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Vermicomposting of pig manure is a waste management approach that has been shown to be economically and technologically feasible and yields a value-added end product, vermicompost (VC), that contains plant-available nutrients. The objective of this study was to determine if conventional nursery crop inputs could be replaced by commercially available VC (derived from pig manure) for production of Hibiscus moscheutos ‘Luna Blush’ L. (hibiscus). Hibiscus was grown in 3.8-L containers containing pine bark amended with 11% sand (by vol.), 1.8 kg·m⁻³ dolomitic limestone, and 0.9 kg·m⁻³ micronutrient package (PBS) or pine bark amended with 20% VC (by vol.) (20VC). Plants were topdressed with one of three controlled-release fertilizers (CRF) containing only nitrogen (N); N and potassium (K); or N, phosphorus (P), and K. The four treatments included PBS with 17–6–12 (PBS + NPK), 20VC with 17–6–12 (20VC + NPK), 20VC with 17–0–12 (20VC + NK), and 20VC with 17–0–0 (20VC + N). The PBS + NPK treatment, which was supplied with conventional nursery crop nutrient inputs (limestone, sulfated micronutrients, and CRF containing NPK), served as the control treatment to represent the industry standard. All treatments were irrigated to maintain a leaching fraction (LF = volume leached ÷ volume applied) of 0.24. Daily inorganic nitrogen (IN-N) and dissolved reactive phosphorus (DRP) effluent contents were determined. Plants were harvested at 35 and 56 d after potting (DAP). Total plant nutrient contents of P, calcium (Ca), magnesium (Mg), and sulfur (S), iron (Fe), manganese, zinc (Zn), copper (Cu), and boron (B) were equivalent or greater for all three 20VC treatments compared with PBS + NPK. However, total plant K content of 20VC + N was less than 20VC + NPK, 20VC + NK, and PBS + NPK. Regardless of lower K content in the 20VC + N treatment, all three 20VC treatments had equivalent total plant dry weight and number of flowers. Furthermore, all three 20VC treatments averaged 58% and 40% greater plant dry weight than PBS + NPK at 35 and 56 DAP, respectively, and 93% more flowers than PBS + NPK at 56 DAP. All three 20VC treatments had similar IN-N and DRP effluent contents. However, the three 20VC treatments averaged 4.3× more IN-N effluent content and 59× DRP effluent content than PBS + NPK. The nutrient use efficiencies for all treatments were similar, in which nitrogen use efficiency ranged from 9% to 15% and phosphorus use efficiency ranged from 7% to 12%. In conclusion, this source of VC provided equivalent or greater P, Ca, Mg, S, Fe, Zn, and Cu but less K for plant uptake compared with the industry standard (control) treatment and produced larger plants with more flowers than the control. This suggests that dolomitic lime, sulfated micronutrients, and P can be eliminated as substrate additives.

The physical and chemical properties of pine bark yield low water and nutrient efficiency; consequently, an engineered substrate altering the substrate properties may allow greater water and nutrient retention. Past research has focused on controlling the quantity and rate of water and nutrient inputs. In this study, pine bark was amended at 8% (by volume) with a Georgiana palygorksite-bentonite blended industrial mineral aggregate with a particle size of 850 μm-4.75 mm or 300 μm-710 μm to improve water and nutrient efficiency. Each particle size was pretreated at temperatures of ≈140 °C (pasteurized) or ≈390 °C (calcined). The study was a 2 (particle size) × 2 (heat pretreatment) factorial in a randomized complete-block design with four replications. The control was a pine bark substrate amended with 11% sand (by volume). Containers (14 L) were topdressed with 17–5–12 controlled release fertilizer. A 0.2 leaching fraction was maintained by biweekly monitoring container influent from spray stakes and effluent volume measured daily. An aliquot of the daily collected effluent was analyzed for phosphorus (P). After 112 days, tops and roots were harvested, dried, and weighed for dry weight comparisons. Compared to pine bark amended with sand the 300 μm-710 μm particle size mineral decreased mean daily water application by ≈0.4 L/day per container. The calcined mineral reduced P leaching by ≈10 mg of P per container or 60% over the course of the study compared to pine bark: sand. Top and root dry weights were unaffected. These results suggest 300 μm–710 μm calcined mineral provided the most significant decreases in water use and P leaching while growing an equivalent plant.

