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- Author or Editor: Leonard L. Morris x
- Journal of the American Society for Horticultural Science x
Abstract
Controlled atmosphere (CA) storage prolonged the shelf life of mushrooms (Agaricus bisporus, [Lange] Sing.) if the O2 concentration was 9% or the CO2 concentration was 25 or 50%. Concentrations of 2 to 10% O2 stimulated pileus expansion and stipe elongation with maximal stimulation of growth occurring at 5% O2. Levels of CO2 above 5% markedly inhibited growth, even after air was substituted for the CO2 treatment. Five percent CO2 stimulated stipe elongation and suppressed pileus expansion. Protein degradation, as indicated by protease activity and the level of a-amino N in the tissue, increased during postharvest maturation of mushrooms. As in starving bacteria, it is suggested that the main physiological function of proteolysis in the postharvest maturation of mushrooms is as a source of C and N.
Abstract
Postharvest discoloration of cultivated mushrooms (Agaricus.bisporus [Lange] Sing., ‘tan strain’) was significantly retarded by treatment with succinic acid-2,2-dimethylhydrazide (SADH). The optimum SADH concn was 100 ppm. The effect, however, lasted no longer than 3 days after which time all SADH treatments discolored at rates equal to or greater than controls. The decrease in discoloration was correlated with a decrease in o-diphenol oxidase (o-DPO) activity. Protease activity was higher in SADH treated mushrooms suggesting that reduction in browning was due to degradation of o-DPO rather than direct inhibition of o-DPO by SADH. In vitro SADH competes with proline for quinones produced by enzymatic or non-enzymatic oxidation of diphenols. It is proposed that in vivo SADH exerts a dual effect in reducing mushroom discoloration: first SADH induces degradation of o-DPO through an increase in proteolytic activity, and second it binds to quinones thereby removing intermediates which lead to pigment formation.
Abstract
Storage of mushrooms (Agaricus bisporus, [Lange] Sing.) in 0% O2 reduced discoloration and o-diphenol oxidase (o-DPO) activity for up to 7 days. Levels of O2 above 0% had little or no effect in reducing discoloration and o–DPO activity compared to air controls. Concentrations of CO2 above 5% appeared to increase surface discoloration while markedly inhibiting o-DPO activity. After transfer to air, the effectiveness of the increased CO2 treatments in reducing o-DPO activity in mushrooms depended on storage time in CO2.
In vitro CO2 markedly inhibited o-DPO activity, with 50% inhibition at 25% CO2. Complete inhibition was never attained. The inhibition by CO2 was found to be competitive with respect to catechol and could not be overcome by increasing the O2 concentration above 20%. The action of CO2 in vivo in reducing o-DPO activity could be through a direct competitive inhibition or through the inhibitory effect of CO2 on mushroom maturation.
Abstract
Mushrooms [Agaricus bisporus, (Lange) Sing.] stored at 10° and 20°C showed a sigmoid pattern of growth while at 0°C growth was retarded. The postharvest growth exhibited at 10° and 20°C could be related to a decrease in free a-amino N while at 0°C there was a significant increase in the level of free a-amino N during storage. Protease activity in the tissue increased at all 3 temperatures. It is suggested that postharvest maturation of mushrooms is supported by utilization of low molecular weight nitrogenous compounds formed through increased protein degradation. Mushrooms stored at 20°C toughened and matured faster than those stored at 10° or 0°C. Increases in discoloration during storage appeared to be correlated with decreases in total phenols and with increases in o-diphenol oxidase (o-DPO) activity. The relationship of these biochemical changes to postharvest maturation of mushrooms is discussed.
Abstract
Parthenocarpic cucumber fruit (Cucumis sativus L. cv. Deliva) of marketable maturity (10 to 14 days after anthesis) were held at 12.5° or 20°C in reduced O2 levels for 5 or 18 days before transfer to air. Carbon dioxide production at reduced O2 levels was generally less than in air; however, at O2 levels < 0.5%, anaerobic respiration resulted in increased rates of CO2 production. Upon transfer to air after 18 days, all samples from reduced O2 showed increased CO2 production rates that equalled or exceeded that of the air controls. Except at 0.0% and 0.25% O2 levels, ethylene production was increased in reduced O2. After transfer to air, ethylene production increased and the increase was inversely related to the previous O2 level. Ethanol and acetaldehyde production were measureable for fruit held in 1% O2 after 18 days at 12.5° and showed dramatic increases at lower O2 levels. Low-O2 injury (pitting) developed on most fruit held at 0.0% O2 and on many fruit held at 0.25% O2. Only minima! commercial benefits are likely to be realized from storage of 1 to 3 weeks in 0.5% to 2.0% O2 at 12.5°.
