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  • Author or Editor: S.A. Miller x
  • Journal of the American Society for Horticultural Science x
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Abstract

Four annual applications of Ca(NO3)2 were made to ‘Golden Delicious’ and ‘Delicious’ apple trees beginning in the second leaf at rates of 0.5 to 8 times the recommended rate (45 g/tree per year of age) in each of three soil management systems: cultivated, herbicide, and mowed sod. Soil samples were collected in Mar. and July 1983 (fifth leaf) to a 120-cm depth at the tree drip line. July samples were analyzed for available Ca, Mg, K, Mn, percentage of base saturation, NO3-N, and soil pH (0.01 m CaCl2); March samples only for NO3-N. The sod system had the highest soil pH, Ca level, and percentage of base saturation; the herbicide had the lowest, and the cultivated treatment was intermediate. The rate of Ca(NO3)2 had no measurable effect on these values except in the surface pH levels (030 cm), where sod was unaffected but the cultivated and herbicide systems had a significant reduction in pH with increasing levels of Ca(NO3)2. Soil Mn availability and leaf Mn increased with increasing Ca(NO3)2 levels in association with the decreasing pH levels. Available soil Mg and leaf Mg decreased with increasing Ca(NO3)2 due to Ca displacement of soil Mg on the cation exchange complex. Leaf Ca was unaffected by Ca(NO3)2 rate or the soil management system. During the growing season (Mar.-July 1983), the herbicide system accumulated significantly more of the applied Ca(NO3)2 than the cultivated or sod systems. No yield response and minimal leaf NO3 response suggested differences in NO3 accumulation were due to variation in leaching due to the soil management systems.

Open Access

Abstract

Application of a complete nutrient solution (CNS) on apple seedling leaves reduced stomatal conductance (gs). Tween 20 and CaCl2 were components of the CNS which induced gs reduction. Tween 20 alone, however, did not cause stomatal closure, but CaCl2 (24.8 mm) had a consistent, negative effect on gs when applied alone. Application of CaCl2 in combination with one of the other macrocomponents of the CNS (MgSO4, urea, or K2SO4 + KH2PO4) produced less consistent gs reductions indicating that the CaCl2 effect on gs can be modified by the presence of these compounds. Urea, MgSO4, or K2SO4 + KH2PO4 had little effect on gs when applied separately. Application of MgCl2 or KCl, which were not the CNS components, decreased and had no effect on gs, respectively. In addition to gs reduction, CaCl2 sprays reduced net photosynthesis (Pn). The equivalence of intercellular CO2 concentration in sprayed and unsprayed seedlings implied that the Pn drop following CaCl2 sprays resulted from decreased capacity of mesophyll for CO2 fixation and not from reduction in the stomatal aperture. Two possible explanations for stomata closure are discussed: a direct effect of CaCl2 on stomata and an indirect effect of CaCl2 spray through changes in mesophyll CO2 fixation capacity. Reductions in gs and Pn following treatments with different salts were not associated with visible leaf injury.

Open Access

Crosses were made between tomato (Lycopersicon esculentum Mill.) inbreds susceptible to races T2 and T3 of bacterial spot (Xanthomonas vesicatoria and Xanthomonas campestris pv. vesicatoria, respectively) and accession PI 114490 with resistance to races T1, T2, and T3. Resistance to race T2 was analyzed using the parents, F1, and F2 generations from one of the crosses. The F1 was intermediate between the parents for disease severity suggesting additive gene action. The segregation of F2 progeny fit a two-locus model (χ2 = 0.96, P = 0.9-0.5) where four resistance alleles are required for a high resistance level, two or three resistance alleles provide intermediate resistance, and zero or one resistance allele results in susceptibility. The narrow sense heritability of resistance to T2 strains was estimated to be 0.37 ± 0.1 based on F2 to F3 parent-offspring regression. A second cross was developed into an inbred backcross (IBC) population to facilitate multilocation replicated testing with multiple races. Segregation for T2 resistance in the inbred backcross population also suggested control was by two loci, lending support to the two-locus model hypothesized based on the F2 segregation. To determine if the same loci conferred resistance to the other races, selections for race T2 resistance were made in the F2 and F3 generations and for race T3 resistance in the F2 through F4 generations. Six T3 selections (F5), 13 T2 selections (F4's that diverged from seven F2 selections), and control lines were then evaluated for disease severity to races T1, T2, and T3 over two seasons. Linear correlations were used to estimate the efficiency of selecting for resistance to multiple races based on a disease nursery inoculated with a single race. Race T1 and race T2 disease severities were correlated (r ≥ 0.80, P< 0.001) within and between years while neither was correlated to race T3 either year. These results suggest that selecting for race T2 resistance in progeny derived from crosses to PI 114490 would be an effective strategy to obtain resistance to both race T1 and T2 in the populations tested. In contrast, selection for race T3 or T2 will be less likely to result in lines with resistance to the other race. PI 114490 had less resistance to T3 than to T2 or T1. Independent segregation of T2 and T3 resistance from the IBC population derived from PI 114490 suggests that T3 resistance is not controlled by the same genes as T2 resistance, supporting the linear correlation data.

Free access

A tissue culture protocol was developed that increased the germination percentage and decreased the lag time to germination for strawberry (Fragaria x ananassa Duch.) achenes. This technique involved cutting surface-sterilized achenes across the embryo axis then placing the shoot apex/radicle-containing sections on semisolid Murashige and Skoog medium lacking hormones. Cut achenes began germinating 5 days after culture and achieved maximum germination (97% to 100%) in less than 2 weeks, compared to whole achenes, which began to germinate 7 to 10 days after sowing and required more than 7 weeks for maximum germination (<50%). Enhanced germination of cut achenes was a general phenomenon since achenes from 231 hybrid crosses responded similarly. Following placement on culture medium, cut achenes could be stored up to 8 weeks at 4C then removed to 27C, where germination and seedling development occurred at percentages and rates comparable to freshly cut achenes. Achenes did not require stratification before cutting to exhibit increased germination. Nearly 100% of the achenes from freshly harvested red-ripe, pink and white strawberries germinated after cutting and culture, although cut achenes from white and pink berries germinated more slowly than those from red-ripe berries. Achenes from green berries, whether whole or cut, did not germinate. This method of “embryo rescue” could be used to generate more seedlings from poorly germinating hybrid crosses, would considerably decrease the time from sowing to seedling production compared to traditional means, and would produce seedlings of uniform age for subsequent field evaluation.

Free access

Studies were conducted on peach [Prunus persica (L.) Batsch] during 1988 to 1990 to test the performance of a tree-width rope-curtain bloom thinner and a rotating rope-curtain thinner. Six trips over the tree canopy were required with the tree-width rope curtain, and only one trip was required with the rotating curtain to thin to a spacing of about one flower per 9 cm of fruiting shoot length. Based on the number of flowers per square centimeter of branch cross-sectional area (CSA) immediately following thinning and the number of fruit per square centimeter of CSA following June drop, rope-curtain thinning was equal to hand-thinning at full bloom (FB). Rope-curtain thinning reduced hand-thinning time by 40% and increased harvest fruit weight by 10% to 20%. Research on various modifications in tree training/pruning indicated that performance of the mechanical thinner was negatively correlated with shoot density. Thinning was maximum on open-center-trained trees on which detailed pruning had been conducted to eliminate overlapping shoots.

Free access