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- Author or Editor: Becky B. Johnson x
Haramaki (1) reported that apical sections from vegetative shoots could be used to propagate gloxinia in vitro. The number of plants that could be produced from a single tuber was limited, however, since new vegetative shoots were individually placed on medium. More plants could be produced from a single tuber if leaf explants instead of shoots were used. In this paper I report an in vitro method to propagate gloxinia from leaf explants.
Unlike some gesneriads, such as gloxinia and Saintpaulia, where single plants are desired, a larger, more attractive mass of vegetation results when several Episcia plants per pot are used. This requires several cuttings per pot during propagation, increasing costs. An in vitro method for producing clusters of plants would thus be useful.
Leaf explants of broccoli [Brassica oleracea L. (Italica group)] produced either callus, roots, shoots, or both on Murishige and Skoog media (M&S) media with 4.0 to 6.0 mg/liter kinetin and from 8.0 to 9.0 mg/liter indoleacetic acid (IAA). Sections of leaf rib developed callus and then multiple shoots and roots on media with 8.0 to 10.00 mg/liter kinetin and 9.0 to 10.0 mg/liter IAA. Stem explants formed very little callus, but differentiated roots and shoots with 3.0 to 20 mg/liter kinetin and 9.0 to 10.0 mg/liter IAA. Successful transplanting to soil was achieved with all explants.