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- Author or Editor: D. A. Williamson x
- HortScience x
Abstract
The cover depicts a cross-sectional photomicrograph of a root from peach treated with a soil application of paclobutrazol. The 14-μm section was taken about 2 mm from the root apex. The tissue was fixed and embedded by conventional methods similar to those described by Sass (8), then doublestained with safranin and fast green. Photomicrographs were taken at × 180 with Ektachrome film (Kodak) using a Leitz Dialux 20 research microscope equipped with an Orthomat automatic microscope camera.
Abstract
Seed of snap bean, Phaseolus vulgaris L. cv. Avalanche were separated into 3 length or 3 diameter groups and then each group separated into 3 classes based on aerodynamic properties. The grading procedure resulted in seed grades having large differences in physical characteristics, growth and yield responses. Yield response potential of snap bean was determined primarily by seed weight. A grading method utilizing size grading based on seed diameter followed by aspiration in a vertical air column was the most effective method of eliminating seed with low yield potential.
Abstract
Seed of 11 cultivars of snap bean, Phaseolus vulgaris L., were separated by aerodynamic properties in a vertical air column. Seed physical characteristics associated with air column separation were weight, density, volume, diameter, and length. The separation technique did not affect seed germination, seedling emergence, or plant survival at full expansion of the first trifoliate leaf growth stage. However, seed remaining in the air column after aspiration produced fewer weak plants and fewer plants with root rot at the first trifoliate leaf. These seed produced a greater plant stand, a greater pod weight per plant, a more uniform pod size distribution, and a greater yield at harvest than the seed removed. Yield from seed remaining after air column aspiration was 21% greater than from non-graded seed.
Increasing salinity of agricultural soils may ultimately limit the sustainability of food production in some areas of the world. Work from our laboratory and the labs of others demonstrates that mannitol, a six-carbon sugar alcohol, is important as a stress-related metabolite in some plants. Mannitol helps plants resist the damaging effects of stressful growth environments, such as drought, high soil salinity, and perhaps attack by microorganisms that cause plant diseases. In the long run, we hope to genetically engineer plants to produce and use mannitol for increased productivity and tolerance to environmental stresses. Basic information about how plants regulate those genes important to mannitol metabolism is of critical importance to this long-term goal. Our laboratory discovered an enzyme, mannitol dehydrogenase, that is the first critical biochemical step in mannitol use in vascular plants. Later, we cloned the gene for this enzyme. We discovered that hexose sugars “turn off” the expression of this gene. So, as long as adequate sugars are available for energy, maintenance, and growth, the production of the mannitolusing enzyme is repressed. After the sugars are gone, mannitol dehydrogenase is produced very rapidly, and this allows mannitol to be used metabolically. This type of gene regulation is ideally designed to help plants cells conserve mannitol as long as possible, which in turn allows the cells to retain stress tolerance as long as possible.
Mannitol, a six carbon sugar alcohol, is widely distributed in nature and is a major phloem-translocated photoassimilate in celery. II may also function as a compatible osmolyte providing stress tolerance. Until recently, little was known about the route of mannitol catabolism in sink tissues of higher plants. An enzyme. mannitol dehydrogenase. (MDH) that oxidizes mannitol to mannose utilizing NAD as the electron acceptor was discovered (Arch. Biochem. Biophys. 1991. 298:612-619) in “sink” tissues of celery and celeriac plants. The activity of the enzyme is inversely related to tissue mannitol concentration in various parts of celery plants suggesting a role for the enzyme in mannitol catabolism. In osmostressed celery plants, the activity of the enzyme in sink tissues decreases as mannitol accumulates.
Celery cells growing heterotrophically in suspension culture utilize either sucrose or mannitol as the sole carbon source and grow equally well on either carbohydrate. Mannitol-grown cells contain more MDI-I activity than sucrose-grown cells, and the activity of the enzyme is correlated with the rate of depletion of mannitol from the culture medium. Cells growing on mannitol contain an internal pool of mannitol but little sugar. Cells growing on sucrose contain internal sugar pools but no mannitol. Mannitol-grown cells are also more salt tolerant than cells grown on sucrose. Our laboratory is involved in studies of the physiological role of the mannitol oxidizing enzyme in regulating mannitol utilization and the role of the enzyme in regulating mannitol pool size during salt and osmostress in both celery plants and celery suspension cultures. Current studies on the molecular control of expression of the enzyme will be discussed.