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The branched-chain amino acids (BCAA) leucine (L), isoleucine (IL), and valine (V) are synthesized in plants and are essential to growth in most organisms. These compounds can be absorbed by the plant when foliarly applied, but plant catabolism of BCAA is not completely understood. A recent study observed that BCAA applied in a 2:1:1 or 4:1:1 ratio (L:IL:V) increased creeping bentgrass (Agrostis stolonifera) shoot density compared with applications of equal urea nitrogen (N) at 3.03 lb/acre N. The present study investigated whether those increases could translate to a quicker establishment rate of creeping bentgrass grown from seed in standard greenhouse pots. The BCAA applications were compared with equal N applications using urea and a commercially available amino acid product. All N treatments were applied at 3.03 lb/acre N, per application and applied a total of four times on a 14-day interval starting 14 days after seeding. Measurements included final shoot density counts and root and shoot weights, as well as digital image analysis of percent green cover for each greenhouse pot every 7 days. No differences were observed after 70 days in shoot weight, or percent green cover between BCAA treatments and urea; however, BCAA 2:1:1 and 4:1:1 increased shoot density 21% and 30%, respectively, compared with urea, and were equal to the commercially available amino acid product. Applications of BCAA 4:1:1 also increased creeping bentgrass rooting weight by a factor of 7 compared with urea N.
Amino acids have been reported to improve turfgrass growth compared with mineral nutrition; however, amino acid catabolism in plants has not been well studied. A number of turfgrass fertilizers contain amino acids; however, some amino acids may be more effective additives in fertilizers than others. Three amino acids that could be effective nitrogen sources for plant growth are the branched-chain amino acids (BCAAs). The BCAA leucine (L), isoleucine (IL), and valine (V) could be effective additives because they are nonpolar and hydrophobic, which can promote plant uptake of these compounds. Although the effect of exogenously applied BCAA on plant growth is not well known, BCAAs have been reported to increase protein synthesis in humans, and that rate of increase is related to the intake ratio of L to IL and V. The objective of this study was to evaluate the use of L, IL, and V as a nitrogen sources on creeping bentgrass (Agrostis stolonifera) and to investigate the effect of BCAAs on plant growth when all three are applied as a combination. Using specially made rooting tubes, L, IL, and V were applied in a complete factorial and compared with equal urea nitrogen at four rates, as well as an untreated control. Where all three BCAAs were applied in combination, the application ratios of 2:1:1 and 4:1:1 (L:IL:V) were tested. At 63 days after seeding, there were no differences in root length, root weight, or shoot weight; however, BCAA 2:1:1 and 4:1:1 increased creeping bentgrass shoot density by 24% and 32%, respectively, compared with equal urea nitrogen. Where shoot density was increased, nitrogen application rate had no effect. On the basis of these results, BCAAs applied in a complete combination using ratios of 2:1:1 or 4:1:1 (3.03 lb/acre N) will provide a greater creeping bentgrass shoot density compared with equal urea nitrogen.
Soil degradation during construction often results in soil loss through erosion and reduced vegetation establishment. Composted organic materials are used to restore soil health of compromised urban soils when planting trees and shrubs; however, less is known about compost amendments for turfgrass establishment. The objective of this trial was to determine the effects of differing compost incorporation rates in two soil types on perennial ryegrass [PR (Lolium perenne)] establishment. The hypothesis was that as compost incorporation rates increase, turfgrass germination would increase until the compost rate becomes detrimental to turfgrass germination because of increased nitrate content and electrical conductivity (EC) levels. A salt-sensitive PR cultivar and a salt-tolerant PR cultivar were seeded into a loam topsoil and a clay subsoil at soil:compost volume ratios of 100:0, 80:20, 70:30, 60:40, 50:50, 40:60, and 0:100 using a mixed-source mature compost. Percent green cover (PGC) and leachate pH, EC, and nitrate content were measured during the 5-week establishment period. This trial showed that with a suitable topsoil, compost incorporation may be unnecessary to obtain acceptable PGC, but that compost additions (30% to 40%) to a clay subsoil achieve faster establishment while limiting the potential for reduced establishment because of increased nitrate content or EC associated with greater levels of compost incorporation. The EC or pH of soil:compost leachate was not found to predict turfgrass establishment. This trial suggests that soil type should be considered when making compost use rate recommendations; however, further research is needed to link compost physiochemical properties to compost use rates.
