Drought stress reduces stem elongation and cell expansion. Since gibberellins (GAs) play an important role in controlling cell elongation, the objective of this study was to determine if the reduction in growth under drought stress is associated with altered GA metabolism or signaling. We exposed ‘Moneymaker’ tomato (Solanum lycopersicum) to drought stress to observe the effects on growth. Irrigation was automated using a data logger, which maintained volumetric water contents (VWC) of 0.35 and 0.15 m3·m−3 for well-watered and drought-stressed conditions, respectively. To further investigate the effect of GAs on elongation, paclobutrazol (PAC), a GA biosynthesis inhibitor, was applied to reduce endogenous GA production. Drought stress and PAC treatment reduced plant height. Internode length, cell size, and shoot dry weight displayed an interaction between the VWC and PAC treatments. The transcript levels of SlGA20ox1, -2, -3, and -4, SlGA3ox2, and SlGA2ox2, -4, and -5, corresponding to enzymes in GA metabolism, and LeEXP1, and -2, encoding expansin enzymes related to cell wall loosening necessary for cell expansion, were analyzed. Downregulation of transcript accumulation due to drought stress was observed for SlGA20ox4, SlGA2ox5, and LeEXP1, but not for any of the other genes. PAC increased expression of SlGA20ox-3, and SlGA3ox2, potentially through feedback regulation. These findings suggest that drought stress effects on elongation are at least partly mediated by altered GA metabolism.
Alexander G. Litvin, Marc W. van Iersel, and Anish Malladi
Alexander G. Litvin, Christopher J. Currey, and Lester A. Wilson
Broad-spectrum high-pressure sodium (HPS) lamps are the standard for greenhouse supplemental lighting. However, narrow-spectra light-emitting diodes (LEDs) offer potential benefits for enhancing growth, photosynthesis (P n), and secondary metabolites in culinary herbs. Our objective was to quantify the effect of supplemental light source and spectra on growth, gas exchange, aroma, and flavor of culinary herbs. Basil (Ocimum basilicum ‘Nufar’), dill (Anethum graveolens ‘Fernleaf’), and parsley (Petroselinum crispum ‘Giant of Italy’) were transplanted into hydroponic systems in a glass-glazed greenhouse. Plants were provided with a supplemental photosynthetic photon flux (PPF) density of 100 μmol·m−2·s–1 from an HPS lamp or LEDs with a low blue (B) to red (R) light ratio of 7:93 [low blue (LB)] or high B:R at 30:70 [high blue (HB)]. Compared with plants grown under HPS lamps, basil grown under LB and HB LED lighting was shorter, while only HB-grown parsley was shorter; height of dill was unaffected by light source. Basil and parsley shoot fresh weight was lower for HB-treated plants compared with HPS, though dill was unaffected by supplemental light source. Shoot dry mass of basil, dill, and parsley was unaffected by light source. Both LED treatments increased P n for basil and parsley compared with HPS-grown plants. Stomatal conductance (g S) was higher under LB and HB for basil compared with HPS in the morning and evening, but only HB-treated parsley was higher than HPS lighting in morning. Basil grown under LB, and parsley under both LEDs had lower chlorophyll fluorescence than those under HPS by the evening, but all three species had more chlorophyll b under LB light than HPS. Essential oil and phenolic accumulation were influenced by supplemental light treatment and responses varied among species. Lighting from LEDs resulted in a 2-fold increase in orientin and myristicin for basil and dill, respectively, while HB increased dillapiole concentration by 89% compared with HPS-grown dill. Notably, quercetin concentration was 2.8 times higher in dill grown under HB compared with HPS. Myrcene increased in all three species under either one (basil HB; dill LB) or both (parsley) LED lights compared with HPS. The increased content of aromatic and flavor compounds demonstrates the potential of supplemental lighting systems using specific wavelengths to add value; but the use of supplemental lighting requires an understanding of the additional stress on the photosynthetic mechanisms and the subsequent effect on biomass accumulation.
Nicholas J. Flax, Christopher J. Currey, Alexander G. Litvin, James A. Schrader, David Grewell, and William R. Graves
Various types of emerging bioplastic containers present a range of physical and chemical properties and can perform differently from one another in production environments. Container performance may be affected by substrate moisture content. We quantified the effects of bioplastic container type and substrate volumetric water content (VWC) on the aesthetic and mechanical strength properties of bioplastic containers and on plant growth. Seedlings of ‘Divine Cherry Red’ new guinea impatiens (Impatiens hawkeri W. Bull) and ‘Pinot Premium Deep Red’ zonal geranium (Pelargonium ×hortorum L.H. Bailey) were transplanted into five types of 11.4-cm–diameter containers, four types made from bioplastics and one type made from petroleum-based plastic and used as a control. Plants were watered to container capacity at transplant, allowed to dry down to VWC thresholds of 0.20 or 0.40 m3·m−3, and subsequently maintained at desired set points by using a precision irrigation system controlled by soil moisture sensors. Total volume of water applied per plant to new guinea impatiens was affected by VWC and not container type, whereas irrigation volume was affected by both for geranium. Growth index and shoot dry mass (SDM) of new guinea impatiens and geranium were affected by VWC. Container type affected growth index and SDM of geranium only. Water use efficiency (WUE) of both species was similar regardless of container type and VWC. Aesthetic quality varied based on VWC for only one container type, which was made from a blend that included soy-based bioplastic. Containers manufactured with polyhydroxyalkanoates (PHA) and dried distiller’s grains and solubles (DDGS) or polylactic acid (PLA), soy polymer with adipic anhydride (SP.A), and a proprietary bio-based filler (BR) derived from modified DDGS were stronger when maintained at a lower VWC, 0.20 m3·m−3. Our findings indicate that restricting irrigation to the minimum needed to achieve the desired crop growth is a viable strategy for sustaining aesthetic quality and strength of bioplastic containers manufactured with plant protein–based fillers such as SP.A and BR. Other bioplastic containers, such as those made of PLA–lignin biocomposite, show durability equal to that of petroleum-based plastic containers and maintain pristine appearance regardless of substrate VWC during production.