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Abstract
Callus cells were used as the cell source for isolating protoplasts of Salpiglossis sinuata L. Callus was initiated on the cut edges of leaf sections cultured on Uchimiya and Murashige (UM) medium under 24 μEm-2s-1 at 30°C. Friable callus from subcultures on UM was subjected to an enzymatic solution of 1% Driselase, 0.75% Pectinase, 2% Cellulase R-10, and 8% mannitol in CPW salts, and incubated at 50 rpm, for 4-5 hr to release protoplasts. Following washing, counting, and dilution, protoplasts were plated in liquid Murashige and Skoog medium (MS) modified by deleting the ammonium ions and adding (mg/liter): 250 L-glutamine, 0.1 L-serine, 2.0 thiamine, 0.5 indoleacetic acid (1AA), 1.0 2,4-D, 0.5 6-benzylamino purine (BA) and 9% mannitol. Within 2-5 days, cell wall synthesis and the first cell division occurred. Green-yellowish colonies, 2 to 3 mm in diameter, formed in 2.5 months and were transferred to MS + 1.0e mg/liter N6-isopentenyladenine (2iP), where shoot primordia were evident within 21 days. After full development and elongation of shoots, they were dipped in 1000 ppm indolebutyric acid (1BA) and placed in MS + 0.001 mg/liter 2,4-dichlorophenoxyacetic acid (2,4-D) to initiate roots. Rooting was carried out at 22±2°C under 80µm-2s-1 Cool White fluorescent tubes. Nine of 10 regenerated plants examined cytologically were 2n = 44, normal for the species, and 1 plant was 2n =88.
In 1993, then OSU President John Byrne declared that: “All Extension Service faculty, county agents as well as specialists, will be assigned academic colleges, and will have an academic appointment in the appropriate college.” The selection of the academic home would involve a mutual agreement between the individual and the department and would take into consideration the faculty member's academic training, experience, and work assignment. The implementation of this decision was completed by July 1995. In the College of Agricultural Sciences, this assignment of faculty to academic homes was accommodated by adding county agents to the faculty of existing departments. The Dept. of Horticulture faculty numbers nearly doubled, with an increase from 34 to 58. The department head is now very involved in the annual review and salary administration of extension field faculty. Campus-based faculty are now involved in all of the hiring, promotion, and tenure decisions for extension field faculty and vice versa. Field faculty participate in departmental decision making. The change in the number and diversity of faculty in the department is a unique effort to unify programmatic focus for extension, research, and teaching at OSU. As a work in progress, many issues are being addressed including full faculty participation in the decision-making process, communication, evaluation of scholarship, and building departmental community. Successes, pitfalls, and challenges ahead will be discussed and illustrated.
Abstract
Ericoid mycorrhizae were measured on roots of wild and cultivated highbush blueberries (Vaccinium corymbosum L.) from 7 locations in southeastern North Carolina over 4 sampling dates. An adequate clearing and staining procedure was developed specifically for observing endomycorrhizae in blueberry roots. Abundant mycorrhizae were found in wild blueberry plants, but few mycorrhizae were noted in cultivated plants. Phosphorus levels were lower in the wild than in the cultivated blueberry soils at most locations.