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  • Author or Editor: Carole H. Saravitz x
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Carole H. Saravitz, Frank A. Blazich and Henry V. Amerson

Adventitious shoots developed on cotyledons of Virginia pine (Pinus virginiana Mill.) excised from seeds germinated for 3, 6, or 9 days and cultured on media containing 0.5 to 10 mg/liter benzyladenine (BA). Shoot regeneration was greatest (46 shoots per embryo) on cotyledons from seeds germinated for 6 days and placed on medium containing 10 mg/liter BA. Shoots were excised and elongated on medium lacking BA. Following elongation, shoots were placed on media containing 0 to 40 mg/liter indolebutyric acid (IBA) for 14 days followed by transfer to the same medium lacking auxin. Without IBA treatment, percent rooting was 3% and increased to 50% for concentrations of 5 to 40 mg/liter. Rooted shoots averaged 2.0 roots per shoot without auxin treatment, 3.3 roots when treated with 5 mg/liter IBA and root number increased linearly with increased IBA concentration up to 40 mg/liter (4.5 roots). Plant lets were transferred to growing medium and acclimated successfully to greenhouse conditions.

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Carole H. Saravitz, Frank A. Blazich and Henry V. Amerson

Adventitious shoots developed on cotyledons of Virginia pine (Pinus virginiana Mill.) excised from seeds germinated for 3, 6, or 9 days and cultured on media containing 0.5 to 10 mg/liter benzyladenine (BA). Shoot regeneration was greatest (46 shoots per embryo) on cotyledons from seeds germinated for 6 days and placed on medium containing 10 mg/liter BA. Shoots were excised and elongated on medium lacking BA. Following elongation, shoots were placed on media containing 0 to 40 mg/liter indolebutyric acid (IBA) for 14 days followed by transfer to the same medium lacking auxin. Without IBA treatment, percent rooting was 3% and increased to 50% for concentrations of 5 to 40 mg/liter. Rooted shoots averaged 2.0 roots per shoot without auxin treatment, 3.3 roots when treated with 5 mg/liter IBA and root number increased linearly with increased IBA concentration up to 40 mg/liter (4.5 roots). Plant lets were transferred to growing medium and acclimated successfully to greenhouse conditions.

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Carole H. Saravitz, Frank A. Blazich and Henry V. Amerson

Hypocotyls of Fraser fir (Abies fraseri (Pursh) Poir.) were excised from seeds germination 9 days and placed on bud induction medium containing 10 mg/liter benzyladenine (BA) and 0.01 mg/liter naphthaleneacetic acid (NAA) or medium without growth regulators. After 3 days on medium containing growth regulators, cell divisions were localized in epidermal and subepidermal layers of the hypocotyl while similar cell divisions were not observed in control-treated hypocotyls. Cell clusters consisting of two to five cells were present after 7 days in hypocotyls placed on bud induction medium. In control-treated hypocotyls, stomata continued to develop and cells within the cortex became vacuolated during the first 2 weeks in culture. All hypocotyls were transferred to secondary medium after 3 weeks. Cell clusters continued to enlarge into meristemoids in hypocotyls initially placed on bud induction medium. Gradually, meristemoids developed into buds and cataphylls were observed covering bud meristems.

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Carole H. Saravitz, Frank A. Blazich and Henry V. Amerson

Hypocotyl cuttings were prepared from Ii-week-old aseptically grown seedlings of Fraser fir [Abies fraseri (Pursh) Poir.] and cultured 18 days on media containing 0 to 40 mg IBA/liter followed by transfer to the same medium without auxin. Greatest rooting (66%) occurred after treatment with 20 mg IBA/liter, whereas the greatest number of roots per rooted cutting (7.4) was noted following treatment with 40 mg·liter-1. Chemical name used: 1H-indole-3-butyric acid (IBA).

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Carole H. Saravitz, Frank A. Blazich and Henry V. Amerson

Adventitious shoots developed on cotyledons of Virginia pine (Pinus virginiana Mill.) excised from seeds subjected to H2O2 treatment for 3, 6, or 9 days and cultured on media containing 0.5 to 10 mg BA/liter. Shoot regeneration was greatest (42 shoots per embryo) on cotyledons from seeds treated with H2O2 for 6 days and placed on medium containing BA at 10 mg·liter-1. Excised shoots elongated on medium lacking BA. Following elongation, shoots were placed on media containing IBA at 0 to 40 mg·liter-1 for 14 days followed by transfer to the same medium lacking auxin. Without IBA treatment, rooting was 3%, and increased to 50% for 5 to 40 mg·liter-1. Rooted shoots averaged 2.0 roots per shoot without auxin incorporation, 3.3 roots when treated with 5 mg IBA/liter, and the number of roots increased linearly with increased IBA concentration up to 40 mg·liter-1 (4.5 roots). Plantlets were transferred to growing medium and acclimated successfully to greenhouse conditions. Chemical names used: N- (phenylmethyl)-1 H- purine-6-amine (BA), 1 H- indole-3-butyric acid CBA).

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Carole H. Saravitz, Frank A. Blazich and Henry V. Amerson

Cotyledons and hypocotyls of Fraser fir [Abies fraseri (Pursh) Poir.] were excised from seeds treated with H2 O2 for 9 days and placed on bud induction medium containing 10 mg BA/liter and 0.01 mg NAA/liter or medium without growth regulators. Although adventitious buds did not develop, cotyledons exposed to growth regulators responded differently than cotyledons placed on medium lacking growth regulators. Cotyledons and hypocotyls responded similarly to growth regulators during the initial phase in culture, but cell divisions ceased in cotyledons, thus preventing meristemoid and subsequent bud development. After 3 days on medium containing growth regulators cell divisions were localized in epidermal and subjacent layers of hypocotyls, whereas similar cell divisions were' not observed in hypocotyls placed on medium without growth regulators. Cell clusters consisting of two to five cells (promeristemoids) were present after 7 days on hypocotyls placed on bud induction medium. In hypocotyls placed on medium without growth regulators, stomata continued to develop and cells within the cortex became vacuolated during the first 2 weeks in culture. All explants were transferred to secondary medium after 3 weeks. Cell clusters continued to enlarge into meristemoids on hypocotyls initially placed on bud induction medium. Gradually, meristemoids developed into buds and cataphylls were observed covering bud meristems. Chemical names used: N -(phenylmethyl)-1 H -purine-6-amine (BA), 1-naphthaleneacetic acid (NAA).