Production of containerized nursery crops requires high inputs of water and mineral nutrients to maximize plant growth to produce a salable plant quickly. However, input efficiencies remain below 50% resulting in major quantities of water and nutrients leached. This study was conducted to determine if production factors could be altered to increase water and phosphorus uptake efficiency (PUE) without sacrificing plant growth. The effects of a pine bark substrate amendment (clay or sand) and a 50% reduction in both P application rate (1.0 g or 0.5 g) and leaching fraction (LF = effluent ÷ influent) (0.1 or 0.2) were investigated. Containerized Skogholm cotoneaster (Cotoneaster dammeri Schnied. ‘Skogholm’) was grown on gravel floor effluent collection plots that allowed for calculation of water and nutrient budgets. Pine bark amended with 11% (by vol.) Georgiana 0.25 to 0.85 mm calcined palygorksite-bentonite mineral aggregate (clay) increased available water 4% when compared with pine bark amended with 11% (by volume) coarse sand. Decreasing LF from 0.2 to 0.1 reduced cumulative container influent 25% and effluent volume 64%, whereas total plant dry weight was unaffected by LF. Reduction of target LF from 0.2 to 0.1 reduced dissolved reactive P concentration and content by 8% and 64%, respectively. In a sand-amended substrate, total plant dry weight decreased 16% when 1.0× P rate was reduced to 0.5× P, whereas total plant dry weight was unaffected by rate of P when pine bark was amended with clay. Plant content of all macronutrients, with the exception of N, increased when pine bark was amended with clay versus sand. Reducing P rate from 1.0× to 0.5× increased PUE 54% or 11% in a clay or sand-amended substrate, respectively. Amending pine bark with 11% (by volume) 0.25 to 0.85 mm calcined palygorksite-bentonite mineral aggregate produced an equivalent plant with half the P inputs and a 0.1 LF, which reduced water use 25% and P effluent losses 42% when compared with an industry representative substrate [8 pine bark : 1 sand (11% by volume)].

Nonpoint source effluent containing nitrate N (NO₃-N) and phosphorus (P) from containerized nursery production has garnered local, regional, and national concern. Industrial minerals have long been used as absorbents, agrochemical carriers, and barriers to retain heavy metals. Our objective was to determine the effects of a palygorskite–bentonite industrial mineral aggregate on the physical and chemical properties of a soilless substrate and the resulting impact on water and nutrient efficiency. The mineral aggregate had two particle size ranges (0.25 to 0.85 mm or 0.85 to 4.75 mm) in combination with two temperature pretreatments [low volatile material (LVM) or regular volatile material (RVM)]. A representative substrate (8 pine bark:1 coarse sand) of the southeastern United States nursery industry was also included in the study as a control. Cotoneaster dammeri C.K. Schneid. ‘Skogholm’ was grown in all substrates on collection pads that allowed for the quantification of daily influent and effluent volumes to calculate cumulative NO₃-N, ammonium N (NH₄-N), and dissolved reactive phosphorus (DRP) loss for 112 days. There was a 13% to 15% decrease in daily water application volume with no effect on Skogholm cotoneaster growth, which equated into a savings of 22 to 26 L per 14-L container in mineral aggregate-amended substrates compared with a sand-amended substrate (control). Mineral aggregate-amended substrates decreased NH₄-N and DRP effluent 39% and 34%, respectively, compared with the control. In addition, LVM and particle size 0.25 to 0.85 mm reduced effluent DRP compared with the 0.85 to 4.75-mm RVM aggregate. Plant dry weight was unaffected by any of the treatments. Substantial nutrient content reduction in effluent and reductions in water application were achieved with amending pine bark with 0.25 to 0.85 mm LVM industrial mineral aggregate. A 0.25 to 0.85-mm LVM industrial mineral aggregate pine bark-amended substrate reduced effluent DRP and NH₄-N greater than 40% and reduced water application 15% or 26 L when compared with the industry representative substrate.

