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  • Author or Editor: Margrethe Serek x
  • HortScience x
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Somatic embryos of cyclamen [Cyclamen persicum Mill.] were produced using a liquid culture system. Two encapsulation techniques, conventional alginate beads and alginate hollow beads, were tested for globular cyclamen somatic embryos with the aim of developing synthetic seeds. Final germination from alginate beads was as high as observed for non encapsulated control embryos (97%), but germination was delayed. In contrast, germination from hollow beads was lower (71%) and occurred later. In hollow beads somatic embryos developed within the capsule, and outgrowth seemed to be more difficult than from alginate. Storage at 4 °C for four weeks resulted in a reduction of viability for controls as well as for encapsulated embryos. Incorporation of medium into the capsules improved the speed of germination for both capsule types. However, somatic embryos were not able to germinate on a medium-free support, even if encapsulated in beads containing medium.

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Postharvest life of Rosa hybrids L. `Elegance Parade' was increased by 0.2 mm silver thiosulfate and decreased by ethephon at 500 μl·liter-1. In contrast, spraying the plants with various polyamine (PA) concentrations (spermine, spermidine, or putrescine) or the PA-synthesis inhibitor methylglyoxal-guanylhydrazone did not affect postharvest life. Investigation of PA-uptake patterns showed that the compounds were entering the petal tissue.

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Miniature rose (Rosa hybrida L. cv. Victory Parade) plants were treated with AOA, BA, or STS before simulated shipment and display in an interior environment. Although AOA-treated plants lasted slightly longer than nontreated plants, their postproduction quality, evaluated as floral longevity, bud drying, and bud abscission, was not as satisfactory as that of STS- or BA-treated plants. In this ethylene-sensitive cultivar, BA treatment was almost as satisfactory as STS treatment. Flowers that opened in the greenhouse before shipping lasted longer than those that opened in the interior environment room. STS and BA treatments increased the longevity of both flower types. However, these treatments did not eliminate the difference between flowers that opened before or after transport simulation. Chemical names used: aminooxyacetic acid (AOA); benzyladenine (BA); silver thiosulfate (STS).

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Spraying flowering plants of Schlumbergera truncata (Haw.) `White Christmas' with aminoethoxyvinylglycine (AVG), an inhibitor of ethylene biosynthesis, did not prevent the rapid loss of flower buds caused by exposure to 1 μl of ethylene/liter. Treatment with the silver thiosulfate anionic complex (STS) strongly inhibited such effects. The rate of bud drop in ethylene-free air (interior environment room) was somewhat reduced by AVG treatment, although total display life of treated plants was not significantly different from that of the controls. STS treatment reduced the rate of bud drop, and increased display life by 20 %.

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The vase life of cut sunflowers given a simulated transport period (3 days dry storage at 8C) was significantly enhanced by a l-hour pulse with 0.01% Triton X-100 administered before storage. The Triton pulse increased solution uptake during the l-hour pulse, decreased fresh weight loss during dry storage, and significantly improved water uptake thereafter, resulting in greater leaf turgidity and longer vase life. Leaf stomata] conductance measurements indicated that Triton X-100 maintained stomatal opening at a higher level during the pulse and after storage, but had no effect during dry storage. Chemical name used: octylphenoxypolyethoxyethanol (Triton X-100).

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