The feasibility of using RFLP to distinguish genetically related Hybrid Tea rose cultivars for DNA `fingerprinting' was examined with a group of cultivars related to `Peace'. The following cultivars used in this study, `Chicago Peace', `Flaming Peace', `Climbing Peace' and `Lucky Piece', were derived from bud mutations (sports) of `Peace'. We also investigated two additional cultivars, `Perfume Delight' and `Garden Party', in which one of the parents for each was `Peace'. Genomic rose DNA probes, cloned in pUC8 plasmid of Escherichia coli, were hybridized with genomic DNA of these cultivars digested with different restriction enzymes. Although polymorphisms were observed among these related cultivars, only a few probe/enzyme combinations screened produced RFLPs due to the high degree of genetic relatedness of these cultivars. We have identified probes that can distinguish all of these related rose cultivars. This study demonstrates that RFLP markers can be used effectively in DNA `fingerprinting' of genetically related rose cultivars, eventhough the level of detectable polymorphism is quite low.
Sriyani Rajapakse, Albert Abbott, John Kelly and Robert Ballard
Marshall K. Elson, John F. Kelly and Muraleedharan G. Nair
Actinomycetes were isolated from asparagus field soil and bioassayed against Fusarium spp. in petri dishes. Extracts of the active organisms were bioassayed to determine if inhibition was caused by competition or antibiosis. The most active, antibiotic-producing organism was inoculated into test tubes with asparagus and Fusarium and evaluated for disease control. Asparagus seedlings were dipped in actinomycete suspension before planting in Fusarium-infested soil. These seedlings were evaluated for disease incidence after 8 weeks. Asparagus crowns could be dipped in actinomycete suspension before planting in the field.
Shaun F. Kelly, J.L Green and John S. Selker
Time Domain Reflectometry (TDR) is used to measure in situ soil moisture content and salinity of porous media. Commercially available TDR systems used for field measurements have limited use in laboratory scale experiments where short high resolution probes are needed. A short TDR probe was designed for use with high bandwidth TDR instruments currently available. The probes are designed from SMA bulkhead connectors using gold-plated stainless steel wire 0.035 inches in diameter. A 20.GHz digital sampling oscilloscope (11801; Tektronix, Beaverton, Ore.) with an SD-24 TDR sampling head is used with the probes to determine water content and ion concentrations in porous media. The 7.5- and 3.0-cm-long probes were used to measure soil moisture content and ion concentrations in laboratory columns. Fertilizer and water gradients were observed by using bromide salts brought into contact with the top of laboratory columns, 7.6 cm in diameter and 18 cm long, packed with container media [1 peat: 1 vermiculite v/v)]. Soil moisture measurements in the presence of high concentrations of salts were made by insulating the probes with Teflon heat-shrinkable tubing to minimize conductivity losses.
Lisa Chen Cushman, H. Brent Pemberton and John W. Kelly
Experiments were conducted to study the interaction of cultivar, flower stage, silver thiosulfate (STS), and BA on flower senescence and leaf abscission in greenhouse-grown potted miniature roses. Plants of Rosa L. `Meijikatar' (Orange Sunblaze) and `Meirutral' (Red Sunblaze) were sprayed with several concentrations of STS and BA in factorial combination. In winter, plants were sprayed with STS at 0 or 2 mm and BA at 0, 0.02,0.04,0.11,0.22, or 0.44 mm In spring, flowers at three stages of development were sprayed with STS at 0,2, or 3 mm, and BA at 0, 0.02, 0.04, 0.22, or 0.44 mm One day after treatment in both experiments, plants were placed in darkness at 16C for 4 days to simulate shipping, and then they were evaluated in a controlled environment at 21C. Poststorage floral longevity (PSFL) was longer for `Meirutral' than for `Meijikatar' plants, regardless of chemical treatment or flower stage. Flowers that were in the bud stage (stage 1) before simulated shipping lasted longer than flowers showing color (stages 2 and 3), regardless of cultivar or chemical treatment. Combinations of STS and BA did not increase PSFL compared to STS alone. Plants treated with 2 or 3 mm STS exhibited longer PSFL than nontreated plants; however, 2 and 3 mm were about equally effective. STS at 4 mm was phytotoxic in a preliminary experiment. Applying BA alone did not affect PSFL, but did improve postharvest flower opening on `Meijikatar' plants about the same as STS applied alone. The large flowering cultivars represented by `Meijikatar' and `Meirutral' appear to be nonresponsive to BA. A star-shaped malformation was induced on `Meijikatar' and `Meirutral' plants by simulated shipping and was not prevented by STS or BA. Chemical name used: N-(phenylmethyl) -1H-purin-6-amine (BA).
