Sales of organic foods are one of the fastest growing segments of Washington state's food industry. In response to grower demand for information on organic and sustainable production practices, Washington State University (WSU) created an Extension Agricultural Systems position. This position has been instrumental in helping WSU gain the trust and recognition of organic growers. The position enabled WSU to demonstrate that it has a commitment to organic and sustainable research and extension activities. This paper describes the key activities of this position: 1) finding out research needs, 2) on-farm research approaches, 3) formation of regional research programs, and 4) creation of the WSU Food and Farm Connections Team. Grant funded on-farm research, interdisciplinary teams, and extension publications have been major emphases of the position.
Carol A. Miles
Improving sweet corn fertilizer-N efficiency promotes a more vigorous and healthy crop, rewards the grower with greater profits, and protects our water resources from nitrate-N pollution. Two areas of research that have the potential to improve the efficiency of fertilizer-N applications are the Minolta SPAD 502 chlorophyll meter and the presidedress soil N test. The SP meter is a rapid and nondestructive technique for assessing sweet corn leaf levels, and SP readings have been correlated to leaf N concentration. A presidedress soil N test measures the amount of soil N that will be available to the plant during the remainder of the growing season. SP meter readings combined with presidedress soil N analyses may be used to determine crop N needs and fertilizer-N sidedress application rates. Basing fertilizer-N sidedress application rates on actual crop N needs will reduce excess fertilizer-N applications and the resulting leaching of nitrates.
Carol A. Miles
Commercial edamame (Glycine max) varieties and advanced edamame breeding lines from the Asian Vegetable Research Development Center (AVRDC) were tested for adaptability to southwest Washington. Edamame, or green vegetable soybeans, are specialty varieties of soybeans that are eaten at the green stage as a vegetable. For the vegetable market, 25 beans must weigh at least 20 g. Experimental procedure was a randomized complete block design with four replications. Recommendations from AVRDC for plant spacing and fertilizer application and timing were followed. In 1995, 13 commercial varieties and 10 AVRDC breeding lines were tested in an on-farm location in Chehalis. At the same location in 1996, 10 of these commercial varieties were again tested along with an additional six commercial varieties. Also in 1996, 12 new AVRDC breeding lines were tested along with the single line that was selected in 1995. Both years, all commercial varieties were harvested more than 40 days later than their advertised days to maturity. Three commercial varieties, White Lion, Shironomai, and Butterbeans, were high-yielding in both years. In 1995, one AVRDC breeding line was selected in Chehalis, and in 1996 five additional AVRDC breeding lines were selected. Earliness is a key factor affecting suitability of commercial varieties and breeding lines to the Chehalis area. In this region, irrigation also appears essential for production of large beans for the vegtable market. Pod weight was not a good indicator of bean weight. Seed was collected in Chehalis from AVRDC breeding lines for use in future trials.
Carol A. Miles
New foods have been introduced to the North American populations from many world cultures in both planned and unplanned situations. Success of such crops will depend on the acceptance of the consumers. Growers and retailers must educate customers about these new crops in order to ensure that the customer comes back for more. The different strategies that can be used successfully in the process of education, marketing, and promotion, including developing recipe cards, brochures, newspaper and magazine press releases, and talks and presentations to local and regional groups, will be discussed.
