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  • Author or Editor: R. J. Henny x
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Abstract

Aphelandra squarrosa Nees. plants grown under 250 μmol s−lm−2 photosynthetically active radiation (PAR) were transferred to 265, 737, and 1070 μmol s−1m−2. Stomatal density and number of palisade layers of newly expanded leaves decreased linearly after 2 months, while number of countable chloroplasts per palisade cell increased linearly as light intensity decreased. Number of countable chloroplasts per palisade cell decreased and palisade layers increased in mature leaves transferred to higher PAR levels. Stomatal density and guard cell length in mature leaves did not change. There were nonlinear responses to PAR levels in number of marginal collenchyma cells, leaf thickness, and palisade cell length of immature leaves. Chloroplasts in mature and immature leaves were less discrete and more tightly appressed to anticlinal palisade cell walls as PAR levels increased.

Open Access

Randomly amplified polymorphic DNA (RAPD) markers were utilized to determine the genetic relationships of nine morphologically similar pot plant cultivars of Anthurium sp. by developing DNA fingerprints (DFP). Of 25 arbitrary primers screened, nine generated DFPs that were used in computing the genetic distance (d) and similarity coefficient (C) values. All cultivars tested exhibited a high degree of genetic similarity. `Lady Ann' and `Lady Beth' possessed the closest relationship with d and C values of 0.06 and 0.98, respectively. The next closest genetic relationship was between `Red Hot' and `Southern Blush' (d = 0.33, C = 0.89). These two cultivars exhibited a more distant relationship to the other seven cultivars as indicated by higher `d' values. However, this study showed that the nine Anthurium cultivars examined were genetically closely related. These cultivars share specific DNA bands with three possible parental species (A. andraeanum Linden ex Andre, A. antioquens L., and A. amnicola Dressler) included in this study, which may indicate similarities in their pedigree. This study shows that RAPDs can be a useful tool to distinguish Anthurium pot plant cultivars as well as identify their genetic relationships.

Free access