Eleven muscadine grape (Vitis rotundifolia Michx.) cultivars were evaluated in Nov. and Dec. 1994 and Jan. and Feb. 1995 for primary bud hardiness using differential thermal analysis. Vines sampled were located at the University of Arkansas Fruit Substation, Clarkeville. Data analysis indicated a significant date × cultivar interaction for mean low temperature exotherm (LTE). Mean LTE decreased for all cultivars during the sampling period. Hardier cultivars for November and December were `Sterling' and `Summit'; for January, `Nesbitt', `Summit', `Carlos', and `Sterling'; and for February, `Sugargate', `Carlos', and `Summit'. The minimum LTE for any cultivar was – 23.8C for `Sugargate' during February.