Abstract
The incidence of brown stain on crisphead type lettuce (Lactuca sativa L. cv. Calmar) was highly dependent on postharvest temperature. Following exposure to 2% O2 + 5% CO2 for 5 days at constant temperatures from 0° to 20°C, brown stain decreased as simulated transit temperature increased and was negligible at 10°C and above. Simulated market temperatures following exposure to 2% O2 + 5% CO2 at 0°C markedly influenced brown stain expression with near maximum expression present after a 3-day period in air at 10°C. Low O2 (2%) was more effective in reducing respiration (CO2 production) of lettuce at temperatures above 5°C than at 5°C, or lower.
Abstract
The severity of brown stain on crisphead lettuce (Lactuca sativa L., cvs. Calmar and Great Lakes 118) held at 0° or 2.5°C for 10 days increased with increasing CO2 (1 to 5%) and decreasing O2 (21 to 1%) levels. Very slight or no brown stain developed on lettuce subjected to 0, 1, or 2% CO2 in combination with 10 or 21% O2 for 10 days or on that held in 2.5 or 5% CO2 + 2% O2 for 4 days at 2.5°C. In 2.5 or 5% CO2 + 2% O2, brown stain intensified with duration of exposure (2 to 30 days) at 0° or 2.5°C.
Abstract
Sensory evaluations and chemical analyses were used to investigate the effects of various postharvest handling procedures on composition and flavor quality of ‘Cal Ace’ tomatoes (Lycopersicon esculentum Mill.) harvested at the mature-green and light-pink stages. Ethylene treatment to speed ripening of green tomatoes at 20°C resulted in a higher reduced ascorbic acid content at the table-ripe stage and did not influence flavor when compared with fruits ripened without added ethylene. Using a low-O2 atmosphere to retard ripening had less of an effect on flavor than stage of ripeness at harvest. No differences were found between fruits where ripening was delayed by using 4% O2-atmosphere at 20° or by using low temperature (12.5°). Exposing fruits to 5° for 7 days before ripening at 20° affected flavor; i.e., chilled fruits were more acid. Above the chilling range (0-12.5°); duration of holding after harvest was more important than storage temperature. Lower holding periods resulted in loss of characteristic “tomato-like” flavor and development of “off-flavors.” Mature-green fruits, ripened at 20° under restricted air flow, had increased “off-flavors” when compared to those ripened under accelerated air exchange. Light-pink fruits subjected to impact bruising before ripening had more “off-flavor” and less “tomatolike” flavor than those without physical damage. Quantitative differences in a few volatile components were found with certain treatments, but no qualitative differences were detected and there was no significant difference in total volatile content among any of the treatments tested.
Abstract
Four amino acids (glutamic, γ-aminobutyric, glutamine, and aspartic) make up about 80% of the total free amino acids in fruits of tomato (Lycopersicon esculentum Mill., cv. Cal Ace). Fruits harvested at the table-ripe stage contained more alanine and less glutamic acid than those picked green or at the breaker (incipient red color) stage and ripened at 20°C to table-ripe. The higher glutamic acid concentrations in fruit picked at the breaker or earlier stages were paralled to higher scores for “off-flavor,” as described by a taste panel, relative to fruits picked table-ripe. However, when monopotassium glutamate (60, 120, or 180 mg/l00g) was added to diced table-ripe fruits, the panelists were not able to detect flavor differences due to increased glutamic acid concn. Differences in amino acid composition associated with fruit ripeness when picked do not appear to be directly related to flavor differences.
Abstract
Composition and sensory characteristics were investigated to determine the effect of ripeness at picking on fresh market flavor of ‘Cal Ace’ (1974, 1975, 1976) and ‘Cherry’, ‘Calmart’, and ‘Early Pak 7’ (1976) tomato (Lycopersicon esculentum Mill.). Tomatoes picked at earlier stages of ripeness and ripened at 20°C were evaluated by panelists as being less sweet, more sour, less “tomato-like” and having more “off-flavor” than those picked at the table-ripe stage. Objective tests showed these fruits had less sugars and reduced ascorbic acid, and varied significantly in volatile composition. The magnitude of these differences varied greatly among the cultivars. In ‘Cal Ace’ the “off-flavor” characteristic was largely correlated with a volatile compound (peak 43) but in other cultivars seven other volatile compounds also appeared to play a role.