Carol A. Miles and Jaqueline King
In this 2-year study of ‘Brown Snout’ specialty cider apple (Malus ×domestica) grafted onto Malling 27 (M.27) and East Malling/Long Ashton 9, we compared weight of total harvested fruit, labor hours for harvest, tree and fruit damage, and fruit and juice quality characteristics for machine and hand harvest. Machine harvest was with an over-the-row small fruit harvester. There were no significant differences due to rootstock; however, there were differences between years for most measurements. Weight of harvested fruit did not differ because of harvest method; however, harvest efficiency was 68% to 72% for machine pick and 85% to 89% for machine pick + clean-up weight (fruit left on trees and fruit knocked to the ground during harvest) as compared with hand harvest. On average for the 2 years, hand harvest required 23 labor-hours per acre at a total cost of $417, while machine harvest required 5 labor-hours per acre at a cost of $93. There were no differences due to harvest method on damage to spurs (four to eight spurs damaged per tree) or limbs (0.5–0.8 limbs damaged per tree). Although there were also no differences due to harvest method on fruit bruising (100% for both harvest methods in this study), 10% of fruit were sliced and 4% of fruit were cut in half inadvertently with machine harvest, and none were sliced or cut with hand harvest. Harvest method had no effect on fruit quality characteristics, specifically, soluble solids concentration (SSC), pH, specific gravity, titratable acidity (malic acid equivalents), or percent total tannin, when fruit was pressed immediately after harvest or stored for 2, 3, or 4 weeks before pressing. Juice quality characteristics were affected by storage, and SSC increased 11% in 2011 (3 weeks storage), and 12% and 18% in 2012 (2 and 4 weeks storage, respectively). Similarly, specific gravity increased both years after storage, 1% in 2011, and 1% and 2% in 2012 (a 1% increase in juice specific gravity corresponds to a potential 1.3% increase in alcohol by volume after fermentation for cider). Both years, juice pH tended to decline when fruit was stored (0.01 pH units in 2011, 0.06–0.12 pH units in 2012). Overall, cider apple harvest with an over-the-row small fruit machine harvester used four times less labor than hand harvest, yield reached 87% that of hand harvest (when clean-up yield was included), and juice quality characteristics were not negatively affected. These results suggest that machine harvest may be suitable for cider apples if equipment is available and affordable.
Sahar Dabirian and Carol A. Miles
The one-cotyledon splice grafting method is commonly used for watermelon (Citrullus lanatus) because it is relatively rapid and there is less rootstock regrowth than with other grafting methods. However, plants must rely on moisture in the air for survival during at least the first 4 days after grafting. In 2015 and 2016, greenhouse experiments were conducted to investigate if application of commercial stomata-coating and stomata-closing antitranspirant products, applied 1 day before grafting to both scion and rootstock seedlings, could increase the survival of watermelon transplants grafted using the one-cotyledon method. ‘TriX Palomar’ watermelon was grafted onto rootstock ‘Tetsukabuto’ (Cucurbita maxima × C. moschata) in Expt. 1, and onto rootstock ‘Emphasis’ (Lagenaria siceraria) in Expt. 2. The survival of grafted watermelon differed because of experiment (P = 0.0003), antitranspirant treatment (P < 0.0001), and experimental repeat (P < 0.0001). The survival of ‘TriX Palomar’ grafted onto ‘Tetsukabuto’ was greatest for plants treated with the stomata-coating + stomata-closing antitranspirants (92% to 100%), followed by the stomata-closing antitranspirant (79% to 97%), water (72%), and the stomata-coating antitranspirant (50% to 60%). For ‘TriX Palomar’ grafted onto ‘Emphasis’, plants treated with the stomata-closing antitranspirant had the greatest survival (87% to 97%), followed by stomata-coating + stomata-closing antitranspirants (84% to 94%), the stomata-coating antitranspirant (50% to 67%), and water (53% to 68%). In Expt. 3, stomatal conductance (g S) was similar for both ‘TriX Palomar’ and ‘Emphasis’ seedlings before treatment application, but differed because of the treatments 1 and 2 days after application. Stomatal conductance did not change for ‘TriX Palomar’ seedlings after application of the stomata-coating antitranspirant or water, or for ‘Emphasis’ seedlings after application of the stomata-coating antitranspirant. Stomatal conductance of ‘TriX Palomar’ seedlings decreased 57% to 62% after application of the stomata-closing antitranspirant and decreased 48% to 60% after application of the stomata-coating + stomata-closing antitranspirants. Stomatal conductance for ‘Emphasis’ seedlings increased 37% after water application, and decreased 58% to 68% after application of the stomata-closing antitranspirant, and decreased 42% to 45% after application of the stomata-coating + stomata-closing antitranspirants. The survival rate of grafted ‘TriX Palomar’ transplants was increased nearly 30% by application 1 day before grafting of the commercial stomata-closing antitranspirant or stomata-coating + stomata-closing antitranspirants in this study. Increase in grafting success is likely due to a reduction in transpiration that occurs when the stomata-closing antitranspirant is applied to the seedlings before grafting.
