To evaluate the effects of plant density on gas exchanges under water stress resulting from high vapor-pressure deficit (VPD), we measured net photosynthetic rate (P n), transpiration rate, and leaf conductance (g l) of cucumber (Cucumis sativus L.) seedlings before and after raising the VPD at different plant densities. Measurements were conducted continuously using a chamber and weighing method. Five, nine, or 12 seedlings with leaf area index (LAI) of 0.39, 0.73, and 1.10, respectively, were placed in the assimilation chamber. The average VPD in the chamber was raised from 1.1 to 3.7 kPa 30 min after the starting measurement. The P n and g l decreased after raising the VPD above the plant community from 1.1 to 3.7 kPa. The VPD near the leaf surface (measured with 3-mm diameter humidity sensors) decreased with increasing LAI of the plant community, whereas average VPD in the whole chamber did not change with LAI. We noted significant negative correlations between the VPD near the leaf surface and P n and g l. These results indicate that higher plant density mitigates the inhibition of photosynthesis resulting from high VPD by maintaining a lower VPD near the leaf surface with the development of a thicker boundary layer above the canopy.
Toshio Shibuya, Akihito Sugimoto, Yoshiaki Kitaya, and Makoto Kiyota
Toshio Shibuya, Akihito Sugimoto, Yoshiaki Kitaya, Makoto Kiyota, Yuichiro Nagasaka, and Shinya Kawaguchi
We estimated leaf vapor conductance (g l) of cucumber grafted transplants under greenhouse growing conditions. Fifty-six transplants were placed on a bench in the greenhouse. The transpiration rate (Tr) of the canopy was estimated by weighing the 16 transplants in the center using an electronic balance. The total vapor diffusion resistance (R l+b) from inside the leaf to the atmosphere was estimated based on the vapor diffusion model, which incorporates the absolute humidity near the leaf surface and that inside the leaf as well as Tr. Next, g l was estimated from R l+b and the resistance of leaf boundary layer evaluated with a model leaf. The Tr in the afternoon tended to be larger than that in the morning at the same photosynthetic photon flux (PPF) level. By contrast, the g l in the afternoon tended to be smaller than that in the morning at the same PPF level. The decrease of g l in the afternoon seems to be induced by the excessive transpiration resulting from an increase of vapor pressure deficit at the leaf surface.