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Many small-scale vegetable growers in the United States who graft their own vegetable transplants use healing chambers inside a greenhouse to heal their grafted plants. Under these conditions, light and relative humidity (RH) can fluctuate during the healing process, and growers need more research-based information regarding the impact of these factors on the survival of grafted transplants. To address this need, this study investigated the effect of different targeted levels of light (0%, 25%, and 50%) and RH (50% and 100%) (six combinations) in a small-scale healing chamber within a greenhouse, where the healing chamber was opened for increasing periods of time for 8 days, at which time plants were fully exposed to greenhouse conditions. The survival and growth of self-grafted eggplant (Solanum melongena), pepper (Capsicum annuum), and tomato (Solanum lycopersicum) were measured up to 25 days post grafting. Percent light in the closed healing chambers was similar for the 50% and 100% RH levels of each light treatment. When the healing chambers were closed, compared with the greenhouse, there was 0.1% light in the 0% light treatments, 25% light on average in the 25% light treatments, and 43% light on average in the 50% light treatments. On days 2 to 5 after grafting, when chambers were opened up to 1 hour, average RH in the healing chambers was 96% to 98% for the 100% RH treatments, and was 42% to 49% for the 50% RH treatments. On days 6 and 7, when chambers were opened for 3 to 8 hours, RH was 79% to 82% for the 100% RH treatments, and was 39% to 46% for the 50% RH treatments. Survival of grafted plants following healing was greatest when the healing chamber treatment was 100% RH and 50% or 25% light (95% and 90% survival, respectively), and plant survival with these two treatments did not significantly decline from 11 to 21 days after grafting, indicating plants were fully healed and acclimated when they were removed from the healing chambers on day 8. At 22 to 25 days following grafting, plants healed with 100% RH and 50% or 25% light had greater plant height, number of leaves per plant, and stem diameter than plants healed in the other light and RH combinations. SPAD reading and nitrate-nitrogen of fresh petiole sap were unaffected by any of the healing treatments tested in this experiment, or by crop type. Tomato and pepper had 14% greater survival rates on average than eggplant at all measurement dates, while tomato tended to have greater plant growth, followed by eggplant and pepper. Additional research is needed to improve survival of grafted eggplant.

‘Brown Snout’ cider apple (Malus ×domestica) is desired by cider makers for its relatively high levels of phenolics, and over-the-row machine harvesting of ‘Brown Snout’ has been demonstrated to provide similar yield to hand harvest at a significantly lower cost. The purpose of this study was to determine if there is a measurable impact of harvest method on the phenolic profile of ‘Brown Snout’ juice and cider to better inform equipment adoption recommendations. Using a redox titration assay, the titratable tannin content (± SE) of juice (0.19% ± 0.01%) and cider (0.19% ± 0.01%) were found not to differ due to harvest method. Using a protein precipitation assay, juice from machine-harvested fruit was found to have lower levels of total tannins [231 ± 36 mg·L⁻¹ catechin equivalents (CE)] than juice from hand-harvested fruit (420 ± 14 mg·L⁻¹ CE). However, the total tannins of cider did not differ due to harvest method, the overall average for machine and hand harvest was 203 ± 22 mg·L⁻¹ CE. The total phenolics of juice and cider did not differ due to harvest method (1415 ± 98 mg·L⁻¹ CE and 1431 ± 73 mg·L⁻¹ CE, respectively). Discriminant analysis based on an average of 33 tentatively identified phenolic compounds, as measured by ultra-high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry, showed no separation due to harvest method in juice or cider. In conclusion, over-the-row machine harvesting of ‘Brown Snout’ resulted in a final product of similar quality at reduced labor costs, and thus shows potential for increasing the commercial sustainability of cider apple operations.

Icebox watermelons first appeared in the United States about 50 years ago. They weigh between 6 and 12 pounds and come in a variety of shapes and colors. With a rising interest in local production, organic produce, and direct marketing, farmers in Washington are looking to diversify crop varieties to meet these demands. Icebox watermelons offer a means of locally producing high-quality watermelons. In 2004 and 2005, we evaluated icebox watermelon varieties to determine which are most suitable for production in our region. In 2004, we evaluated 44 varieties of icebox watermelon and in 2005 we evaluated 100 varieties at WSU Vancouver REU. Varieties were seeded in the greenhouse mid-April and transplanted to the field by mid-June. The greenhouse and field were managed organically. The study design was a randomized complete-block with three replications. Plots were single rows, 6.1 m long, with seven plants per plot. Spacing was 1 m between plants and 3 m between rows. Plants were mulched with black plastic and drip-irrigated 2.5 cm per week. Melons were harvested twice weekly, from mid-August through mid-October. Fruit weight, number, and size were measured, and percentage of soluble solids was measured using a °Brix meter. General eating quality was also evaluated. Summaries of selected varieties will be presented here. To view other varieties included in the trial, see our website, http://agsyst.wsu.edu. Results of this study show significant differences among icebox watermelon varieties in total yield, number of fruit per plot, average fruit weight, number of days to harvest, and percentage of soluble solids. These preliminary findings indicate that more than 80 varieties of icebox watermelon can be grown productively in our region.

