This study investigated the preharvest carbohydrate status and postharvest ethylene action of unrooted shoot-tip cuttings of lantana ‘Dallas Red’ harvested at three times during the day (0800, 1200, and 1600 hr) in relation to subsequent leaf abscission, shoot apices blackening, and adventitious root formation. The cuttings harvested at various times during the day were stored in darkness at 20 ± 1 °C for 4 days in sealed polyethylene bags. The cuttings harvested at 0800 hr had lowest total nonstructural carbohydrate concentrations; however, the amount of ethylene production during postharvest storage was similar among harvest times and increased during the storage period. After 4 days of storage, 69% of the leaves of cuttings harvested at 0800 hr abscised, but only 22% and 8% of the leaves abscised in cuttings harvested at 1200 and 1600 hr, respectively. Application of 1-methylcyclopropene (1-MCP) increased ethylene production and suppressed leaf abscission regardless of the harvest time, but cuttings harvested at 0800 hr developed blackened shoot apices. Leaf abscission was negatively correlated with total nonstructural carbohydrate concentration in the leaves, but no relationship was found with ethylene production. These results indicate that a high endogenous carbohydrate status decreases the postharvest ethylene sensitivity in unrooted shoot-tip cuttings of lantana. Time of harvest influenced subsequent rooting response; however, 1-MCP application did not inhibit rooting. Among various storage treatments, the best rooting response was observed in cuttings harvested at 1600 hr and treated with 1-MCP. Therefore, significant improvement of postharvest storage quality in vegetative lantana cuttings could be achieved by harvesting cuttings late in the day and treating with 1-MCP.
Vijaya Kumar Rapaka, James E. Faust, John M. Dole and Erik S. Runkle
W. Roland Leatherwood, John M. Dole, Ben A. Bergmann and James E. Faust
Knowing which herbaceous taxa are ethylene sensitive and managing exposure of unrooted terminal stem cuttings to ethylene in those taxa are critical for maintaining high-quality propagules that root readily. Of 59 taxa surveyed, freshly harvested terminal cuttings of Begonia hybrid ‘Snowcap’, Lantana camara L. ‘Patriot Sunbeam’, and Portulaca oleracea L. ‘Fairytales Sleeping Beauty’ were sensitive to exogenous application of 1 μL·L−1 ethylene, as demonstrated by leaf abscission within 24 hours of treatment. Exposure to 1-methylcyclopropene (1-MCP) at 700 μL·L−1 for 4 hours before ethylene treatment prevented ethylene injury in these species/cultivars. Exposing unrooted cuttings to 700 μL·L−1 1-MCP induced significant endogenous ethylene biosynthesis in terminal cuttings of the five taxa tested: Euphorbia pulcherrima Willd. ex Klotzsch ‘Visions of Grandeur’, Impatiens hawkeri W. Bull ‘Sonic Red’, Pelargonium peltatum (L.) L’Hérit. ‘Mandarin’, Pelargonium ×hortorum Bailey (pro sp.) [inquinans × zonale] ‘Rocky Mountain White’, and Petunia ×hybrida Vilm. ‘Suncatcher Coral Prism’. Exogenous 1 μL·L−1 ethylene improved adventitious rooting in two cultivars: Begonia hybrid Anita Louise and Fuchsia triphylla L. Honeysuckle. Other trials showed that 1-MCP exposure reduced root number and length of P. ×hortorum ‘Kardino’ and delayed adventitious rooting in all six cultivars tested: Angelonia angustifolia Benth. ‘Carita Lavender’, Calibrachoa ×hybrida Llave & Lex. ‘Terra Cotta’, I. hawkeri ‘Sonic Red’, P. oleracea ‘Fairytales Sleeping Beauty’, Sutera cordata Kuntze ‘Abunda Blue Improved’, and Verbena ×hybrida Groenl. & Ruempl. ‘Aztec Wild Rose’. Subsequent exposure to 1 μL·L−1 ethylene partially mitigated the negative effects on rooting from exposing cuttings to 1-MCP.
