Mexican petunia (Ruellia simplex Wright) is a non-native plant that was introduced to Florida sometime in the 1940s and since then has naturalized in most of the state and in other southern states. Since 2007, we have developed at the University of Florida/Institute for Food and Agricultural Science in Gainesville the first Ruellia L. breeding program aiming to develop fruitless plants with different flower colors and growth habits that will not be invasive by seed dispersal. A combination of polyploidization and hybridization methods was used. In 2011, a total of 15 plants were selected and grown in southeastern, north–central, and northwestern Florida (Fort Pierce, Citra, and Quincy) using a randomized block design with three blocks and three plants per plot at each site. Plants were evaluated monthly for landscape performance, flowering, and fruiting. Two hybrids (R10-102 and R10-108) had outstanding potential as new fruitless cultivars for the plant industry having improved landscape performance and flowering.