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- Author or Editor: C. W. Heuser x
- HortScience x
Abstract
Shoots tips from in vitro cultures of Heuchera sanguinea Engelm. ‘Rosamundi’ were cultured on a modified woody plant medium containing one of 3 cytokinins (BA, 2iP, kinetin) or cytokinin plus NAA. BA and kinetin were the most effective stimulators of axillary shoot growth. Maximum shoot production (≥1 mm) occurred with 2, 4, 8, and 16 μm BA, or 16, 32, and 64 μm kinetin. The addition of NAA had no effect or reduced the number of shoots produced. Because of the rosette growth pattern, rooting of the resultant shoots was best accomplished by placing whole cultures in a soilless medium under high humidity and later dividing the rooted shoot masses. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP); N-(2-furanylmethyl)-1H-purin-6-amine (kinetin); and 1-naphthaleneacetic acid (NAA).
Abstract
Single node explants from in vitro derived shoots of Vinca minor L. ‘Bowlesii’ were cultured on modified woody plant medium (WPM). A more effective promoter of axillary shoot proliferation was 6-benzylamino purine (BA) than 6-furfurylamino purine (kinetin) or 6-(γ,γ-dimethylallylamino) purine (2iP). Maximum production of shoots 5 mm and longer occurred in a medium containing 64 μm BA and 0.1 μm α naphthaleneacetic acid (NAA), whereas 64 μm BA and 1.0 μm NAA produced the most shoots 10 mm and longer. Proliferated shoots were rooted and established in a soilless medium under high humidity.
Abstract
Shoot tips from in vitro cultures of Veronica spicata L. ‘Red Fox’ were grown on modified Woody Plant Medium containing one of 3 cytokinins or cytokinin plus NAA. Of the 3 cytokinins tested (BA, kinetin, and 2iP), BA was the most effective stimulator of axillary shoot growth. The greatest number of shoots greater than or equal to 5 mm in length was produced on medium containing 8 μM BA plus 0.01 μM NAA, whereas medium containing 2 μM BA plus 0.01 μM NAA produced the greatest number of shoots greater than or equal to 10 mm. Shoots were rooted and established at an 80% rate in a soilless medium under high humidity. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP); N-(2-furanylmethyl)-1H-purin-6-amine (kinetin); 1-naphthaleneacetic acid (NAA).