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  • Author or Editor: Jack W. Buxton x
  • Journal of the American Society for Horticultural Science x
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Abstract

A method is described for determining glucose metabolism by the C-6/C-1 ratio. After an initial lag period to allow for equilibration of labelled glucose, an increase of accumulated 14CO2 in rose petals was linear with time. By taking the limit of the ratio of the C-1 and C-6 lines, one can arrive at the C-6/C-1 ratio as a ratio of the slopes of the lines. The slope of each of the lines can be calculated with 2 determinations within a relatively short time period. This method reduces metabolic recycling of the glucose and other problems associated with the traditional method of determining the C-6/C-1 ratio.

Open Access

Abstract

The pentose phosphate shunt (PPS) was shown to be active in roses (Rosa hybrida L.). The C6/C1 ratio indicated that possibly as much as 50% of the glucose oxidized in the rose flower is through the PPS. However, the activity of the PPS in relation to the Embden-Meyerhof-Parnas pathyway-tricarboxylic acid cycle (EMP-TCA) pathway did not change significantly throughout the cut life of the rose. There was a drop in the respiratory rate of petals throughout the cut flower life. The inner petals exhibited a consistantly higher respiratory rate than did outer petals regardless of whether the flower was kept in water or preservative.

Open Access