Sahar Dabirian and Carol A. Miles
Rootstock regrowth can prevent effective healing of grafted vegetable seedlings and outcompete the scion for light, space, and nutrients later in production. Rootstock regrowth is especially problematic for watermelon (Citrullus lanatus) because the crop is most commonly grafted using methods where meristematic tissue remains on the rootstock. The objective of this study was to test whether sucrose solutions [0% (water control), 1%, 2%, and 3%] applied as a drench to rootstock seedlings before grafting would increase the survival of watermelon grafted using the splice method where both rootstock cotyledons were removed to eliminate meristem tissue and rootstock regrowth. Starch accumulation in rootstock seedlings was the highest for plants that received 3% sucrose solution (71%), followed by plants that received 2% sucrose solution (52%), 1% sucrose solution (29%), and water (6%) (P < 0.0001). Survival (%) of splice-grafted watermelon seedlings 21 days after grafting was the greatest for plants that received 2% and 3% sucrose solution (89% and 82%, respectively), followed by plants that received 1% sucrose solution (78%), and was the lowest for plants that received water (58%) (P < 0.0001). There was a significant interaction due to repeat for both starch accumulation and grafted transplant survival; however, environmental conditions were similar for both repeats: the daily average temperature was 23 °C, the relative humidity (RH) was 64% to 67%, and the daily average light intensity was 224–243 µmol·m−2·s−1. Furthermore, while the vapor pressure deficit from 1:00 to 6:00 pm was 2.49 kPa for repeat 1 and 1.42 kPa for repeat 2, plant survival was greater in repeat 1 than repeat 2. These results indicate that drench applications of sucrose solution to rootstock seedlings before grafting can increase grafting success when both cotyledons are removed from the rootstock before grafting, but further research is needed to optimize the environmental conditions for the survival of grafted plants.
Sahar Dabirian, Debra Inglis and Carol A. Miles
Verticillium wilt, caused by the soilborne fungus Verticillium dahliae, is a significant disease affecting watermelon (Citrullus lanatus) production in Washington State. This field study at three locations in Washington in 2015 compared verticillium wilt susceptibility, fruit yield and quality of nongrafted watermelon, and grafted plants grown with black plastic and clear plastic mulch. Overall for grafting treatments, area under disease progress curve (AUDPC) values were higher for nongrafted ‘TriX Palomar’ (765) than for ‘TriX Palomar’ grafted onto ‘Super Shintosa’ (132), ‘Tetsukabuto’ (178), or ‘Just’ (187). Overall for mulch, the AUDPC value was higher for plants grown with black plastic mulch (385) than for plants grown with clear plastic mulch (237). Overall for location, the AUDPC value was lowest at Eltopia (84), intermediate at Othello (182), and highest at Mount Vernon (680). At season end, more Verticillium microsclerotia were present in stems of nongrafted ‘TriX Palomar’ than in grafted treatment stems at Eltopia and Mount Vernon, but not at Othello. Differences in microsclerotia presence occurred only in the top or scion portion of the stem, but not in the graft union, rootstock, or crown portions of the stem. There was no difference due to mulch in regard to Verticillium microsclerotia detected in stem assays. After harvest, V. dahliae soil density under black plastic mulch increased 6-fold at Eltopia, 4.7-fold at Othello, and 1.9-fold at Mount Vernon. In contrast, V. dahiae soil density under clear plastic mulch was nearly identical to the level at planting at each location (<1, 2.6, and 27 cfu/g at Eltopia, Othello, and Mount Vernon, respectively). There was a significant interaction between grafting and location for fruit yield such that there was no difference at Eltopia and Othello, but at Mount Vernon, yield of nongrafted ‘TriX Palomar’ was lower (7.4 kg/plant) than for grafted plants (average 13.0 kg/plant). The number and weight of marketable fruit per plant were higher at Othello (4.0 and 27.65 kg/plant, respectively) than at Eltopia (2.0 and 12.23 kg/plant, respectively) and Mount Vernon (2.2 and 11.63 kg/plant, respectively). Fruit firmness was greater overall for all three grafted treatments (average 2.67 N) than for nongrafted ‘TriX Palomar’ (2.20 N), but there was no difference in total soluble solids (TSS) or lycopene content of fruit due to grafting. Yield, fruit firmness, and TSS did not differ due to mulch type; however, lycopene content was greater for plants grown with black plastic mulch than with clear plastic mulch at Eltopia. There was no difference in TSS due to location, but fruit firmness was lower at Eltopia and Othello (2.20 and 2.44 N, respectively) than at Mount Vernon (3.00 N), whereas lycopene content was less at Mount Vernon (27.85 μg·g−1) than at Eltopia or Othello (38.58 and 36.54 μg·g−1). The results of this study indicate that although verticillium wilt symptoms were visible in watermelon plants when V. dahliae level was <3 cfu/g of soil, watermelon yield was not reduced. However, when V. dahliae soil density was >50 cfu/g of soil, yield was greater for grafted plants and for plants grown with clear plastic mulch.