Katherine Kelly Stephenson, John R. Stommel and Timothy J Ng
A protocol was developed to make in vitro graft unions among Lycopercicon spp., and regenerates from cultured graft unions were evaluated for chimera formation. Young seedlings were preconditioned for 4 to 6 days in liquid 1/2-strength Murashige & Skoog (MS) basal medium supplemented with 8.9 μM benzyladenine and 1.0 μM indole-3-butyric acid. Preconditioned seedlings exhibited increased biomass and enhanced graft union survival. In particular, survival of cleft grafts increased from 37% to 95% with the seedling preconditioning. When graft unions among different genotypes were excised from apex-to-apex in vitro cleft grafts and plated on MS basal medium supplemented with 9.1 μM zeatin and 3.9 μM ancymidol, as many as 100 plantlets were regenerated from a single graft union. However, no chimeric regenerates were recovered, indicating that asymmetric responses to grafting may be a limiting factor to in vitro chimera formation.
Robert J. Dufault, Tyron L. Phillip and John W. Kelly
Gerbera seedlings (Gerbera jamesonii H. Bolus Ex. Hook F.) `Florist Strain Yellow' were planted on drip-irrigated, plastic-mulched beds at 24,000, 36,000 or 72,000 plants/ha. Nitrogen and potassium fertilizers at 55, 110, or 220 kg·ha-1 were factorially combined with populations. In the 1st year of a 2-year study, the number of marketable flowers increased as N and K increased to 110 kg·ha-1, but as N and K were increased to 220 kg·ha-1, cull production increased. In the 2nd year, marketable and cull yields increased with N rate to 220 kg·ha-1; K did not affect yield. As populations increased from 24,000 to 72,000 plants/ha, marketable and cull flower production increased in both years. Flower size and quality were unaffected by plant populations. Nitrogen and potassium fertility did not affect flower size, quality, or vase life in either year.
Nihal C. Rajapakse, David Wm. Reed and John W. Kelly
Experiments were conducted to evaluate Dendranthema × grandiflorum (Ramat.) Kitamura cv. Bright Golden Anne quality and post-storage growth following storage in the range of 5 to 35C, initial soil water levels (60%, 80%, 100%), and durations (0 to 8 days). Transpiration rate showed a quadratic relationship with storage temperature. Initial soil water content had little effect on transpiration rate in dark storage environments. The lowest transpiration rate was observed in plants stored at 15 or 20C. Amino acid (AA) leakage and post-storage growth were well-correlated. Plants stored at or above 25C became etiolated during storage, while storage at 15C or below did not cause etiolation. Temperatures at or below 15C did not affect subsequent growth rate of chrysanthemum plants. Storage at 20C and above caused a reduction in post-storage growth rate following 2 days of storage.
Shaun F. Kelly, James L. Green and John S. Selker
The process of fertilizer diffusion was examined using KBr and NaBr salts placed at the top of columns filled with a container medium at an initial water content of 4.0, 2.5, or 1.0 g·g-1 (mass of water/mass of medium). Columns were sealed to create a protected diffusion zone (PDZ) shielding the system from water infiltration and evaporation. Bromide and water distributions were determined after 5, 10, 25, and 120 days. Using a Fickian diffusion model, effective diffusion coefficients calculated for Br- in the medium at 2.5 g·g-1 ranged from 2.7 to 4.6 × 10-6 cm2·s-1, which is 3 to 9 times less than the diffusion coefficient in water alone. Diffusion rates increased with increasing medium water content. Differences in the hygroscopicity and solubility of KBr and NaBr affected the distribution of water and diffusion rates in the columns. Redistribution of water was driven to a significant degree by vapor-phase transport, caused by large gradients in osmotic potential, and was most apparent at low water content. At high water content, water redistribution was affected by solution density gradients in the system. This significantly complicates the mathematical modeling of the system, which renders a simple Fickian diffusion model of limited predictive value in high and low water content media.
