bioactive against both the promotion and initiation stages of chemically-induced carcinogenesis. Potent antioxidant activity was not confined to particular fractions, but was present in several classes of compounds. Identification and characterization of the bioflavonoids is complicated both by apparent interactions between related compounds that occur together within horticultural fruits, and interferences from some substances (pectins and complex sugars) that depress observed response in bioactivity assays.
D.A. Smith, J.B. Fitzgerald and G.E. Meyer
Vitalization is a process whereby senescence is retarded and refrigerated storage can be extended. The process involves hyperhydration of plant materials with selected aqueous solution, thereby flooding interstitial spaces and vascular tissues. Microscopic examination revealed increased size of interstitial spaces and expansion and increased roundness to cells. No disruption of tissues was detected. Turgidity was measured with an Instron Universal Testing Machine equipped with a Kramer Shear Cell. Color was measured with a Minolta color difference meter. Leaves were evaluated for color and turgidity changes during storage. Vitalized leaves did not change significantly in color or turgidity during a 10-week storage period. Untreated leaves lost turgidity and yellowed in storage.
D.A. Smith, M.L. Metz and S.L. Cuppett
Dry edible beans (Phaseolis vulgaris) represent an inexpensive way to incorporate protein into the diet as a food ingredient, but beans contain unpleasant flavors and several anti-nutritional factors that limit their use without first processing with long heat treatments. `Great Northern' bean flour was processed using either static or specially designed dynamic (continuous) processing methods. The dynamic process treated flour slurries at temperatures up to 124°for 20 sec. The slurries were quick-frozen and freeze-dried after frozen storage periods of 0, 8, 24, 120, or 504 hr. The flours were analyzed for sensory properties, emulsifying activity, foaming properties, and trypsin inhibition. The heat treatments improved sensory attributes of the flour. The foam capacity and foam stability decreased in heat-treated flours. Trypsin inhibitor activity was at a minimum level immediately following thermal processing, but increased with time in frozen storage prior to drying. Minimal thermal processes cannot be relied upon to inactivate trypsin inhibitors.
Beth Ann A. Workmaster, Jiwan P. Palta and Jonathan D. Smith
In Wisconsin, the cranberry plant (Vaccinium macrocarpon Ait.) is protected from freezing temperatures by flooding and sprinkle irrigation. Due to the high value of the crop, growers typically overprotect by taking action at relatively warm temperatures. Our goal is to provide recommendations for improved frost protection strategies by studying seasonal hardiness changes in different parts of the cranberry plant (leaves, stems, buds, flowers, fruit). Stages of bud growth were defined and utilized in the hardiness determinations. Samples were collected from mid-April to mid-Oct. 1996 and cuttings were subjected to a series of freezing temperatures in a circulating glycol bath. Damage to plant parts was assessed by visual scoring and observation, ion leakage, and evaluation of the capability to regrow. The following results were obtained: 1) Overwintering structures, such as leaves, stems, and buds, can survive temperatures <–18°C in early spring, and then deacclimate to hardinesses between 0 and –2°C by late spring. 2) In the terminal bud floral meristems are much more sensitive to freeze–thaw stress than are the vegetative meristems. 3) Deacclimation of various plant parts occurred within 1 week, when minimum canopy temperatures were above 0°C, and when the most numerous bud stage collected stayed the same (bud swell). 4) Fruits >75% blush can survive temperatures of –5°C for short durations. By collecting environmental data from the same location we are attempting to relate plant development, frost hardiness, and canopy temperatures (heat units).
B. Castillo, D.L. Madhavi and M.A.L. Smith
Interaction between irradiance levels (5–40 mM–m–2–s–1) and iron chelate sources (FeNa2EDTA and FeNaDTPA) on the establishment, growth, and proliferation of shoot tips of Carica papaya were tested. Reduced irradiance level (5 mM–m–2–s–1) enhanced the establishment of shoot tips regardless of the source of iron chelate tested. At higher irradiance levels (30 and 40 mM–m–2–s–1), presence of FeNaDTPA in the medium enhanced establishment of shoot tips. Continuous or alternating light/dark (16/8 h) photoperiods at high irradiance levels had no effect on the establishment or growth of the culture. At higher irradiance levels, the cultures produced smaller leaves as compared to lower irradiance levels. Low irradiance and FeNa2EDTA was preferred during the proliferation stage.
Malcolm W. Smith, Jeremy D. Bright, Mark D. Hoult, Richard A. Renfree, Tony Maddern and Neil Coombes
Despite an abundance of polyembryonic genotypes and the need for rootstocks that improve scion yield and productivity, simultaneous field testing of a wide range of mango (Mangifera indica L.) genotypes as rootstocks has not previously been reported. In this experiment, we examined the growth and yield of ‘Kensington Pride’ on 64 mango genotypes of diverse origin during the first four seasons of fruit production to identify those worth longer-term assessment. We also recorded morphological characteristics of seedlings of 46 of these genotypes in an attempt to relate these measures to subsequent field performance. Tree canopy development on the most vigorous rootstocks was almost double that on the least vigorous. Growth rates differed by more than 160%. Cumulative marketable yield ranged from 36 kg/tree for the lowest yielding rootstock to 181 kg/tree for the most productive. Yield efficiency also differed markedly among the 64 rootstocks with the best treatment being 3.5 times more efficient than the poorest treatment. No relationship was found between yield efficiency and tree size, suggesting it is possible to select highly efficient rootstocks of differing vigor. Two genotypes (‘Brodie’ and ‘MYP’) stood out as providing high yield efficiency with small tree size. A further two genotypes (‘B’ and ‘Watertank’) were identified as offering high yield efficiency and large tree size and should provide high early yields at traditional tree spacing. Efforts to relate the morphology of different genotype seedlings to subsequent performance as a rootstock showed that nursery performance of mango seedlings is no indication of their likely behavior as a rootstock. The economic cost of poor yields and low yield efficiencies during the early years of commercial orchard production provide a rationale for culling many of the rootstock treatments in this experiment and concentrating future assessment on the top ≈20% of the 64 treatments. Of these, ‘MYP’, ‘B’, ‘Watertank’, ‘Manzano’, and ‘Pancho’ currently show the most promise.