Search Results
You are looking at 1 - 1 of 1 items for :
- Author or Editor: Sarah J. Anderson-Krengel x
- Journal of the American Society for Horticultural Science x
Abstract
Suspension-cultured pear (Pyrus communis ‘Bartlett’) cells cold-acclimated during exposure to 2°C. Cold acclimation was accompanied by changes in soluble extracellular polysaccharides and in the deposition of callose in the cell wall. Release of a relatively small neutral polysaccharide into culture medium was increased at 2°. However, low temperature decreased the extracellular accumulation of a larger molecular weight (M r) pectic polysaccharide. The reduced amount of pectic polysaccharide may have been the result of both a low-temperature-enhanced degradation of existing polysaccharide and an inhibition of new synthesis or secretion. The effect of low temperature on callose deposition was observed using an aniline blue fluorescent staining procedure. Pear cells held at 2° showed far more intense staining than those at 22°, indicating increased deposition of callose or other β-1,3-glucans at the cell surface during cold acclimation.