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  • Author or Editor: Akitsu Nagasawa x
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Morphogenically regenerable callus was induced from young leaf and meristem tissues of garlic (Allium sativum L. cv. Howaito-Roppen). Five auxins were compared for their ability to induce morphogenic callus. In order of decreasing effectiveness, 2,4-D (0.1–3.0 mg·liter−1), 2,4,5-T (0.3–10 mg·liter−1), dicamba (10–30 mg·liter−1), and picloram (10–30 mg·liter−1) were capable of morphogenic callus induction, while NAA did not induce morphogenic callus formation over a wide range of concentrations. The morphogenic callus was nodular and gave rise to plantlets following transfer to medium containing BA. Chemical names used: (2,4-dichlorophenoxy)acetic acid (2,4-D): (2,4,5-trichIorophenoxy)acetic acid (2,4,5-T); 3,6-dichloro-2-methoxybenzoic acid (dicamba); 4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid (picloram); 1-naphthaleneacetic acid (NAA); and N-(phenylmethyl)-1H-purin-6-amine (BA).

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