Seven open-pollinated pecan [Carya illinoinensis (Wangenh.) K. Koch] stocks were grown in a nursery in blocks. Bud growth of ungrafted seedlings was influenced by rootstock, with growth being more advanced on `Curtis', `Elliott', `Apache', and `Sioux' seedlings than on `Moore', `Riverside', and `Burkett'. Bud growth of grafted trees was influenced by scion, with growth of `Candy' being most advanced, while `Cape Fear' trees were more advanced than `Stuart'. Growth of `Candy' grafted trees was affected by rootstock, with growth being more advanced on `Elliott' and `Curtis' seedling rootstock as compared to `Apache', Sioux', `Riverside', and `Burkett' seedling rootstock. Tree damage caused by a May freeze was directly related to bud growth and was influenced by scion and rootstock.
Precocity of pecan [Carya illinoinensis (Wangenh.) C. Koch] seedlings (year of first fruit production) was studied in relation to original seed measurements (nut weight, buoyancy, volume, and density) and in relation to growth index (GI) measurements of seedling trees for 4 years. A total of 2,071 pecan seedlings, representing nine controlled-cross families, were studied. Original seed measurements were not related to precocity of resultant seedling trees; but seed weight, buoyancy, and volume were significantly correlated with seedling growth rates. Nut density was negatively related to growth of seedlings. These relationships show the importance of original seed measurements and seed parentage in determining seedling growth, and have direct relevance in pecan nursery operations to increase general rootstock seedling vigor. Seedling growth rate was significantly correlated to precocity levels, with measurements taken in the later years of the study showing the highest correlations with precocity. This strong growth-precocity relationship may have negative genetic implications since a common breeding objective is to produce more precocious cultivars that maintain smaller tree size in mature orchards.
Putative resistance to the blackmargined aphid (Monellia caryella Fitch) in `Pawnee' pecan [Carya illinoinensis (Wangenh.) K. Koch] was first noted in greenhouse tests by rating cultivars for relative amounts of honeydew on adaxial leaf surfaces. This resistance was confirmed in two field tests monitored from mid-June to mid-October. `Pawnee' supported significantly lower aphid populations during every rating period when relatively large numbers of these insects were present. `Navaho' also showed resistance, with `Desirable' having intermediate resistance and `Stuart' being very susceptible. Insect populations were also monitored on the four quadrants of each tree, with quadrant effect being significant in only one test. This test had the highest populations on the western quadrant and lowest populations on the eastern quadrant. In determining individual clone resistance, it is recommended that the general orchard aphid infestation level be determined so that only two or three well-timed clonal ratings are needed. We also recommend that all sides of the tree be monitored.
Leaf anatomical traits of Mexican and U.S. pecan [Carya illinoinensis (Wangenh.) K. Koch] seedstocks grown in a single location were studied to determine patterns of ecogeographic variation within the natural range. Stomatal density was uniform among open-pollinated seedlings of a common maternal parent with twofold differences in stomatal density separating some seedstocks. There was an inverse relationship between stomatal density and epidermal cell density. Stomatal density and stomatal index of Mexican seedstocks were related to longitude and annual precipitation of origin. Stomatal density increased along the longitudinal gradient toward the east coast of Mexico; seedstocks originating from areas on the east coast of Mexico had greater stomatal density than seedstocks originating from the drier areas on the west coast. Stomatal density and stomatal index did not follow a pattern along latitude or longitude in the U.S. seedstocks. Although isotopic carbon (13C) discrimination did not vary greatly in Mexican seedstocks, the reduction in stomatal density in pecan trees from areas with reduced annual precipitation suggest the presence of an anatomical feature to reduce water losses.
