To determine the nutrient solution copper (Cu2+) level above which Cucumis sativus L. (cucumber, cv. LOGICA F1) plant growth and fruit yield will be negatively affected, plants were grown on rockwool and irrigated with nutrient solutions containing Cu2+ at 0.05, 0.55, 1.05, 1.55, and 2.05 mg·L−1. Copper treatment began when plants were 4 weeks old and lasted for 10 weeks. During this 10-week period, plants were harvested at 3 weeks (short-term) and 10 weeks (long-term) after the start of Cu2+ treatment. Neither visible leaf injury nor negative Cu2+ effect was observed on plant growth (leaf number, leaf area, leaf dry weight, and stem dry weight) after 3 weeks of continuous Cu2+ treatment. However, after 10 weeks of continuous Cu2+ application, cucumber leaf dry weight was significantly reduced by Cu2+ levels 1.05 mg·L−1 or greater; leaf number, leaf area, and stem dry weight were significantly reduced by Cu2+ levels 1.55 mg·L−1 or greater. Copper (Cu2+ levels 1.05 mg·L−1 or greater) also caused root browning. Some plants under the 2.05 mg·L−1 Cu2+ treatment started to wilt after 6 weeks of continuous Cu2+ treatment. Copper treatment did not result in any change in leaf greenness until after Week 9 from the start of the treatments. There was no sign of a negative Cu2+ effect on cucumber fruit numbers after the first 2 weeks of production, but plants under the highest Cu2+ concentration treatment (2.05 mg·L−1) gradually produced fewer cucumber fruit than the control (0.05 mg·L−1) and eventually resulted in lower cucumber yield. Nutrient solution can be treated with 1.05 mg·L−1 of Cu2+ in cucumber production greenhouses; however, it is not recommended to use Cu2+ concentrations 1.05 mg·L−1 or greater continuously long-term (more than 3 weeks). When applying Cu2+, it is suggested that cucumber roots be examined regularly because roots are a better indicator for Cu2+ toxicity than leaf injury.
Youbin Zheng, Linping Wang, Diane Feliciano Cayanan and Mike Dixon
Diane Feliciano Cayanan, Mike Dixon, Youbin Zheng and Jennifer Llewellyn
The recycling of irrigation water may cause the dispersal of plant pathogens. Irrigation water disinfected with 2.4 mg·L−1 of free chlorine for 5 min was overhead-applied to 17 container-grown nursery plants for 11 weeks in a commercial nursery to evaluate the response of container-grown nursery plants to chlorine. No visual symptoms of injury or growth reduction were observed on the evergreen shrubs (Juniperus horizontalis, Thuja occidentalis, Buxus microphylla, Picea glauca, Rhododendron catawbiense, Taxus media, and Chamaecyparis pisifera), but there were visual injuries and/or growth reduction on some of the deciduous shrubs (Salix integra, Hydrangea paniculata, Prunus ×cistena, Weigela florida, Physocarpus opulifolius). Symptoms of anthracnose were reduced on Cornus alba plants treated with chlorinated water. The chlorine treatment did not affect leaf chlorophyll content. The chlorine treatment killed all fungi and oomycetes in the irrigation water (DNA multiscan). Although there were visible leaf injuries and growth reduction on some of the deciduous plants, chlorine injury did not render them unsalable. Results suggest that irrigation water treated with 2.4 mg·L−1 free chlorine for 5 min will effectively control the dispersal of common plant pathogens without reducing the market value of container-grown plants.
Thomas Graham, Ping Zhang, Youbin Zheng and Michael A. Dixon
The phytotoxic threshold of five woody perennial nursery crops to applications of aqueous ozone was investigated to determine if aqueous ozone could be used for remediation of recycled nursery irrigation water and for pathogen control. The perennial nursery crops [Salix integra Thunb. ‘Hakura Nishiki’; Weigela florida Thunb. ‘Alexandra’; Spiraea japonica L.f. ‘Goldmound’; Hydrangea paniculata Seib. ‘Grandiflora’; Physocarpus opulifolius L. Maxim. ‘Summer Wine’] were evaluated for aqueous ozone phytotoxicity after 6 weeks of overhead spray irrigation in which five aqueous ozone treatments (0, 10.4, 31.2, 62.5, 125.0 μmol·L−1) were applied on a daily basis. The concentrations applied represent levels useful for irrigation system maintenance (pathogen and biofilm control) with the highest levels selected to clearly demonstrate phytotoxicity. Aqueous ozone solutions were prepared and injected in-line during irrigation for 7.5 min every day for 6 weeks, after which growth parameters (leaf area, shoot dry weight, root dry weight, height, flower number) were measured and leaf injury was evaluated. High residual aqueous ozone (62.5 μmol·L−1 or greater at emitter discharge; 0.3 m from canopy) in the irrigation water was shown to negatively affect the growth parameters measured; however, low residual ozone concentrations (31.2 μmol·L−1 or less at emitter discharge; 0.3 m from canopy) did not present any measurable risk to plant growth. Furthermore, even at higher dose levels, leaves produced during the treatment period showed reduced damage levels. It is concluded that ozone residuals of 31.2 μmol·L−1 (at emitter discharge) can remain in overhead irrigation water without negatively affecting the crop species examined under the application protocols used. At the ozone concentrations demonstrated to be tolerable by the crop species examined, it is reasonable to surmise that control of pathogens at all points within the irrigation system will be achievable using aqueous ozone as part of an irrigation management strategy. The use of aqueous ozone in this fashion could also aid in dramatically reducing chemical residuals on crops by reducing the input requirements of traditional chemical controls.
