Allyl isothiocyanate (AITC) is a glucosinolate produced in cruciferous plant species. AITC is known to act as a pesticide on microorganisms, insects, and weeds. Synthetic AITC is registered as a biopesticide for agricultural soil treatment use in the United States and elsewhere in the world. Although a potent pesticide, reports on the weed and pathogen control efficacy of synthetic AITC applied as soil disinfectant are highly variable. Due to the low vapor pressure of AITC, questions remain as to whether pest and weed control efficacy can be improved by combining it with other chemicals. The objective of this study was to assess the control efficacy of AITC stand-alone applications vs. applications, in which AITC was combined with the standard-fumigants chloropicrin, 1,3-dichloropicrin, and methyl isothiocyanate. Two shank-applied on-farm field trials were conducted in cut flower [delphinium (Delphinium elatum), ranunculus (Ranunculus asiaticus)] fields, and two drip tape applied field trials in strawberry (Fragaria ×ananassa) fields in California. Weed pressure, weed seed viability, nematode survival, and pathogen survival of Pythium ultimum, fusarium wilt (Fusarium oxysporum), and verticillium wilt (Verticillium dahliae) were assessed. Cumulative yearly yield of marketable fruit was assessed in the strawberry field trials. The results of this study show that the use of AITC as a stand-alone treatment provided no consistent weed or pathogen control efficacy. However, our results also indicate that shank and drip applied multitactic fumigation approaches with AITC can efficiently control soil-borne diseases and weeds. These findings have potential implications, especially in those areas where certain fumigants are restricted due to regulations and/or availability.