Four potentially biodegradable mulch products (BioAgri, BioTelo, WeedGuardPlus, and SB-PLA-10) were evaluated during 2010 in three contrasting regions of the United States (Knoxville, TN; Lubbock, TX; and Mount Vernon, WA) and compared with black plastic mulch and a no-mulch control for durability, weed control, and impact on tomato yield in high tunnel and open field production systems. WeedGuardPlus, BioTelo, and BioAgri had the greatest number of rips, tears, and holes (RTH) and percent visually observed deterioration (PVD) at all three sites (P ≤ 0.05), and values were greater in the open field than high tunnels, likely as a result of high winds and greater solar radiation and rainfall. SB-PLA-10 showed essentially no deterioration at all three sites and was equivalent to black plastic in both high tunnels and the open field. Weed growth at the sites did not differ in high tunnels as compared with the open field (P > 0.05). Weed growth at Knoxville and Mount Vernon was greatest under SB-PLA-10 (P ≤ 0.02), likely as a result of the white, translucent nature of this test product. Tomato yield was greater in the high tunnels than open field at all three sites (P ≤ 0.03), except for total fruit weight at Knoxville (P ≤ 0.53). Total number of tomato fruit and total fruit weight were lowest for bare ground at both Knoxville (150 × 10⁴ fruit/ha and 29 t·ha⁻¹; P ≤ 0.04) and Mount Vernon (44 × 10⁴ fruit/ha and 11 t·ha⁻¹; P ≤ 0.008). At Knoxville, the other mulch treatments were statistically equivalent, whereas at Mount Vernon, BioAgri had among the highest yields (66 × 10⁴ fruit/ha and 16 t·ha⁻¹). There were no differences in tomato yield resulting from mulch type at Lubbock.

Season extension structures like high tunnels make it possible to produce cold-tolerant crops during winter months for both a longer cropping season and a winter market season. The effects of location and planting date on the fresh yield of several cultivars of Asian greens (Brassica rapa L.), lettuce (Lactuca sativa L.), and spinach (Spinacia oleracea L.) were examined at Moscow, ID/Pullman, WA, and Vancouver, WA, a cold temperate climate and a mild marine climate, respectively. In Winter 2005–06, 20 cultivars were evaluated and in Winter 2006–07 a subset of 12 cultivars were evaluated. Location impacted yield, and higher yields overall were attained at Vancouver than at Moscow/Pullman, likely as a result of more consistent, warmer soil and air temperatures as well as increasing irradiance in February and March at Vancouver. Asian green cultivars had the highest overall yield resulting from faster growth compared with spinach and lettuce cultivars at both locations. Although most lettuce cultivars grew throughout the winter, further research is needed to identify the most suitable cultivars, seeding dates, and planting densities to optimize winter production of this crop and for Asian greens and spinach. Planting date influenced yields with the highest yields obtained for the third planting date for all trials except at Moscow/Pullman in the second year. Overall, this research suggests that it is possible to grow many cold-tolerant cultivars of Asian greens, spinach, and lettuce in a high tunnel during the winter months in both mild and cold temperate northern climates.