Diane Feliciano Cayanan, Ping Zhang, Weizhong Liu, Mike Dixon and Youbin Zheng
Recycled irrigation water is one of the major sources of inoculum and may spread plant pathogens throughout the nursery or greenhouse operation. Chlorination is the most economical method of disinfecting water and has been adopted by some North American commercial growers. However, chlorine has not been assessed as a disinfectant for the common plant pathogens Phytophthora infestans, Phytophthora cactorum, Pythium aphanidermatum, Fusarium oxysporum, and Rhizoctonia solani. These pathogens were exposed to five different initially free chlorine solution concentrations ranging from 0.3 to 14 mg·L−1 in combination with five contact times of 0.5, 1.5, 3, 6, and 10 min to determine the free chlorine threshold and critical contact time required to kill each pathogen. Results indicated that the free chlorine threshold and critical contact time for control of P. infestans, P. cactorum, P. aphanidermatum, F. oxysporum, and R. solani were 1, 0.3, 2, 14, and 12 mg·L−1 for 3, 6, 3, 6, and 10 min, respectively.
Diane Feliciano Cayanan, Youbin Zheng, Ping Zhang, Tom Graham, Mike Dixon, Calvin Chong and Jennifer Llewellyn
Phytotoxic responses of five container-grown nursery species (Spiraea japonica ‘Goldmound’, Hydrangea paniculata ‘Grandiflora’, Weigela florida ‘Alexandra’, Physocarpus opulifolius ‘Summer Wine’, and Salix integra ‘Hakura Nishiki’) to chlorinated irrigation water and critical free chlorine thresholds were evaluated. Plants were overhead-irrigated with water containing 0, 2.5, 5, 10, and 20 mg·L−1 of free chlorine for 6 weeks. The following measurements were used to assess the treatments: visual injury, growth, leaf chlorophyll content index, leaf chlorophyll fluorescence, leaf net CO2 exchange rate, and stomatal conductance. All species exhibited one or more signs of chlorine injury, including foliar necrotic mottling, foliar necrosis and chlorosis, decreased plant height, and increased premature abscission of foliage with species varying in sensitivity to free chlorine concentrations of irrigation water. The results indicated that the critical free chlorine threshold of S. japonica, H. paniculata, W. florida, and S. integra was 2.5 mg·L−1 and 5 mg·L−1 for P. opulifolius. Our results suggested that irrigation water containing free chlorine less than 2.5 mg·L−1 should not adversely affect the growth or appearance of ornamental woody shrubs.
Yun Kong, David Llewellyn, Katherine Schiestel, Martha Gay Scroggins, David Lubitz, Mary Ruth McDonald, Rene Van Acker, Ralph C. Martin, Youbin Zheng and Evan Elford
There is a potentially large market for locally produced organic bitter melons (Momordica charantia L.) in Canada, but it is a great challenge to grow this warm-season crop in open fields (OFs) due to the cool and short growing season. To test the feasibility of using high tunnels (HTs) for organic production of bitter melons in southern Ontario, plant growth, fruit yield and quality, and pest and disease incidence were compared among three production systems: OF, HT, and high tunnel with anti-insect netting (HTN) at Guelph in 2015. The highest marketable fruit yield was achieved in HTN (≈36 t·ha−1), followed by HT (≈29 t·ha−1), with the lowest yield obtained in OF (≈3 t·ha−1). Compared with OF, there were several other benefits for bitter melon production in HT and HTN: increased plant growth, advanced harvest timing, reduced pest numbers and disease incidence, and improved fruit quality traits such as increased individual fruit weight and size, and reduced postharvest water loss. In addition to higher yield, HTN had fewer insect pests and disease incidence compared with HT. The results suggest that HTs can be used for organic production of bitter melon in southern Ontario and regions with similar climates. Also, the addition of anti-insect netting to HTs is beneficial to production if combined with an effective pollination strategy.