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- Author or Editor: C.L. Guy x
The distribution of 14C-photosynthate was determined in 8-month-old potted ‘Valencia’ orange seedlings [Citrus sinensis (L.) Osbeck] during 25° and 10°C temperature regimes. Seedlings were pulse-labeled with 14CO2 for 3 hr after equilibration with ambient air. Radioactive assimilates were extracted at selected intervals, from leaves, stems, and roots and separated into several biochemical fractions. Low-temperature (10°) exposure resulted in a greater retention of 14C in the sugar fraction of leaves and a lower rate of 14C incorporation into organic and amino acid fractions. Data indicate a lower rate of metabolism of photosynthate and a reduced distribution of 14C in citrus seedlings at 10° than at 25°C.
In the paper, Distribution of l4C Photosynthetic Assimilates in ‘Valencia’ Orange Seedlings at 10° and 25°C by C. L. Guy, G. Yelenosky, and H. C. Sweet (J. Amer. Soc· Hort. Sci. 106(4):433–437. 1981) the title for Table 1 should read: l4C-labeled fractions extracted from ‘Valencia’ orange seedlings exposed to l4CO2 (50 μCi for 3 hours) at 25° and 10°C and maintained at those temperatures for 28 days in controlled temperature light rooms.
Randomly amplified polymorphic DNA (RAPD) markers were utilized to determine the genetic relationships of nine morphologically similar pot plant cultivars of Anthurium sp. by developing DNA fingerprints (DFP). Of 25 arbitrary primers screened, nine generated DFPs that were used in computing the genetic distance (d) and similarity coefficient (C) values. All cultivars tested exhibited a high degree of genetic similarity. `Lady Ann' and `Lady Beth' possessed the closest relationship with d and C values of 0.06 and 0.98, respectively. The next closest genetic relationship was between `Red Hot' and `Southern Blush' (d = 0.33, C = 0.89). These two cultivars exhibited a more distant relationship to the other seven cultivars as indicated by higher `d' values. However, this study showed that the nine Anthurium cultivars examined were genetically closely related. These cultivars share specific DNA bands with three possible parental species (A. andraeanum Linden ex Andre, A. antioquens L., and A. amnicola Dressler) included in this study, which may indicate similarities in their pedigree. This study shows that RAPDs can be a useful tool to distinguish Anthurium pot plant cultivars as well as identify their genetic relationships.