Day-neutral strawberry (Fragaria ×ananassa) is typically grown in plasticulture production systems that use black polyethylene (PE) mulch for weed management and promotion of crop growth and yield. The objectives of this research were to evaluate several commercial plastic and paper biodegradable mulch (BDM) products [Bio360, Experimental Prototype (Exp. Prototype), and WeedGuardPlus] in comparison with standard black PE mulch and bare ground cultivation in day-neutral strawberry grown in an annual system in northwestern Washington. Mulch performance [as percent visual cover (PVC)], weed suppression, marketable yield, plant biomass, and fruit quality were evaluated in ‘Albion’ and ‘Seascape’ strawberry grown in 2014 and 2015. PVC measured at the end of the production season was lowest for the Exp. Prototype (8%) in 2014 and was greatest for Bio360 (90%), WeedGuardPlus (90%), and PE (98%). In 2015, PVC at the end of the production season was again lowest for Exp. Prototype (62%), followed by WeedGuardPlus (64%), Bio360 (93%), and PE mulch (97%). Overall, weed pressure was higher in 2015 relative to 2014 and was greatest in the bare ground treatment in both years of the study. By the end of the 2015 season, weed cover in the bare ground treatment was 95%, followed by WeedGuardPlus (50%), Exp. Prototype (34%), PE (25%), and Bio360 (15%). Yield showed year and cultivar effects and was higher in mulched treatments. Plant biomass showed varying effects; root biomass was lowest in ‘Seascape’ in 2015 under the bare ground treatment and greatest under Bio360, which was similar to PE mulch and WeedGuardPlus. Leaf biomass was lowest in the bare ground treatment and highest in mulched treatments (except in 2015, when leaf biomass was intermediate for plants grown with WeedGuardPlus). Crown biomass showed a similar trend and was overall greater for plants grown in mulched treatments except for Bio360 in 2014, which was the same as the bare ground treatment. Overall, fruit quality was maintained among strawberry grown with BDMs, with soluble solids concentration (SSC, %) and titratable acidity (TA) being the only variables to show treatment effects. SCC tended to be lower in fruit from bare ground plots. TA was different for ‘Seascape’ in 2015 with fruit from bare ground and Exp. Prototype treatments having higher TA than the PE treatment. This study demonstrates that BDMs can be comparable to PE mulch in terms of performance and impacts on crop productivity in day-neutral strawberry, suggesting that BDMs could be a viable alternative to PE mulch for strawberry growers in the Pacific Northwest.

Irrigation water productivity is a priority for sustainable orchard management as water resources become more limiting. This study evaluated reduced irrigation (RI) as a management strategy for cider apple (Malus domestica Borkh.) production in 2019 and 2020 in northwestern Washington, which has a Mediterranean climate and averages 14.1 cm of precipitation from June to September. RI was evaluated on three cider apple cultivars, Dabinett, Porter’s Perfection, and Golden Russet, in their third and fourth leaf. Stem water potential (stem ψ) was measured weekly throughout the growing season to monitor water stress and implement the RI treatment: irrigation would be applied when stem ψ values dropped below −1.5 MPa, a threshold indicative of moderate water stress in apples. Soil water potential was monitored throughout the season, vegetative growth was assessed by measuring shoot length and non-destructive imaging of the plant canopy using lateral photography, and yield, fruit quality, and juice quality were measured at harvest. Moderate water stress as indicated by stem ψ did not occur either year, thus irrigation was never applied to the RI treatment plots. There was a negative relationship between average stem ψ and both yield and air temperature (P < 0.0001 for each); as yield increased by 5.9 kg per tree or temperature increased by 3.3 °C, stem ψ decreased by 0.1 MPa. The juice quality attributes of the three cultivars in this study were similar to their historic measures at this site and there were no differences due to irrigation treatment, likely because trees did not reach the threshold to induce physiological stress. Both years, trees in the RI treatment did not differ from the control treatment in vegetative growth, fruit yield, juice yield, or any juice quality attribute, but weight per fruit decreased by 7 g, and fruit firmness (measured only in 2020) increased by 2 N. Results from this study indicate that fruit yield and quality in an establishing orchard can be maintained when irrigation is reduced relative to crop water requirements that are estimated from a calculated water balance or relative to conventional grower practices for this region. This finding highlights the benefits of using plant water status to schedule irrigation.

Leafy green vegetables such as lettuce (Lactuca sativa), Asian greens (Brassica spp.) and spinach (Spinacia oleracea) have a tendency to accumulate high concentrations of potentially harmful nitrate–nitrogen (NO₃-N). It would be advantageous for growers to have rapid and inexpensive methods to accurately measure plant tissue NO₃-N to make fertility and harvest management decisions for these crops. This study compared fresh sap expressed from whole leaves and analyzed with a Cardy meter with the analysis of dry leaf tissue extracts analyzed with a benchtop ion selective electrode (ISE) and an automated colorimetric method for determining NO₃-N concentration. Results from ISE and colorimetric analysis of the same dry leaf tissue extracts had a strong relationship (r ² = 0.92). The ISE was relatively easy to operate and affordable, suggesting it is an adequate substitute for automated colorimetric analysis of dry plant tissue extracts. Results of fresh whole leaf sap analyzed with the Cardy meter showed a poor relationship with dry leaf tissue extracted and analyzed using the ISE (r ² = 0.25) or with colorimetric analysis (r ² = 0.21). When fresh whole leaf sap was diluted 1:1 with aluminum sulfate [Al₂(SO₄)₃] to adjust for potential matrix effects, there was still a relatively poor relationship (r ² = 0.41) between the diluted sap samples analyzed with a Cardy meter and the dry leaf tissue extracted and analyzed with the ISE. When the same dry leaf tissue extracts were analyzed with the Cardy meter and the ISE, the results related well (r ² = 0.96). As a result of tissue processing and/or instrument differences, Cardy meter analysis of sap expressed from whole leaves was not comparable to ISE or colorimetric analyses of dry leaf tissue extracts for leafy green vegetables.

