Greenhouse studies were conducted at the Univ. of Florida to evaluate the effects of preemergence herbicides on St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] rooting. Metolachlor, atrazine, metolachlor + atrazine, isoxahen, pendimethalin, dithiopyr, and oxadiazon were applied to soil columns followed by placement of St. Augustinegrass sod on the treated soil. Root elongation and biomass were measured following application. Plants treated with dithiopyr and pendimethalin had no measurable root elongation and root biomass was severely (>70%) reduced at the study's conclusion (33 days). Root biomass was unaffected following isoxaben and oxadiazon treatments, but oxadiazon applied at 3.4 kg·ha-1 reduced root length by 50%. Atrazine at 2.2 kg·ha-1 and metolachlor + atrazine at 2.2 + 2.2 kg·ha-1, did not reduce root length in one study, while the remaining atrazine and metolachlor + atrazine treatments reduced cumulative root length and total root biomass 20% to 60%. Metolachlor at 2.2 kg·ha-1 reduced St. Augustinegrass root biomass by >70% in one of two studies. St. Augustinegrass root elongation rate was linear or quadratic in response to all treatments. However, the rate of root elongation was similar to the untreated control for plants treated with isoxaben or oxadiazon. Chemical names used: 6-chloro-N-ethyl-N'-(l-methylethyl)-1,3,5-triazine-2,4-diamine(atrazine);S,S-dimethyl2-(difluoromethyl)-4-(2-methylpropyl)-6-(t∼fluoromethyl)-3,5-pyridinecarbothioate (dithiopyr); N-[3-(1-ethyl-1-methylpropyl)-5-isoxazolyl]-2,6-dimethoxybenzamide (isoxaben); 2-chloro-N-(2-ethyl- 6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide (metolachlor); 3-[2,4-dichloro-5-(1-methylethoxy)phenyl]-5-(1,1-dimethylethyl)-1,3,4-oxadiazol-2-(3H)-one (oxadiazon); N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzenamine (pendimethalin).