Search Results

You are looking at 1 - 2 of 2 items for :

  • Author or Editor: Ni Lee x
  • Journal of the American Society for Horticultural Science x
Clear All Modify Search
Authors: and

Plantlets were recovered from axillary bud cultures of muscadine grape (Vitis rotundifolia, `Summit'). Nodal segments 0.5 to 1.0 cm long were cultured in Murashige and Skoog (MS) basal medium supplemented with 5, 10, 20, or 40 μm BA. Best total shoot production was obtained with 10 μm BA; with higher BA levels, shoots were unexpanded and exhibited high mortalities. MS medium supplemented with IBA enhanced rooting by increasing rooting percentage and number per plantlet. Shoots previously proliferated on medium with 5 μm BA rooted significantly better than those multiplied on 10 μM BA. Shoot vigor during rooting was greater in shoots proliferated on 5 vs. 10 μm BA. Root development was not significantly affected by liquid vs. agar-solidifted medium or shoot length. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA), 1H-indole-3-butyric acid (IBA).

Free access


The anatomy of in vitro- and in vivo-developed leaves of sweetgum, Liquidambar styraciflua L., grown under three quantum fluxes (PPF), was evaluated using light and scanning electron microscopy. Leaf characteristics of both in vitro- and in vivo-developed plants were modified by light: high irradiance was associated with more compact mesophyll and larger cells than low irradiance. However, when compared to plants grown in vivo under corresponding irradiance levels, all plants grown in vitro had smaller, thinner leaves and smaller mesophyll cells lacking extensive vacuolar components. Leaves developed in vitro had larger, raised stomata regardless of light level and, except at the highest irradiance, exhibited significantly greater stomatal densities than in vivo-developed leaves.

Open Access