The objective of these experiments was to determine if preemergence herbicides perform similarly across pine bark that was aged for varying lengths of time including 0, 4, 8, and 12 months after bark removal from harvested trees. Three preemergence herbicides were evaluated for three separate weed species, including 1) Cardamine flexuosa With. (bittercress) with isoxaben, 2) Digitaria sanguinalis (L.) Scop. (large crabgrass) with prodiamine, and 3) Oxalis stricta L. (woodsorrel) with dimethenamid-P. Leaching of herbicides through substrates was evaluated for prodiamine. Weed growth in the various substrates was variable, but few differences were detected in weed growth among the pine bark substrates evaluated. For isoxaben and prodiamine, weed control was similar among the pine bark substrates in most cases when label rates were applied. Although some differences were detected in prodiamine performance across different pine bark ages, a high level of control was achieved in all cases at rates well below manufacturer recommendations. Prodiamine leaching was minimal in all substrates. It would be recommended that growers test substrates for physical properties before use so that irrigation and other production inputs could be modified if needed. In most cases, growers should expect similar performance of preemergence herbicides regardless of pine bark substrate age.