Nihal C. Rajapakse, William B. Miller and John W. Kelly
Low-temperature storage potential of rooted cuttings of garden chrysanthemum [Dendranthema ×grandiflorum (Ramat.) Kitamura] cultivars and its relationship with carbohydrate reserves were evaluated. Storage of chrysanthemum cuttings at -1 and -3 °C resulted in freezing damage. Visual quality of rooted cuttings stored at 0 or 3 °C varied among cultivars. Quality of `Emily' and `Naomi' cuttings was reduced within a week by dark storage at 0 or 3 °C due to leaf necrosis, while `Anna' and `Debonair' cuttings could be held for 4 to 6 weeks without significant quality loss. In `Anna' and `Debonair', low-temperature storage reduced the number of days from planting to anthesis regardless of storage duration. However, flowers of plants grown from stored cuttings were smaller than those of nonstored cuttings. At the beginning of storage, `Emily' and `Naomi' had lower sucrose, glucose, and fructose (soluble sugars) content compared to `Anna' and `Debonair'. Regardless of temperature, leaf soluble sugar was significantly reduced by dark storage for 4 weeks. In stems, sucrose and glucose were reduced while fructose generally increased during low-temperature storage probably due to the breakdown of fructans. Depletion of soluble sugars and a fructan-containing substance during low-temperature dark storage was greater in `Emily' and `Naomi' than in `Anna' and `Debonair'. Low irradiance [about 10 μmol·m-2·s-1 photosynthetically active radiation (PAR) from cool-white fluorescent lamps] in storage greatly improved overall quality and delayed the development of leaf necrosis in `Naomi'. Cuttings stored under light were darker green and had a higher chlorophyll content. Leaf and stem dry weights increased in plants stored under medium and high (25 to 35 μmol·m-2·s-1 PAR) irradiance while no change in dry weight was observed under dark or low light. Results suggest that the low-temperature storage potential of chrysanthemum cultivars varies considerably, and provision of light is beneficial in delaying the development of leaf necrosis and maintaining quality of cultivars with short storage life at low temperatures.
David G. Clark, John W. Kelly and Nihal C. Rajapakse
The effects of carbon dioxide enrichment on growth, photosynthesis, and postharvest characteristics of `Meijikatar' potted roses were determined. Plants were grown in 350, 700, or 1050 μl CO2/liter until they reached 50% flower bud coloration and then were placed into dark storage for 5 days at 4 or 16C. Plants grown in 700 or 1050 μl CO2/liter reached the harvest stage earlier and were taller at harvest than plants produced in 350 μl CO2/liter, but there were no differences in the number of flowers and flower buds per plant among CO2 treatments. Plants grown in early spring were taller and had more flowers and flower buds than plants grown in late winter. Shoot and root growth of plants grown in 700 or 1050 μl CO2/liter were higher than in plants produced in 350 μl CO2/liter, with plants grown in early spring showing greater increases than plants grown in late winter. Immediately after storage, plants grown in 350 μl CO2/liter and stored at 4C had the fewest etiolated shoots, while plants grown in 1050 μl CO2/liter and stored at 16C had the most. Five days after removal from storage, chlorophyll concentration of upper and lower leaves had been reduced by ≈50% from the day of harvest. Carbon dioxide enrichment had no effect on postharvest leaf chlorosis, but plants grown in early spring and stored at 16C had the most leaf chlorosis while plants grown in late winter and stored at 4C had the least leaf chlorosis.