Catawba rhododendron (Rhododendron catawbiense Michx.) seedlings of two provenances, Johnston County, N.C. (35°45′N, 78°12′W, elevation = 67 m), and Yancey County, N.C. (35°45′N, 82°16′W, elevation = 1954 m), were grown in controlled-environment chambers for 18 weeks with days at 18, 22, 26, or 30C in factorial combination with nights at 14, 18, 22, or 26C. Seedlings of the higher-elevation provenance generally exhibited higher net leaf photosynthetic rates (P_N)s than those from the lower elevation at all temperature combinations. Thus, it appears seedlings of the high-elevation provenance possess greater relative thermotolerance, expressed as net photosynthesis, than the low-elevation provenance. Eighty-seven days after initiation (DAI) of the experiment, P_N showed a quadratic response to increasing day temperature, with the maximum occurring at 22C, whereas P_N decreased linearly with increasing night temperature. At 122 DAI, P_N increased linearly with increasing day temperature with nights at 22 and 26C. Highest P_Ns were at 30/22C and 26/22C. Carbohydrate export increased with increasing day temperature, whereas the response to night temperature was minimal. High levels of nonstructural carbohydrates occurred at thermoperiods (22/22C and 26/22C) that optimize seedling growth. However, definitive trends relating seedling growth to P_Ns, leaf carbohydrate levels, or to the amount of carbohydrate exported from the leaves were difficult to generalize due to numerous day × night interactions.

Seedlings of flame azalea [Rhododendron calendulaceum (Michx.) Torr] were grown for 12 weeks under long-day conditions with days at 18, 22, 26, or 30C for 9 hours in factorial combination with nights at 14, 18, 22, or 26C for 15 hours. Total plant dry weight, top dry weight, leaf area, and dry weights of leaves, stems, and roots were influenced by day and night temperatures and their interactions. Dry matter production was lowest with nights at 14C. Root, leaf, top, and total dry weights were maximized with days at 26C in combination with nights at 18 to 26C. Stem dry weight was maximized with days at 26 to 30C and nights at 22C. Leaf area was largest with days at 18 and 26C in combination with nights at 18 or 26C. Within the optimal, day/night temperature range of 26 C/18-26C for total plant dry weight, there was no evidence that alternating temperatures enhanced growth. Shoot: root ratios (top dry weight: root dry weight) were highest with days at 18 and 30C. Leaf area ratio (total leaf area: total plant dry weight) was highest and specific leaf area (total leaf area: leaf dry weight) was largest when days and nights were at 18C and were lower at higher temperatures. Regardless of day/night temperature, leaf weight ratio (leaf dry weight: total plant dry weight) was higher than either the stem weight ratio (stem dry weight: total plant dry weight) or root weight ratio (root dry weight: total plant dry weight). Net leaf photosynthetic rate increased with day temperatures up to 30C.

Abstract

Seven vegetation management programs ranging from 100% cover of grass-dominated vegetation to bare soil were created on opposing north and south aspects. After 3 years, fraser fir [Abies fraseri (Pursh) Poir.] survival had decreased when grown in bare soil, compared to survival in the other management programs. Norway spruce [Picea abies (L.) Karst.] survival was not affected by the management programs. Maximum stem diameter and root growth of Norway spruce were obtained with a bare row regardless of the interrow vegetation. Root growth in fraser fir was similar to spruce, but bare soil was required for maximum stem diameter growth. Height growth in both species was affected little by treatment. Stem diameter and root growth were negatively correlated with above-ground herbaceous biomass in the row. Forbs interfered less than grasses with fraser fir and Norway spruce diameter growth. Norway spruce growth was not affected by aspect, but fraser fir was larger (height and stem diameter) on the south aspect when grown in bare soil.

