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  • Author or Editor: Ronald G. Goldy x
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Four muscadine grape (Vitis rotundifolia Michx.) cultivars (Carlos, Noble, Regale, and Tarheel) were evaluated for their ability to be cultured in vitro. Axillary buds were placed on Murashige and Skoog medium as modified by Chee. Different levels of benzylaminopurine [(BA) 0.5 to 10.0 μm], kinetin [(KIN) 0.5 to 5.0 μm], and thidiazuron [(TDZ) 0.5 to 11.3 μm], and different explant positions were evaluated for their effect on in vitro explant establishment and shoot production. Thidiazuron (2.3 to 4.5 μm) alone or in combination with BA (1.0 to 5.0 μm) or KIN (1.0 or 5.0 μm) was effective for establishing axillary buds. Similar levels were also effective for promoting shoot proliferation. Explants originating from the 10 basal nodes of a shoot with at least 25 nodes gave better shoot proliferation than explants originating from the 10 distal nodes. Chemical names used: 6-benzylaminopurine, 6-furfurylaminopu. rine (kinetin):N -phenyl-N'-l,2,3 -thiadiazol-5-y lurea (thidiazuron).

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Abstract

Flower buds of blackberries, blueberries, grapes, raspberries, and strawberries were observed in order to determine the relationship of morphological development and meiosis. Blueberries showed the least development whereas grapes and brambles had considerable development prior to meiosis. Meiotic figures were found with relative ease in blueberries, strawberries, and grapes but with difficulty in brambles.

Open Access

Abstract

Four isozyme systems, glucosephosphate isomerase (PGI, EC 5.3.1.9), phosphoglucomutase (PGM, EC 2.7.5.1), isocitrate dehydrogenase (IDH, EC 1.1.1.42), and malate dehydrogenase (MDH, EC 1.1.1.37), were identified as useful in detecting intergeneric Vitis vinifera × Muscadinia rotundifolia hybrids. Polymorphism between and within the two genera was observed at the PGI-2, PGM-2, and IDH-1 loci. However, the two appeared fixed for different alleles at the MDH-3 locus. The combination of any two of the enzyme systems allowed for rapid identification of F1 hybrids at the young, pre-fruiting seedling stage.

Open Access