Aeration and sand topdressing are important cultural practices for organic matter management on golf course putting greens. Many golf courses lack the budget for applications of new sand topdressing material. A 2-year study was conducted to investigate the effect of recycling sand from hollow-tine aerification cores on a sand-based creeping bentgrass (Agrostis stolonifera) putting green soil properties and playability. Treatments included traditional [T (cores removed and sand topdressed)], verticut [V (cores broken up with verticutter)], and recycle [R (cores recycled using a core recycler)]. There were no differences in root zone organic matter, bulk density, soil porosity, infiltration rates, percent sand recovered during mowing, surface firmness, and ball roll distance between treatments during the study. Immediately after aerification treatments, T had the highest percent green cover (PGC) (38.3%) compared with V (26.9%) and R (26.8%), indicating that T offered the least sand present on the surface. Seven days after treatments, there was no difference in PGC (85.3% to 90.1%), indicating all treatments recovered similarly. Alternative aerification treatments V and R could be useful techniques to minimize or reduce the amount of sand used for backfilling core aeration holes without compromising the putting green soil properties and playability.
Data describing effects of plant growth regulator (PGR) applications on bermudagrass (Cynodon spp.) traffic tolerance are limited. A 2-year study was conducted evaluating effects of several PGRs on ‘Riviera’ bermudagrass (Cynodon dactylon L.) traffic tolerance. Treatments included 1) ethephon at 3.8 kg·ha−1; 2) trinexapac-ethyl (TE) at 0.096 kg·ha−1; 3) paclobutrazol at 0.28 kg·ha−1; 4) flurprimidol at 0.0014 kg·ha−1; 5) flurprimidol + TE at 0.0014 kg·ha−1 + 0.096 kg·ha−1, respectively; 6) ethephon + TE at 3.8 kg·ha−1 + 0.096 kg·ha−1, respectively; and 7) untreated control. All treatments were applied three times on a 21-d interval before trafficking. Plots were subjected to three simulated football games per week with the Cady Traffic Simulator. Traffic began 2 weeks after the last sequential application of each PGR. Turfgrass color, quality, and cover were quantified weekly using digital image analysis. Turfgrass cover measurements were used to assess traffic tolerance. Improvements in turfgrass color, quality, and cover were observed with applications of TE, ethephon + TE, and flurprimidol + TE. Turfgrass color, quality, and cover were enhanced for ethephon + TE and flurprimidol +TE compared with applications of ethephon and flurprimidol alone. Considering that no differences in turfgrass color, quality, or cover were detected among TE, ethephon + TE, and flurprimidol + TE at any time in the study, the responses observed suggest that TE may have a greater impact than other PGRs on ‘Riviera’ bermudagrass athletic field turf when applied before traffic stress. Chemical names used: rthephon (2-chloroethyl)phosphonic acid; glurprimidol {α-(1-methylethyl)-α-[4-(trifluoro-methoxy) phenyl] 5-pyrimidine-methanol}; paclobutrazol, (+/−)-(R*,R*)-β-[(4-chlorophenyl) methyl]-α-(1–1-dimethyl)-1H-1,2,4,-triazole-1-ethanol; trinexapac-ethyl [4-(cyclopropyl-[α]-hydroxymethylene)-3,5-dioxo-cyclohexane carboxylic acid ethyl ester].