Brian A. Krug, Brian E. Whipker, Ingram McCall and John M. Dole
Preplant bulb soaks of flurprimidol, paclobutrazol, and uniconazole; foliar sprays of ethephon and flurprimidol; and substrate drenches of flurprimidol were compared for height control of `Anna Marie' hyacinths (Hyacinthus orientalis). Preplant bulb soak concentrations of flurprimidol and paclobutrazol were from 25 to 400 mg·L-1, and uniconazole from 5 to 80 mg·L-1. Height control was evaluated at anthesis and 11 days later under postharvest conditions. Ethephon (250 to 2000 mg·L-1) and flurprimidol (5 to 80 mg·L-1) foliar sprays were ineffective. Flurprimidol (0.25 to 4 mg/pot) drenches had no effect during forcing, but controlled postharvest height at concentrations ≥0.25 mg/pot a.i. with at least 4% shorter plants than the untreated control. Preplant bulb soaks resulted in height control with flurprimidol ≥25 mg·L-1, paclobutrazol ≥100 mg·L-1, and uniconazole ≥40 mg·L-1; having at least 9%, 6%, and 19%, respectively, shorter plants than the untreated control. Based on our results, flurprimidol preplant bulb soaks have a greater efficacy than either uniconazole or paclobutrazol. Preplant PGR soaks are a cost-effective method of controlling plant height of hyacinths because of the limited amount of chemical required to treat a large quantity of bulbs.
Erin M.R. Clark, John M. Dole, Alicain S. Carlson, Erin P. Moody, Ingram F. McCall, Frankie L. Fanelli and William C. Fonteno
Each year a wide variety of new cultivars and species are evaluated in the National Cut Flower Trial Programs administered by North Carolina State University and the Association of Specialty Cut Flower Growers. Stems of promising and productive cultivars from the National Trial Program were pretreated with either a commercial hydrating solution or deionized (DI) water and placed in either a commercial holding solution or DI water. Over 8 years, the vase life of 121 cultivars representing 47 cut flower genera was determined. Although there was cultivar variation within each genus, patterns of postharvest responses have emerged. The largest category, with 53 cultivars, was one in which a holding preservative increased vase life of the following genera and species: acidanthera (Gladiolus murielae), basil (Ocimum basilicum), bee balm (Monarda hybrid), black-eyed susan (Rudbeckia hybrids), campanula (Campanula species), celosia (Celosia argentea), common ninebark (Physocarpus opulifolius), coneflower (Echinacea purpurea), coral bells (Heuchera hybrids), feverfew (Tanacetum parthenium), foxglove (Digitalis purpurea), ladybells (Adenophora hybrid), lisianthus (Eustoma grandiflorum), lobelia (Lobelia hybrids), obedient plant (Physostegia virginiana), ornamental pepper (Capsicum annuum), pincushion flower (Scabiosa atropurpurea), pinkflower (Indigofera amblyantha), seven-sons flower (Heptacodium miconioides), shasta daisy (Leucanthemum superbum), sunflower (Helianthus annuus), snapdragon (Antirrhinum majus), sweet william (Dianthus hybrids), trachelium (Trachelium caeruleum), and zinnia (Zinnia elegans). Hydrating preservatives increased the vase life of four basils, coral bells, and sunflower cultivars. The combined use of hydrator and holding preservatives increased the vase life of three black-eyed susan, seven-sons flower, and sunflower cultivars. Holding preservatives reduced the vase life of 14 cultivars of the following genera and species: ageratum (Ageratum houstonianum), false queen anne's lace (Ammi species), knotweed (Persicaria hybrid), lisianthus, pineapple lily (Eucomis comosa), sneezeweed (Helenium autumnale), yarrow (Achillea millifolium), and zinnia. Hydrating preservatives reduced the vase life of 18 cultivars of the following genera and species: feverfew, lisianthus, ornamental pepper, pineapple lily, seven-sons flower, shasta daisy, sneezeweed, sweet william, sunflower, trachelium, yarrow, and zinnia. The combined use of hydrating and holding preservatives reduced the vase life of 12 cultivars in the following genera and species: false queen anne's lace, feverfew, pincushion flower, sneezeweed, sunflower, trachelium, yarrow, and zinnia. Data for the remaining 50 cultivars were not significant among the treatments; these genera and species included beautyberry (Callicarpa americana), black-eyed susan, blue mist (Caryopteris clandonensis), calendula (Calendula officinalis), campanula, cleome (Cleome hasserliana), common ninebark, dahlia (Dahlia hybrids), delphinium (Delphinium hybrids), flowering peach (Prunus persica forma versicolor), heliopsis (Heliopsis helianthoides), hemp agrimony (Eupatorium cannabinum), himalayan honeysuckle (Leycesteria formosa), hydrangea (Hydrangea paniculata), larkspur (Consolida hybrids), lily of the nile (Agapanthus hybrid), lisianthus, lobelia, ornamental pepper, pineapple lily, scented geranium (Pelargonium hybrid), sunflower, sweet william, and zinnia.