Adaxial and abaxial leaflet surfaces of juvenile and adult pecan [Carya illinoensis (Wang.) K. Koch] leaves were characterized in relation to leaf age. Three types of trichomes were found. Nonglandular hairs (acicular and fasciculate) were the most common trichome type on juvenile-phase leaves and reached their greatest density on the abaxial surface of immature leaves. Peltate scales were present in two forms: vesicular scales and concave scales. The two were distinguished on the basis of size and shape. Both types were more common on the abaxial than the adaxial surface. The vesicular scales were more common on immature than mature leaves, whereas the occurrence of concave scales were unaffected by leaf age. Capitate glands were observed on the veins of immature leaves in both juvenile and adult phase. Laminai stomata of two sizes and unusual veinal stomata were observed, the latter being confined to the abaxial surface of immature leaves.
The Munsell Color System was used to define pecan [Carya illinoinensis (Wangenh.) K. Koch] kernel colors and color changes for 21 clones, 11 locations, and 4 storage methods for nuts collected over a 4-year period. Hue readings ranged from 10.0 (10 red) to 22.5 (2.5 yellow). Value readings ranged from 2.5 to 8.0, and chroma readings ranged from 1.0 to 8.0. A total of 91 color chips (individual combinations of hue, value, and chroma) were needed to describe kernel color variability. In 1987 and 1988, one color [15.0/5/4 (hue/value/chroma)] accounted for 3,979 of the 32,078 readings taken, and the 15 most common colors accounted for 80.7% of all the readings. The Munsell system of color determination was well suited for pecan color determinations. A simplified color rating system with only six color classes was developed for general use by the pecan industry. This system is also routinely used in our breeding and genetics program to define this very important quality trait in pecan.
A microsatellite-enriched library was developed from `Halbert', a native pecan [Carya illinoinensis (Wangenh.) K. Koch] selection from Coleman County, Texas. A genomic library enriched for simple sequence repeats (SSR) containing 6144 clones was archived in 384 well plates for screening. In total, 439 clones were identified after Southern hybridization using di- and tri-nucleotide repeats as probes. In total, 125 positive clones were sequenced and primers were designed for 24 repeats. The SSR markers chosen for analysis include di-(CT and GA) and tri-nucleotide repeats (CTT, GAA and GAT). Of the 24 primer pairs tested, 19 successfully amplified microsatellites from `Halbert'. DNA was isolated from 48 pecan and hickory accessions selected to strategically represent the genetic diversity of the National Clonal Germplasm Repository (NCGR) Carya collections. The 19 SSR primers that produced good amplification products in `Halbert' were used to evaluate the collection, with 11 revealing polymorphism. The number of fragments amplified with different primer combinations ranged from 4 to 32 in the 48 genotypes tested. Evaluation of the data confirms the utility of the microsatellites in delimiting known relationships.
The Munsell color system was used to study kernel color differences between four pecan [Carya illinoinensis (Wangenh.) K. Koch] cultivars (`Cheyenne', `Choctaw', `Western', and `Wichita') grown at four locations (Tulare, Calif., and Brownwood, Crystal City, and El Paso, Texas) during two seasons (1987 and 1988) and stored under different temperatures (20 to 24 °C and -5 °C). Kernel color changed over time from yellow to red hues and from lighter to darker values, but changed very little in chroma. Initial ratings of each color attribute by cultivar were positively correlated with patterns of change in that attribute over time. Kernels collected in 1987 were more yellow and had greater color saturation than kernels collected in 1988. `Cheyenne' kernels were the most yellow of the cultivars tested and `Wichita' kernels were the most red. `Cheyenne' kernels were lighter than those of any other cultivar. Kernels frozen 6 or 12 months were more red in hue than unfrozen kernels, but could not be distinguished on the basis of value (lightness). Kernels frozen 12 months had reduced chroma compared to those frozen 6 months or unfrozen. Shelled kernels of `Wichita' changed hue more in storage than kernels of other cultivars. Shelled kernels held at 20 to 24 °C became darker and developed red coloration quicker than unshelled pecans. Variation in hue and value accounted for the majority of color difference between cultivars. Changes in hue accounted for the majority of color change over time. Differences among cultivars in value (lightness) were consistent over time.