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  • Author or Editor: J. L. Anderson x
  • Journal of the American Society for Horticultural Science x
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Abstract

Leaves of cold-acclimated lemon [Citrus limon (L.) Burm. f.], grapefruit (C. paradisi Macf.), orange [C. sinensis (L.) Osbeck], and mandarin (C. unshiu Marc.) trees ranged in cold hardiness from −4 to −11°C. No significant differences in water content (g H2O/g dry weight) or melting point depression were observed. Plots of liquid water content during freezing (g H2O/g dry weight) vs. temperature were similar for the 4 citrus species. The tissues apparently deviated from ideal freezing behavior because less ice was formed. The reduced ice formation could not be accounted for by osmotic effects. Negative pressure potential developed during freezing is hypothesized to play a role in tissue water potential in frozen systems. It was concluded that hardier Citrus leaves survive freezing of a larger fraction of their tissue water.

Open Access

Abstract

Mature ‘Red Delicious’ apple trees (Malus pumila Mill) were sprinkled intermittently with an overhead irrigation system after completion of winter rest. A 2-minute sprinkling cycle operated automatically whenever the ambient air temperature of the orchard exceeded 7°C until the control trees reached full bloom. Evaporative cooling of the treated trees reduced bud temperatures to within 2°C of the wet bulb temperature. Treated trees reached full bloom 17 days after the untreated controls.

Open Access

The hypersensitive response in resistant plants exposed to incompatible pathogens involves structural changes in the plant cell wall and plasma membrane. Cell wall changes may include pectin deesterification resulting in release of methanol. The time course of methanol production was characterized from `Early Calwonder 20R' pepper (Capsicum annuum L.) leaves infiltrated with the incompatible pathogen, Xanthomonas campestris pv. vesicatoria (Doidge) Dye race 1 (XCV). In the first time course experiment, leaves were infiltrated with either 108 colony-forming units/mL of XCV or water control. Leaf panels (1 × 5 cm) were excised after dissipation of water soaking, then incubated in vials at 24 °C. Headspace gas was analyzed at 6-hour intervals up to 24 hours. The rate of methanol production from resistant pepper leaves infiltrated with XCV was greatest during the first 12 hours after excision. In another experiment, leaf panels were harvested at 6-hour intervals up to 24 hours after inoculation and incubated for 12 hours at 24 °C to determine the relationship between the interval from inoculation to leaf excision and methanol production. The highest rate of methanol production was obtained when the interval between bacterial infiltration and leaf excision was 18 hours. The relationship between methanol release and changes in the degree of methylesterification (DOM) of cell wall pectin was determined in near isogenic lines of `Early Calwonder' pepper plants resistant (20R) and susceptible (10R) to XCV race 1. Cell walls were prepared from resistant and susceptible pepper leaves infiltrated with XCV or water. XCV-treated resistant leaves had 18% DOM and 9.7 nmol·g-1·h-1 of headspace methanol, and the susceptible leaves had 48% DOM with 0.2 nmol·g-1·h-1 methanol. Susceptible and resistant control leaves infiltrated with water had 55% and 54% DOM, respectively, with no detectable methanol production. Increased methanol production in resistant pepper leaves inoculated with XCV coincided with an increase in cell wall pH. Intercellular washing fluid of resistant pepper leaves had a significantly higher pH (6.9) compared to susceptible leaves (pH 5.1) and control leaves infiltrated with water (pH 5.1). Both 10R and 20R pepper leaves infiltrated with buffer at increasing pH's of 5.1, 6.9 or 8.7 had increased methanol production. Since deesterified pectin is more susceptible to degradation, demethylation may facilitate formation of pectic oligomers with defensive signalling activity.

Free access