Search Results

You are looking at 1 - 4 of 4 items for

  • Author or Editor: A. Fierro x
  • Refine by Access: All x
Clear All Modify Search
Full access

Rolston St. Hilaire and Carlos A. Fierro Berwart

The effects of 1H-indole-3-butyric acid (IBA), cutting position on stock plants, the date of propagation, the type of rooting substrate and temperature on rooting of mussaenda (Mussaenda erythrophylla Schumach. & Thonn. `Ashanti Blood' and `Rosea', and Mussaenda philippica A. Rich `Aurorae') stem cuttings were determined. Cuttings of `Ashanti Blood' produced the largest number of roots when treated with 15 mmol (3000 ppm) IBA and rooted in perlite at 29 °C (84 °F). Cutting position on stock plants did not affect rooting in any of the three cultivars. Propagation date and temperature of the rooting medium affected root numbers in `Aurorae'. With `Rosea', only the type of rooting substrate affected root number. Rooting percentage was 22%, 48%, and 39% in `Ashanti Blood', `Aurorae', and `Rosea' respectively. After 30 days of propagation average root length was 4, 12, and 4 mm (0.2, 0.5, and 0.2 inch) in `Ashanti Blood', `Aurorae', and `Rosea' respectively. Growers must determine precise rooting conditions for each cultivar to obtain consistent rooting of cuttings. This process may not be economically feasible on a commercial basis because rooting percentages are relatively low. We conclude that other methods of clonal propagation need to be evaluated before uniform rooted stem cuttings of mussaenda can be produced economically.

Free access

Rolston St. Hilaire and Carlos A. Fierro Berwart

Mussaendas (Mussaenda spp.) are ornamental shrubs, and some cultivars are difficult to root. This study was conducted to explore how adventitious roots initiate and develop in the cultivar Rosea, and to determine if anatomical events are associated with difficulty in rooting stem cuttings. Stem cuttings were treated with 5, 10, 15 mm 1H-indole-3-butyric acid (IBA), or distilled water, and sampled every 2 days over 26 days to observe adventitious root formation and development. For analysis by light microscopy, the basal 1 cm of cuttings was embedded in wax and stained with safranin-fast green. Adventitious roots initiated from phloem parenchyma cells and from basal callus in nontreated cuttings. Cuttings treated with 15 mm IBA had a mean of 18 root primordia per basal 1 cm of cutting after 10 days. Root primordia were not observed in non-treated cuttings at 10 days. Root primordia that developed in non-treated cuttings lacked clear vascular connections. These results suggest that non-treated cuttings are difficult to root because few primordia are produced.

Free access

A. Fierro, J. Norrie, A. Gosselin, and C.J. Beauchamp

Paper recycling generates large quantities of de-inking sludge, which is disposed of mainly by landfilling. More ecological disposal alternatives include land application and use as a container nursery medium. In this study, raw de-inking sludge was evaluated as a medium component supplemented with applications of four N fertilization regimes for the growth of three grass species (Festuca ovina duriuscula, Agropyron elongatum, Alopecurus pratensis), and four regimes of P fertilization for the growth of three Rhizobium-inoculated legumes (Medicago lupulina, Galega orientalis, Melillotus officinalis). Fertilizer was applied on the basis of sludge rate to maintain a uniform C: N ratio across sludge treatments. In one experiment, sand was mixed with 0, 10%, 20%, and 30% sludge by volume and 20% perlite, while in a second experiment, mineral soil was mixed with 0, 27%, 53%, and 80 % sludge and 20% perlite. Results indicate that shoot dry weight of all species increased with the amount of sludge in the mixture in tests with sand. In the soil mixture experiment, grasses showed the best response to treatments of 53% sludge mixture at the two highest N treatments. In general, shoot dry weight was more directly related to the total amount of N applied than to the C: N ratio of the substrate. The nutritional status (foliar N and P) also was investigated for one grass and one legume species.

Free access

Rolston St. Hilaire, Carlos A. Fierro Berwart, and Carlos A. Pérez-Muñoz

Mussaendas (Mussaenda spp.) are ornamental shrubs, and some cultivars are difficult to root. This study was conducted to explore how adventitious roots initiate and develop in the cultivar Rosea and to determine if anatomical events are associated with difficulty in rooting stem cuttings. Stem cuttings were treated with 5, 10, 15 mmol IBA, or distilled water, and sampled every 2 days over 26 days to observe adventitious root formation and development. Adventitious roots initiated from phloem parenchyma cells. Cuttings treated with 15 mmol IBA had a mean of 18 root primordia per basal 1 cm of cutting after 10 days. Primordia were absent in nontreated cuttings at 10 days. These results suggest that nontreated cuttings are difficult to root because few primordia are produced. Chemical name used: 1H-indole-3-butyric acid (IBA).