Water uptake in different regions of the sweet cherry fruit (Prunus avium L. cv. Sam) was investigated following selective application of silicone sealant to the pedicel end, pedicel cavity, pedicel/fruit juncture, or stylar scar of detached fruit. The time course of water uptake was monitored gravimetrically during a 3-hour incubation period in deionized water (20 °C). Sealing the pedicel end and/or pedicel/fruit juncture significantly reduced rates and total amount (3 hours) of water uptake, but sealing the stylar scar had no effect. The amount of water penetrating via the pedicel/fruit juncture increased between 50 and 85 days after full bloom. During the same period the maximum force required to detach pedicels from fruit (fruit removal force) fell from 5.2 ± 0.5 to 2.1 ± 0.2 N. The amount of water penetrating via the pedicel/fruit juncture and the fruit removal force were negatively related. Nuclear magnetic resonance (NMR) imaging of mature fruit incubated in D2O indicated that D2O accumulated in the pedicel cavity region and the pedicel. Our data suggest that the pedicel end and pedicel/fruit juncture, but not the stylar scar, are regions of preferential water uptake in detached fruit. Chemical name used: deuterium oxide (D2O).