Carol A. Miles and D. Gayle Alleman
Asian crops can provide growers with a means to diversify crop production and marketing options. However, before expanding into Asian crops, growers should determine consumer expectations regarding a new crop. Existing market criteria for each crop (i.e., maturity, color, size, shape) must be considered for all markets including traditional Asian use as well as for the general North American market. If growers decide to target general consumers in North America, then consumer awareness and acceptance must be addressed in a marketing and promotion program. Extension publications, popular magazines, and newspapers are useful tools in a marketing and promotion program. Crop production information must be available to enable growers to successfully produce Asian crops. Yet, most growers are unlikely to invest heavily in new production equipment and systems until a market has been established for the crop. It is a challenge for university scientists and extension agents to concurrently create supply and demand for new Asian crops. To accomplish this, multidisciplinary teams that include university and community experts should initiate a diversified program of Asian crop production, promotion, and marketing.
Sacha Buller, Debra Inglis and Carol Miles
Growth, fruit yield and quality, and potential tolerance to verticillium wilt (Verticillium dahliae) were compared among non-grafted, self-grafted, and grafted triploid watermelon (Citrullus lanatus Thunb., ‘Crisp’n Sweet’) and heirloom tomato (Solanum lycopersicum, ‘Cherokee Purple’). Rootstocks for watermelon were ‘Emphasis’ bottle gourd (Lagenaria sicerarea) and ‘Strong Tosa’ interspecific squash hybrid (Cucurbita maxima × Cucurbita moschata), and rootstocks for tomato were ‘Beaufort’ and ‘Maxifort’ interspecific tomato (Solanum lycopersicum × Solanum habrochaites). Field trials were carried out in 2010 and 2011 at Hermiston and Eltopia (eastern Oregon and Washington, respectively) and Mount Vernon (western Washington). Grafted watermelon had significantly larger stem diameter than non-grafted and self-grafted plants both years at Mount Vernon, whereas there were no differences at Hermiston or Eltopia. Grafted tomato in 2011 had significantly larger stem diameter than non-grafted and self-grafted plants at Eltopia and Mount Vernon, and ‘Beaufort’-grafted plants were significantly taller than other treatments at Mount Vernon. Grafting did not impact watermelon or tomato fruit yield or quality at any location either year. Foliar symptoms of verticillium were not observed on ‘Crisp’n Sweet’ watermelon at the eastern locations either year; however, at Mount Vernon, ‘Emphasis’ and ‘Strong Tosa’-grafted plants had significantly lower verticillium wilt severity than non-grafted and self-grafted plants both years. Microsclerotia were observed in all recovered watermelon stems sampled at Eltopia and Mount Vernon. V. dahliae was isolated from non-grafted and ‘Emphasis’-grafted ‘Crisp’n Sweet’ stems at Eltopia and non-grafted, self-grafted, and ‘Strong Tosa’-grafted stems at Mount Vernon. Foliar symptoms of verticillium wilt and microsclerotia in stems were not observed on ‘Cherokee Purple’ plants at either location both years despite site histories of the disease. Grafting with ‘Emphasis’ and ‘Strong Tosa’ rootstocks may be an effective strategy for managing verticillium wilt on watermelon in western Washington; however, grafting ‘Cherokee Purple’ onto ‘Beaufort’ and ‘Maxifort’ did not provide any advantages for tomato under the field conditions of this study.