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  • Author or Editor: R. M. Skirvin x
  • HortTechnology x
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A method has been developed for micropropagation of the difficult-to-root winegrape cultivar `Norton' (Vitis aestivalis). Plants were established in vitro from axillary bud cuttings of field-grown plants. Four levels of 6-benzylaminopurine (BA) and three levels of naphthaleneacetic acid (NAA) were tested in a factorial arrangement for their effectiveness in promoting multiplication of shoots from single-node explants. Three levels of NAA and two concentrations of Murashige and Skoog (MS) basal medium were tested for their effectiveness in promoting rooting of shoot tips. The greatest number of shoots per axillary bud in combination with the greatest shoot length were produced with 4 μmol·L-1 [0.90 mg·L-1 (ppm)] BA. NAA had no effect on shoot multiplication. NAA was not required for in vitro rooting. All rooted plants survived the transition to soil.

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Extensive root development was observed on Stage 2 `Red Emerald' philodendron shoots grown on standard multiplication medium consisting of Murashige and Skoog salts and vitamins with 6-benzylaminopurine (BA, 0.2 mg·liter-1. Root development was suppressed significantly when the level of NH4NO3 was doubled from standard levels (lx, 1650 mg·liter-1) to 3300 mg·liter-1 (2×). A higher level of NH4NO3 (3×, 4950 mg·liter-1) was detrimental to shoot growth and proliferation. This information may be useful for commercial propagators who wish to suppress root development at the shoot multiplication stage.

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Acclimatization and growth of in vitro-derived apple shoots of two apple scion apple cultivars were compared under fogged conditions in a greenhouse and in a commercial growth cabinet (Phototron). Plant survival rates of microcuttings of `Royal Gala' and `Jonagold' were significantly better when maintained in the Phototron units than when grown in a greenhouse under fog. The number and length of roots on microcuttings was significantly higher in the Phototron than under fog. In the present study, we demonstrated that the Phototron environment was better than a fogged greenhouse for establishing apple shoots ex vitro. However, the Phototron units are so small that they hold no more than 100 to 120 plants at a time. Therefore, the units will be of most value to growers or individuals in laboratories who do not have a constant need for acclimatization facilities. Growers who acclimatize many plants should continue to use fogging or misting facilities.

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