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- Author or Editor: Benjamin Jeyaretnam x
Changes in lipid and total protein content of somatic embryos of pecan (Carya illinoinensis) were estimated during maturation, cold treatment alone (3, 5, or 8 weeks) or cold followed by dessication (3, 5, or 7 days). Triglyceride was estimated colorimetrically and methyl esters of fatty acids were analyzed by GC-MS. Total protein was extracted from the same tissue with 2% SDS in Tris·HCL buffer. Triglyceride content of enlarged somatic embryos was significantly lower than zygotic embryos and further declined after 5 weeks cold treatment. An even greater decline was observed during the desiccation treatment. The most abundant fatty acids in small and enlarged somatic embryos are linolenic > palmitic > oleic > stearic acid. However, the molar ratio of linolenic to oleic reached 1:1 after 5 weeks of cold treatment. During enlargement, protein content increased to levels found in zygotic embryos, with desiccation resulting in further elevation.