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  • Author or Editor: J.J. Luby x
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Three classes of crosses using four genotypes of V. riparia (wild Riverbank grape) as maternal parents were evaluated for evidence of reproductive expression of genetic incongruity. The classes were: I V. riparia x V. vinifera cultivars (European domesticated grape); II V. riparia x French Hybrids (complex interspecific hybrids); III V. riparia x V. riparia. Percent fruit set and seeds per berry were recorded for two years. If incongruity is a factor in interspecific grape crosses, then the values for these traits would be expected to be lower in classes I and II than in class III. Analysis of variance indicated significant differences for some half-sib families. Fruit and seed set were lower in classes I and II than in class III, suggesting that incongruity is operative in wide grape crosses. In the process of creating French hybrids, genomes of several species came together over generations of hybridization. In concert with selection for fertility, repeated interspecific genomic exposure would be expected to have ameliorated the effects of initial incongruity between American species and V. vinifera, increasing their value as genetic bridges in breeding programs.

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As part of our hardy strawberry (Fragaria ×ananassa) breeding program, winter hardiness of 15 strawberry cultivars was evaluated in the field after Winter 2005–2006 and a test Winter 2006–2007 with no snow cover at Grand Rapids, MN. After the snow-covered Winter 2005–2006, plant stand (percent leaf coverage for the designated area for each plot) increased for all cultivars in the mulched treatment and some cultivars in the unmulched treatment with slight decreases only for several cultivars in the unmulched treatment. However, after Winter 2006–2007, the plant stands of all cultivars drastically decreased in both mulched and unmulched treatments. ‘Clancy’, ‘Evangeline’, and ‘L'Amour’ were the three most sensitive cultivars among the 15 cultivars tested. ‘Kent’, ‘Mesabi™’, ‘Cavendish’, and ‘Brunswick’ were the highest yielding cultivars for both 2006 and 2007 in the mulched treatment. In the unmulched treatment, ‘Brunswick’, ‘Mesabi™ ’, ‘Cavendish’, ‘Sable’, and ‘Kent’ were the top yielding cultivars after Winter 2006–2007. During Winter 2005–2006, with 20 to 30 cm snow cover throughout the season, the 5- and 10-cm soil temperatures remained constant at ≈30 to 31.5 °F in both mulched and unmulched treatments. In contrast, during Winter 2006–2007, there were 16 and 24 days (consecutive) in February below 18 °F at 5-cm soil depths for mulched and unmulched treatments, respectively, which probably led to the severe winter damage. Although straw mulch afforded the plants some protection, snow cover is critical to the survival of strawberries in northern Minnesota and other areas with similar weather conditions.

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Zanthoxylum americanum is a common understory species in the northern forests of Minnesota and surrounding regions. It has potential economic importance for its citrus fragrance, pharmacological or insecticidal properties, and produces peppercorns similar to those of the related Zanthoxylum species. Zanthoxylum americanum is a dioecious species but has been reported to have aberrant flowers with autonomous apomixis instead of other potential reproductive barriers. The reproductive biology of Zanthoxylum americanum was investigated in two native Minnesota populations. Determinations of male fertility, whether autonomous apomixis was the predominant floral reproductive mechanism, the presence of seedless fruit (parthenocarpy/stenospermocarpy), and the occurrence of hermaphrodism were made over 2 years. Sex ratios (female:male plants) within each population differed. The mean pollen stainability was 95.8% ± 0.3% (fresh) and 78.6% ± 1.1% (stored 18 months). Parthenocarpy did not occur in either population. Autonomous apomixis was not the primary floral reproductive mechanism. Stenospermocarpy (seedlessness) occurred in 13% of the female fruit clusters. Although commonly described as being dioecious, two additional reproductive strategies were identified: 1) plants with functional protandrous flowers with rudimentary pistils and 2) hermaphroditic flowers with fully functional pistils (protogynous) and anthers. As many as 10% to 30% of the male plants bore at least one fruit/plant each year. One clonal stand had both hermaphroditic and functionally staminate flowers on the same plant. Two evolutionary pathways to dioecy in Z. americanum are proposed.

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Pollination of the half-high blueberry (Vaccinium corymbosum L./V. anugustifolium Ait.) cultivars St. Cloud, Northsky, Northcountry, and Northblue with self, outcross, and outcross/self pollen mixtures suggests that outcross fertilization maximizes percent fruit set, berry weight, seeds per berry, and seeds per pollination while minimizing days to harvest. Based on these results, mixed plantings of at least two blueberry cultivars are recommended for these cultivars. Fruit and seed set were negatively associated with increased percentages of self pollen in outcross/self pollen mixtures. These responses were linear for `Northblue' due to a tendency to parthenocarpy, and nonlinear for `St. Cloud', `Northsky', and `Northcountry', due to low fruit set following self-pollination. These data indicate that post-fertilization abortion affected seed formation, which was, in turn, correlated positively with fruit set.

