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  • Author or Editor: Rita Biasi x
  • Journal of the American Society for Horticultural Science x
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One- and three-node nectarine explants were compared with intact potted units of similar dimension. The explants and intact plants performed similarly as judged by rate of leaf photosynthesis, leaf and fruit respiration, and changes in fresh and dry weights. Water loss and transpiration were less in explants than intact plants after 24 h. Explants with fruit of nectarine, olive, and prune were used to evaluate uptake and distribution of 14C-labeled paclobutrazol (PBZ), daminozide, and sucrose in plant parts. These comparisons reveal that the explant system is useful for primary testing of hypotheses, screening of chemicals, and evaluating species response for later testing of selected parameters in the field. Three-node explants containing fruit are reliable for experiments lasting up to 4 days. Chemical names used: succinic acid 2,2 dimethylhydrazide [daminozide (SADH)]; β-[(4-chlorophenyl)methyl]-α-(1,1-dimethylethyl)-1H-1,2,4-triazole-1-ethanol [paclobutrazol (PBZ)].

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Abstract

Containerized peach [Prunus persica (L.) Batsch.] seedlings were grown in the greenhouse at three water levels [25%, and 100% field capacity (FC)] with experiments duplicated in Bologna, Italy and Davis, Calif. One group of the seedlings was treated with 0.1 g active ingredient (a.i.) paclobutrazol (PBZ) applied as a soil drench, whereas the second group received water only. Addition of PBZ suppressed shoot growth and leaf area more than reduced water content alone. PBZ reduced root fresh and dry weights and total water consumption. At 0600 and 1200 hr, PBZ increased stomatal conductance at 100% FC; later that same day stomatal conductance decreased. At 50% and 25% FC, PBZ decreased stomatal conductance compared to controls at all times measured. Chemical name used: (2RS, 3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pentan-3-ol (paclobutrazol).

Open Access