Knowledge of the genes underlying a given trait is highly useful for developing molecular markers for breeding and is the foundation for future genomic crop improvements. The cultivated strawberry, F. ×ananassa, is a valuable horticultural crop. Genome sequencing revealed that of the four diploid strawberry subgenomes contributing to the F. ×ananassa octoploid genome, the woodland strawberry, F. vesca, subgenome is dominant. Thus, F. vesca is an important system for determining gene function and should be used as a source of genetic diversity for F. ×ananassa breeding. Ethyl methanesulfonate mutagenesis of H4 F7-3, an inbred line of F. vesca, resulted in one M2 line that did not produce any strawberries over a 3-year period in the greenhouse. This line was named fruitless 1. The fruitless 1 phenotype results from a single gene recessive mutation. Microscopic characterization revealed that fruitless 1 failed to produce fruit because anthers fail to develop properly before meiosis, resulting in no pollen production. This report of fruitless 1 facilitates further studies of the line.