An investigation of free amino acids, phenols, and indoles from various plant parts of double (DD and Dd) and single (dd) flowered petunia plants was made to determine if a relationship exists between these substances and flower genotype. There was no difference between genotypes in total free amino acid concentration from shoot tips or anthers. Flower buds with the DD genotype and leaves with the Dd genotype were significantly different in total amino acid concentration from similar tissue of the other genotypes. TLC analysis of amino acid extracts from shoot tips, buds, or leaves failed to resolve any qualitative differences between the 3 genotypes. Anthers directly squashed on TLC plates and developed in several solvent systems did not resolve any differences between genotypes. TLC analyses of phenols and indoles indicated they were very similar for the 3 genotypes.
The external surfaces and internal structures of particles of milled pine bark (Pinus taeda L. and P. elliottii Engelm.) were examined with scanning electron microscopy. Numerous external openings, cracked cell walls and internal cellular connections, that might allow water penetration were observed. Periderm surfaces were without pores, and contained rough surfaces and apparently waxy substances that might resist water penetration or absorption.