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  • Author or Editor: Ronald G. Goldy x
  • Journal of the American Society for Horticultural Science x
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Abstract

Three pentaploids produced from hybridizing hexaploid Vaccinium ashei Reade and diploid V. darrowi Camp were analyzed for fruit set, number of seeds/fruit, seedlings/pollination, pollen grains/sporad, 2n gamete production, pollen germination, leaf area and berry weight, color, scar, and firmness. The pentaploids were intermediate between the parental species in fruit size and leaf area. There was high variation in fertility and in fruit quality among the pentaploid hybrids.

Open Access

Abstract

Methods of producing 8x plants from 4x V. corymbosum clones through in vitro colchicine treatments were investigated. Colchicine concentrations and exposure times ranged from 0.001% to 0.2% and 1 to 14 days, respectively. Pretreatments designed to predispose the plants to the effect of colchicine also were investigated. Eleven 8x plants and one 4x-8x chimera were selected from pretreated plants exposed to 0.025% or 0.05% colchicine. The superior treatment was 0.025% with a 24- or 48-hr exposure time in liquid medium. No differences were noted between warm/dark and cold/dark pretreatments. No differences were observed among 24-, 48-, 96-, and 192-hr growth intervals between cold pretreatment and colchicine treatment. However, a significant genotype effect in response to colchicine was found, both with respect to culture survival and polyploid induction.

Open Access

Abstract

Pollen morphology of 10 Vaccinium species, 10 interspecific hybrids, and 3 colchicine-induced polyploids were studied using scanning electron microscopy. Size, exine patterning, furrow and pore size and shape, and general appearance were investigated. Although differing morphological features were generally lacking, some interspecific differences and variation within hybrid populations were noted. Furrow and pore sizes, and the associating opericuli were the most valuable characters. Some species had large furrows with large rounded pores and others had small furrows with insignificant pores. Exine patterning was minimal and, when present, generally occurred in the inter-colporate regions. F1 populations of V. corymbosum × V. elliottii had the greatest exine variation and the degree of exine patterning revealed a possible dosage effect. Exine patterns suggest that the ancestors of V. myrsinites may be V. darrowi and V. elliottii rather than V. darrowi and V. tenellum as previously proposed by Camp.

Open Access