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  • Author or Editor: Kee Yoeup Paek x
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We report the results of serial studies aimed at clarifying several factors affecting organogenesis in rhizome culture of temperate Cymbidium species and their hybrids. The growth patterns and regeneration ability of rhizomes derived from asymbiotic seed or shoot tip culture vary according to media composition, kinds and concentrations of plant growth regulators, culture conditions, and species and varieties. N6-benzyladenine was the best cytokinin for inducing shoot formation, for switching rhizome tissues into protocorm-like bodies, and for directly forming multiple shoots from branched rhizomes. Activated charcoal appeared to be necessary for producing healthy plantlets and for stimulating shoot growth at levels of 0.1% to 0.3% but concomitantly decreased rhizome growth. Sucrose at 5% was the most effective concentration for shoot induction from rhizomes. The above results support the conclusion that organogenic pathways between tropical, subtropical, and temperate Cymbidium species may be controlled by the genetic backgrounds of the species or cultivars.

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Adventitious buds regenerated from homogenized leaf tissue of Saintpaulia ionantha Wendl. `Crimson Frost' were micropropagated to determine types and frequencies of the variants obtained. Plants grown for one year in a greenhouse showed 67% variation and 33% normality. A higher rate of variation was observed in leaf color rather than in leaf shape. The variations in leaf color and leaf shape were 67% and 19%, respectively. In regard to flower type, greater numbers of semidouble and double types were obtained as compared to single types. Both flower types showed a much higher rate of normal (mixed) color (81%) as compared to pink, red or white (19%). An sodium dodecylsulfate polyacrylamide gel electrophoresis profile of protein extracted from leaves of the stock plants and the variants indicated no difference between them and did not reflect the variation in phenotype.

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