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- Author or Editor: Boshou Liao x
- HortScience x
In this study, the pod development dynamics and culture response in lima bean (Phaselous lunatus L.) were investigated. The average percentage of flowers to form pins was 17.5%. Pin length and width (mm), and weight (g) were all highly correlated with the days after pollination (DAP), with correlation coefficients of 0.98, 0.99 and 0.97, respectively. Pods grew relatively faster between 12 and 20 DAP, and reached their maximum length at about 35 DAP. Explants from pods of 5, 10, 15, 20, 25 DAP were cultured onto B5 medium containing BA (2 mg/L), kinetin (0.5 mg/L), 2,4-D (1 mg/L) or NAA (1 mg/L), sucrose (5%), and agar (0.7%). Soft calli only formed from the cut region on the seed coat or the suspensor attachment site of 5 and 10 DAP seeds. The 15 DAP explants were cultured as embryos (cotyledons 2 mm in length), and no callus was observed on them after 30 days of culture when they became brown. Twenty and 25 DAP embryos initiated calli and/or organ-like structures on the abaxial surface of cotyledons or embryo axes after 20 days of culture.