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  • Author or Editor: Ali Kuden x
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The similarity or differences of peroxidase isozymes in rootstocks and scions may influence their graft compatibility. This study was conducted to identify peroxidase isozymes that may be used as markers to predict compatibility between pear (Pyrus communis L.) and various quince (Cydonia oblonga Mill.) clones. `Bartlett' (BT) and `Beurre Hardy' (BH) pear cultivars are known to form incompatible and compatible grafts, respectively, with quince rootstocks. The two pear scion cultivars were budded on `quince A' (QA), `quince BA-29', and 15 selected quince clones from Turkey. Bark and cambial tissues were taken from nonbudded rootstocks and scions, and 4 cm above and below the graft union for peroxidase isozyme analysis performed by starch gel electrophoresis. Isoperoxidase analyses were also performed on samples from the graft unions collected 12 months after grafting. Many isozyme bands were observed commonly in the two scions; however, one anodal peroxidase A was detected in BH (compatible scion) but not in BT (incompatible scion) samples. This isoperoxidase was also detected in QA, Quince BA-29, and nine of the Turkish quince clones. Another isoperoxidase, band B, was detected in BH but not in BT or any of the rootstocks. However, the compatible (BH/QA) and moderately compatible (BT/BA-29) graft union tissues contained bands A and B whereas incompatible graft union tissues (BT/QA) lacked both. Graft union samples involving BT and five Turkish quince clones (705, 609-2, 702, 804, and 806) had both `A' and `B' isoperoxidases while one or both of these bands were absent in nonbudded graft partners. Field observations of 3.5 year-old grafts of BT and Turkish quince clones revealed that the vegetative growth (vigor) of BT scion was significantly greater, when grafted on these five clones, than that in graft combinations with other clones. We suggest that matching of isoperoxidase `A' in quince rootstocks and BH pear scion may be associated with a compatible graft combination. Additionally, presence of isoperoxidases `A' and `B' in the graft union tissues may be used as an indicator to predict a compatible graft between BT and quince rootstocks.

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The similarity or differences of peroxidase isozymes in rootstocks and scions may influence their graft compatibility. This study was conducted to identify peroxidase isozymes and/or other proteins that may be used as markers to predict compatibility between pear and various quince clones. `Bartlett' (BT) and `Beurre Hardy' (BH) pear cultivars were budded on 13 selected quince clones and quince A (QA) rootstocks; BT and BH cultivars are known to be incompatible and compatible, respectively, with quince root stocks. Bark and cambial tissues were taken from unbudded rootstocks, scions, and 4 cm above and below the graft union for isozyme analysis. Samples were collected 1, 2, 3, and 12 months after grafting. In addition, samples from the graft unions were also analyzed 12 months after grafting. Isozyme separation was performed by starch gel electrophoresis. Many isozyme bands were commonly observed in the two scions; however, one anodal peroxidase was detected in BH but not in BT samples. This isozyme was also detected in QA and in all but four quince clones. Protein profiles of bark tissues from QA and three pear scions (BT, `Bosc', and P. crassane) were determined using SDS-PAGE. In general, protein profiles of the three pear cultivars appeared remarkably similar; however, P. crassane (a compatible pear cultivar on QA) had a 63 kDa protein, which was absent in BT and faintly observed in `Bosc' (intermediate compatibility). Our results suggest that these isoperoxidase and polypeptide could be associated with pear/quince graft compatibility.

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