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- Author or Editor: Marilyn C. Erickson x
- HortScience x
Temperature stress resulting in membrane-associated disorders has been linked to lipid peroxidation through free radicals, but the susceptibility of membrane lipids in microsomal fractions to chilling stress has not been clearly delineated. Microsomal membranes isolated from bell peppers were subjected to five oxidative conditions (iron-ascorbate, xanthine oxidase, cumene hydroperoxide, and lipoxygenase (LOX) with and without PLA2) at three temperatures (6, 21, and 37C) and measured at 30 min. intervals during a 2 hour incubation to determine the effect of temperature on peroxidation as measured by TBA-RS. During the first 30 min., the rate of peroxidation was greater at 6C than at 21C or 37C in four of the five systems tested. Preincubation with PLA2 followed by addition of LOX produced the highest amount of peroxidative products when compared with all other systems demonstrating the importance of free fatty acids in lipoxygenase-catalyzed peroxidation. The results demonstrate an increased susceptibility of microsomal membranes isolated from a chilling-susceptible fruit to peroxidative challenge at chilling temperatures than at higher temperatures.