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- Author or Editor: Nian Wang x
- HortScience x
Leaf discs of Salpiglossis sinuata L. were infected with disarmed Agrobacterium tumefaciens strain LBA 4404 carrying β-glucuronidase (GUS) gene and kanamycin resistance. Plantlets were regenerated from the leaf discs cultured on Murashige and Skoog (MS) medium supplemented with 10 μM kinetin and 1 μM naphthaleneacetic acid (NAA) plus 300 mg/liter kanamycin. The plantlets were then rooted on the MS medium containing the same concentration of kanamycin plus 2 μM NAA without kinetin. The histochemical assay showed that the regenerated plants were GUS positive. A Southern blot test is underway to determine the stability of the transferred genes in the regenerated plants.
Rhododendron delavayi Franch. is an important ornamental plant and often plays a role in natural hybridization with other sympatric species in Rhododendron subgenus Hymenanthes. Fifteen microsatellite loci were developed and characterized in this species. The average allele number of these microsatellites was four per locus, ranging from three to six. The ranges of expected (HE ) and observed (HO ) heterozygosities were 0.0365 to 0.7091 and 0.0263 to 0.9512, respectively. Cross-species amplification in R. agastum and R. decorum showed that a subset of these markers holds promise for congeneric species study. These sets of markers are potentially useful to investigate the genetic structure and gene flow of R. delavayi and other congeneric species.
Cleistogamy in Salpiglossis sinuatu L. involves a sequence of events, including arrested corolla development, precocious pollen germination inside anther, pollen tube penetration of the pistil, and eventual self fertilization, that takes place. within a tightly closed flower bud. A single dominant gene (C) controls cleistogamy in this plant. During early blooming period, cleistogamous (CC, Cc) plants produce both chasmogamous (open) and cleistogamous (closed) flowers. Enzymes in various tissues of both cleistogamous and chasmogamous buds were detected by isozyme banding patterns in starch gel electrophoresis. The onset of cleistogamy may be signalled in the calyx and corolla tissues in the early stage of flower development. The levels of specific enzymes (PGM, PGI, G-6PD, PGD, MPI) involved in gluconeogenesis, pentose phosphate shunt and glycolysis in both calyx and corolla tissues of the cleistogamous buds were greatly reduced. These enzymes were present in the pistil and anthers of cleistogamous buds and in all floral parts of the chasmogamous buds.