Annual bluegrass (Poa annua L.) control with postemergence herbicides in cool-season turfgrass is often inconsistent. Amicarbazone and mesotrione have complementary modes of action but have not been evaluated in tank-mixtures for control of mature annual bluegrass in cool-season turfgrass. Field experiments were conducted during 2018 in New Jersey, and in Indiana, Iowa, and New Jersey during 2019 to evaluate springtime applications of amicarbazone and mesotrione for POST annual bluegrass control in cool-season turfgrass. On separate tall fescue (Festuca arundinacea Schreb.) and kentucky bluegrass (Poa pratensis L.) sites in 2018, three sequential applications of amicarbazone (53 g⋅ha−1) + mesotrione at 110 to 175 g⋅ha−1 provided >70% annual bluegrass control, whereas three sequential applications of amicarbazone alone at 53 and 70 as well as two sequential applications at 110 g⋅ha−1 provided <15% control at 14 weeks after initial treatment (WAIT). In 2019, results in New Jersey were similar to 2018 where amicarbazone alone provided less control than mesotrione + amicarbazone tank-mixtures. In Indiana, where the annual bluegrass infestation was severe and most mature, tank-mixtures were more effective than amicarbazone alone at 6 WAIT, but at 12 WAIT all treatments provided poor control. In Iowa, where the annual bluegrass infestation was <1 year old, all treatments provided similar control throughout the experiment and by >80% at the conclusion of the experiment. This research demonstrates that sequential applications of mesotrione + amicarbazone can provide more annual bluegrass control than either herbicide alone, but efficacy is inconsistent across locations, possibly due to annual bluegrass maturity and infestation severity.
Tall fescue (Schedonorus arundinaceus) offers an alternative to kentucky bluegrass (Poa pratensis) for use on athletic fields. Tall fescue has the ability to withstand athletic field traffic, but little is known about the best management practices such as optimal height of cut (HOC). A 2-year study was conducted on established ‘Snap Back’ tall fescue grown over a native soil root zone to determine optimal HOC under simulated athletic field traffic. Plots were maintained at various HOC treatments (1.5, 2, or 3 inches) for the duration of the growing season. Twenty-five simulated traffic events were applied each fall with a modified Baldree traffic simulator. The percentage of green cover (GC) loss per traffic event by HOC varied between years. In 2017, the 1.5-inch HOC improved traffic tolerance (–1.7% GC per event) compared with the other HOC treatments (–2.6% GC per event) in terms of percentage of GC. In 2018, the HOC did not have an impact on traffic tolerance. Differences in traffic tolerance between years could be a result of differences in precipitation (78 mm in 2017, 6 mm in 2018) during the period when traffic occurred, which suggest that the lower HOC performs better under wet conditions compared with the greater HOC. There were no differences among treatments for the safety variables measured (surface hardness, rotational resistance, and soil moisture).
Hybrid bermudagrass (Cynodon dactylon × C. transvaalensis) varieties such as Tifway and TifSport commonly are used on athletic fields. Several experimental hybrid bermudagrasses have been recently developed. However, data describing the performance of these bermudagrasses under simulated athletic field traffic are limited. A 2-year study was conducted evaluating the traffic tolerance of five experimental (2004-76, 2004-83, 2004-78, Tift 11, and 2004-77) and three commercially available (‘Tifway’, ‘TifSport’, and ‘TifGrand’) hybrid bermudagrasses. These bermudagrasses were subjected to two mowing (mowing at 0.87 inches or mowing at 0.87 inches + grooming to a 0.10-inch depth) and overseeding [no overseeding or overseeding with perennial ryegrass (Lolium perenne) at 12 lb/1000 ft2 of pure live seed] regimes. Simulated traffic tolerance using the Cady traffic simulator (CTS) was quantified using measurements of turfgrass cover with digital image analysis (DIA). Experimental bermudagrasses Tift 11 and 2004-76 and the commercially available variety TifGrand yielded turfgrass cover values greater than or equal to ‘Tifway’, a commonly used variety, on all rating dates each year. Experimental bermudagrass 2004-83 yielded the lowest turfgrass cover values on each date. Findings suggest that ‘TifGrand’, 2004-76, and Tift 11 may be suitable for use on athletic fields.