Bare-root transplants received from high latitude nurseries for Florida production have limited root systems, very long petioles and wilt soon after planting. Further dessication occurs when leaves come in contact with black plastic mulch used in the annual production system. Conventional irrigation practices for the establishment of bare-root transplants of strawberry consist of overhead water application for at least 8 hours/day for 10-14 days after planting. Plant growth regulators (PGRs) have been used to modify the growth characteristics of many plants species. A split-block experiment was implemented at the GCREC-Dover, Dover Fla., to determine the effect of the use Prohexidione-Ca (PC) and IBA [(indole-3) butyric acid] on growth, yield and establishment of strawberry. Main blocks consisted of over head establishment irrigation for 4, 8, and 12 days, and sub-plots consisted of treatments of PC applied in the nursery at a rate of 62.5 mg·L-1 2, 4, or 6 weeks before digging, PC applied in the nursery at 31.25 mg·L-1 2 weeks before digging, a root dip of transplants in 100 mg·L-1 IBA just prior to transplanting. The experiment was conducted for four growing seasons. Data were recorded for marketable yield, number of marketable berries (>10g), and disease incidence. Significant differences were detected for duration of establishment irrigation and growth regulator treatment. No interaction was shown between establishment irrigation and growth regulator treatment.