Search Results
You are looking at 1 - 3 of 3 items for :
- Author or Editor: N. F. Weeden x
- HortScience x
Abstract
Two triploid apple (Malus domestica Borkh.) cultivars, ‘Spigold’ (‘Red Spy’ x ‘Golden Delicious’) and ‘Jonagold’ (‘Golden Delicious’ x ‘Jonathan’), and their parental cultivars were analyzed for a number of isozyme systems by starch gel electrophoresis. The banding pattern and intensity of one isozyme system, 6-phosphogluconate dehydrogenase (6PGD), provided strong evidence that the parent contributing the 2n gamete in both 3x cultivars was the female.
A DNA extraction protocol was developed for tissues from woody species. DNA was extracted successfully from 11 species and five different types of tissues and was suitable for RAPD and restriction analysis. Spermine precipitation was used to further purify DNA. The protocol can be used for large-scale analysis and mini-preparations.
Abstract
Isozyme phenotypes were used to identify two (P2 and P3) of the six monosomic alien addition lines that have been isolated from a Cucurbita moschata × C. palmata hybrid. Phenotype P2 displayed the C. palmata fumarase isozyme, whereas P3 exhibited two glucose phosphate isomerases and an aspartate aminotransferase derived from C. palmata. P2 also possessed the hard rind trait characteristic of C. palmata. Both the biochemical and the morphological phenotypes were inherited in a non-Mendelian fashion, and no recombination was observed within either the P2 or P3 set of characters. It was concluded that the loci coding fumarase and hard rind were situated on the alien chromosome in P2 trisomics and that the other three loci were on a 2nd C. palmata chromosome possessed by the P3 line. The loci responsible for other C. palmata isozymes either were not expressed or were not located on any of the five C. palmata chromosomes represented in the alien addition lines.