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- Author or Editor: Benjamin Jeyaretnam x
Soluble and insoluble proteins from pecan (Carya illinoensis) zygotic and somatic embryos were resolved on one dimensional SDS-PAGE to study the changes in expression patterns of proteins. Soluble proteins were extracted using 0.1M NaCl and 50 mM Tris-HCl buffer while insoluble proteins were recovered by treating the pellet with SDS in Tris-HCl buffer. Several new insoluble proteins appeared during early maturation (26-47 days post anthesis, DPA) of zygotic embryos. The insoluble protein profile from early somatic embryos resembled that of cotyledon expansion stage zygotic embryos. About 12 groups of insoluble proteins are abundantly expressed after 27 DPA in zygotic embryos and constitute major storage and structural proteins. In contrast, storage proteins were absent in all somatic embryo stages. The intensity and number of bands of zygotic embryo soluble proteins declined with maturation and 11 prominent groups appeared around post-abscission stage.
Twenty accessions of Vernonia galamensis (Cass.) Less. from six subspecies were studied to develop a phylogram based on the seed protein profiles on one-dimensional (SDS-PAGE) gel electrophoresis. Soluble protein was extracted with 50 mM Tris-HCl buffer. Resolution factor values (Rf) and molecular weights of different protein profiles were estimated. The percentage similarities among different accessions were calculated and a consensus phylogram was derived using PAUP analysis. Almost all soluble proteins showed a moderate mobility (Rf. 0.2 to 0.6) and resolved as four clusters with varying number of classes. Variety ethiopica was observed to have greater mutational differences from other varieties of same subspecies galamensis and other subspecies. Variety petitiana showed greater genetic deviation to varieties galamensis, australis and nairobensis of the same subspecies galamensis than among the later three. Mutational distances between Subspecies gibbosa, mutomoensis and afromontana were similar.