Uniconazole was applied as a foliar spray at 0, 90, 130, 170, or 210 ppm to rooted stem cuttings of `Spectabilis' forsythia (Forsythia xintermedia Zab.) potted in calcined clay. Uniconazole resulted in higher total leaf chlorophyll (chlorophyll + chlorophyll,) concentration and a decreased ratio of chlorophyll a: b. Stomata1 density of the most recently matured leaves increased linearly with increasing uniconazole concentration 40, 60, and 100 days after treatment (DAT). The number of stomata per leaf (stomata1 index) increased linearly with increasing concentration of uniconazole throughout the initial 100 DAT. Uniconazole suppressed stomata1 length at all sampling dates and the level of suppression increased with increasing concentration of uniconazole from 20 to 100 DAT. Stomata1 width was suppressed by uniconazole at 40 DAT. Leaves developed after uniconazole application had higher levels of net photosynthesis when measured 55, 77, and 365 DAT. Stomata1 conductance for uniconazole-treated plants was higher compared to nontreated control (0 mg·liter^-1) plants when measured 49, 55, 77, and 365 DAT. Initiation of secondary xylem for stem tissues of uniconazole-treated plants was suppressed and expansion of xylem vessel length and width was less. Secondary phloem tissues of stems from uniconazole-treated plants contained larger numbers of phloem fibers having smaller cross sectional areas than phloem fibers of controls. Chemical name used: (E)-1-(p-Chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-01 (uniconazole).

Seedlings of mountain laurel (Kalmia latifolia L.) were grown for 16 weeks under long-day conditions with days at 18, 22, 26, or 30C for 9 hours in factorial combination with nights at 14, 18, 22, or 26C for 15 hours. Total plant dry weight, top dry weight, and dry weights of leaves, stems, and roots were influenced by day and night temperatures. The night optimum for all dry weight categories was 22C. Dry matter production was lowest with nights at 14C. Total plant dry weight and dry weights of tops, leaves, and stems were maximized with days at 26C, but for roots the optimum was 22C. Dry weight accumulation was lower with days at 18 or 30C. Responses of leaf area were similar to that of total plant dry weight, with optimum days and nights at 26 and 22C, respectively. Within the optimal day/night temperature range of 22-26/22C for dry weights, there was no evidence that alternating temperatures enhanced growth. Shoot: root ratios (top dry weight: root dry weight) increased with day temperatures up to 30C and were highest with nights at 14 or 26C. Leaf weight ratio (leaf dry weight: total plant dry weight) decreased with increasing night temperature, and increased curvilinearly in response to day temperature with the minimum at 26C. Stem weight ratio (stem dry weight: total plant dry weight) increased with increasing day or night temperature. Root weight ratio (root dry weight: total plant dry weight) was highest with nights at 18 or 22C and decreased with days >22C. Net leaf photosynthetic rate was maximized with days at 26C.

Many research studies have evaluated potential organic and mineral container substrate components for use in commercial potting substrates. Most studies report results of plant growth over a single production season and only a few include physical properties of the substrates tested. Furthermore, substrates containing predominantly organic components decompose during crop production cycles producing changes in air and water ratios. In the commercial nursery industry, crops frequently remain in containers for longer periods than one growing season (18 to 24 months). Changes in air and water retention characteristics over extended periods can have significant effect on the health and vigor of crops held in containers for 1 year or more. Decomposition of organic components can create an overabundance of small particles that hold excessive amounts of water, thus creating limited air porosity. Mineral aggregates such as perlite, pumice, coarse sand, and calcined clays do not decompose, or breakdown slowly, when used in potting substrates. Blending aggregates with organic components can decrease changes in physical properties over time by dilution of organic components and preserving large pore spaces, thus helping to maintain structural integrity. Research is needed to evaluate changes in container substrates from initial physical properties to changes in air and water characteristics after a production cycle.