The use of polyethylene (PE) mulch causes environmental pollution where incomplete removal leaves fragments susceptible to escape to ecosystems, such as the ocean, where they can cause ecological harm. PE mulch is generally nonrecyclable due to contamination with soil and crop debris after use, leaving growers with few end-of-life options for used PE mulch. Research studies have shown that soil-biodegradable plastic mulch (BDM) is comparable to PE mulch in terms of performance, soil health, and overall economics and is preferred from an environmental perspective, but the adoption of BDM by producers is still low. Previous research has shown that the primary barriers to BDM adoption are insufficient knowledge about BDM, high purchase cost, and unpredictable breakdown of BDM in the soil. The high purchase cost of BDM compared with PE mulch is offset by the costs for PE mulch removal, transport, and disposal fees. This project was conducted to develop BDM training materials, to educate and assess BDM knowledge gained by extension personnel and other agricultural professionals through trainings and webinars, and to educate producers about BDM through hands-on experience. Thirty-six research and extension publication outputs from two previous US Department of Agriculture Specialty Crop Research Initiative BDM projects were reviewed and transcribed into 45 new extension publications that included 11 slide presentations, 5 lecture slides, 10 fact sheets, and 3 videos. All the training materials are posted on a public university website. Professional development trainings were conducted at local, regional, national, and international levels to provide agricultural professionals the current, science-based information on BDM and resources for information. Survey results showed that at a local level, the greatest change of knowledge among participants was observed for “BDM use in organic production” (60%), and the lowest reported change of knowledge was observed for “limitations to PE mulch disposal” (19%). At a regional level, out of 58 participants, 23% to 35% of participants learned “a lot” and 35% to 51% learned “some new information” regarding BDM from the webinar. At the national level, out of 30 participants, 48% responded that they learned “a lot” and another 48% learned “some new information” on BDM from the training. Growers were trained about BDM via field days and on-farm demonstrations where five strawberry (Fragaria ×ananassa) growers volunteered to participate in BDM trials. The participant growers observed no difference in weed control and fruit yield between the PE mulch and the BDM. Growers expressed concerns about slow biodegradation of BDM after soil incorporation, potential impacts on soil biological activity, food safety concerns with BDM fragments and that BDM is not currently permitted for use in organic production.

Mechanical hedging was evaluated at Washington State University Northwestern Washington Research and Extension Center, Mount Vernon, WA, in 2019 and 2020 on eight cider apple (Malus ×domestica) cultivars with four bearing habits: tip—Golden Russet, Harrison; spur—Brown Snout, Cap of Liberty; semispur—Tom Putt, Campfield; and crab—Puget Spice, Hewe’s Virginia Crab. Trees were planted on ‘Geneva 935’ (Malus hybrid) rootstock in one replicate block in 2014 and the second replicate block in 2016 and the central leader of all trees was headed in 2017 to equalize tree size and stage of development. Summer hedging was carried out on all cultivars on 16 July in 2019 and 7 July in 2020. The response of different cultivars was evaluated both years by measuring canopy area removed, shoot biomass removed, and fruit removed, and the amount of time to hedge was measured. Additionally, fruit diameter and fruit yield per tree were measured at harvest both years, and fruit weight was measured at harvest only in 2020. The hedger traveled at an average speed of 1.32 mph; it took 6 seconds on average to hedge both sides of one tree when in-row spacing was 6 ft and took 1.25 minutes to maneuver around the end of a row. The estimated time to hedge 1 acre was 1.45 hours when the hedger traveled at 116 ft/min and the orchard had 10 rows spaced 12 ft apart. Biomass removed on an area and weight basis was less in 2020 than in 2019, whereas yield per tree was 2.6 times greater in 2020 than 2019, and cultivars within a bearing habit differed in these responses to hedging both years. Fruit damaged by the hedger was assessed but observed to be negligible for all cultivars. Yield per tree was negatively correlated with fruit diameter (P < 0.001) and positively correlated with the number of fruit removed per tree (P < 0.025). Further research is needed to assess the long-term effects of hedging on biomass removal, yield, and biennialism to determine whether summer mechanical hedging is a cost-effective and suitable method for managing cider apple orchards.