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Narrow-sense heritability and among-family and within-family variance components were estimated for antioxidant activity (AA), total phenolic content (TPH), and anthocyanin content (ACY) in blueberry (Vaccinium L. sp.) fruit. AA, TPH, and ACY were determined in the parents and in 10 offspring from each of 20 random crosses for each of 2 years at Becker, Minn. Offspring-midparent regression analysis provided combined-year heritability estimates of 0.43 ± 0.09 (P ≤ 0.0001) for AA, 0.46 ± 0.11 (P ≤ 0.0001) for TPH, and 0.56 ± 0.10 (P ≤ 0.0001) for ACY. Analyses of variance delineated variation among and within families for AA, TPH, and ACY (P ≤ 0.001). Year-to-year variation in the means for all offspring genotypes was not significant for AA or TPH, but there were changes in rank between years for families and for offspring within families for these traits. Year-to-year variation in the mean for all offspring genotypes was significant for ACY, but rank changes were observed only among offspring within families, not among families. In total, 18 of 200 offspring from 7 of the 20 crosses were transgressive segregants for AA, exceeding the higher parent of the cross by at least two sds. Estimates of variance components showed that variation among families accounted for 24% to 27% of total variance for the three traits. However, variation within families was greater than that among families, accounting for 38% to 56% of total variance for the three traits. These results suggest that increasing antioxidant activity in blueberry through breeding is feasible, and that the breeding strategies utilized should exploit the large within-family variation that exists.

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Variation in antioxidant activity (AA), total phenolic content (TPH), and total anthocyanin content (ACY) was examined in 1998 and 1999 in fruit of 52 (49 blue-fruited and 3 pink-fruited) genotypes from a blueberry breeding population. The species ancestry included Vaccinium corymbosum L. (northern highbush blueberry), V. angustifolium Ait. (lowbush blueberry), V. constablaei Gray (mountain highbush blueberry), V. ashei Reade (rabbiteye blueberry), and V. myrtilloides Michx. (lowbush blueberry). Using a methyl linoleate oxidation assay (MeLO) on acidified methanolic extracts of the berries, a 5-fold variation was found in AA in 1998 and a 3-fold variation in 1999 among the blue-fruited genotypes. Analyses of variance (ANOVA) revealed variation among genotypes (P < 0.0001) in single and combined years, regardless of inclusion of pink-fruited selections and adjustment for berry size. While mean AA of all genotypes did not change between the 2 years, ranking of some genotypes for AA changed significantly between 1998 and 1999. Of the 10 genotypes that demonstrated the highest AA in 1998, four were among the 10 genotypes that demonstrated highest AA in 1999. Similarly, of the 15 genotypes with the highest AA, 10 were the same both years. As with AA, mean TPH of all genotypes did not change between years and ANOVA demonstrated genotypic variation regardless of adjustment for berry size/weight or exclusion of pink-fruited selections. Changes in genotype rank occurred between years. The difference in TPH between lowest- and highest-ranking blue-fruited genotypes was ≈2.6-fold in both 1998 and 1999. Seven of the 10 highest-ranking genotypes were the same both years and TPH correlated with AA (r = 0.92, P < 0.01) on a genotype mean basis for combined years. ACY correlated less well with AA (r = 0.73, P < 0.01 for combined years). When genotypes were categorized into six groups according to species ancestry, V. myrtilloides and V. constablaei × V. ashei crosses ranked highest and second highest, respectively, for AA in both years. The groups comprised of V. corymbosum genotypes, V. angustifolium genotypes, and those with both V. corymbosum and V. angustifolium in their lineage were indistinguishable from each other. Samples from some of the genotypes were analyzed for oxygen radical absorbance capacity and ferric-reducing antioxidant power, and these aqueous-based antioxidant assays correlated well with the lipid emulsion-based MeLO (all r ≥ 0.90, P < 0.01). The three antioxidant assays may be equally useful for screening in a blueberry breeding program and the choice of assay may depend on the goal of the program and the resources available.

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Restriction fragment-length polymorphisms (RFLPs) of chloroplast DNA (cpDNA) were used to study phylogenetic relationships among twenty-six Fragaria taxa and two closely related species, Potentilla fruticosa L. and Duchesnea indica (Andrews) Focke. Sixteen restriction enzymes and probes of the entire Nicotiana tabacum L. chloroplast genome revealed a very low level of variation among the Fragaria taxa, limiting phylogenetic resolution. However, Fragaria appears to be more closely related to Potentilla than Duchesnea. The diploid taxa, F. iinumae Makino, F. nilgerrensis Schlect. and F. vesca L. were the most divergent Fragaria taxa and F. iinumae appears to be the most ancestral taxon. Little variation was revealed within the economically important octoploid group of taxa, which gave rise to the cultivated strawberry, and no progenitor taxa to the octoploid group could be identified. The lack of variation in the chloroplast genome suggests that these Fragaria species may be of relatively recent evolutionary origin.

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In the current global market, the development of novel products is important for staying competitive. The development of horticultural products often manifests as new cultivars. Gauging consumer interest is an important step in cultivar development because it is a resource-intensive process. The present study used an experimental auction to measure consumers’ willingness to pay (WTP) for novel fruit referred to as kiwiberry (Actinidia arguta and Actinidia kolomikta) and explore consumer segmentation for their preferences. The mean WTP for 6-ounce packages of kiwiberries ranged from $1.63 to $2.19, depending on species and cultivar. Four groups of consumers were identified in relation to their WTP for kiwiberries. Using survey data, socio-demographic variables such as age, education, and neophobic attitudes were significantly different between the mixed kiwiberry price premium and kiwiberry discounting groups. These findings suggest that consumers with variety-seeking tendencies have a WTP for kiwiberries that is comparable to that for other berries. Marketing strategies for these groups are proposed.

Open Access

Progenies from crosses among eight highbush (Vaccinium corymbosum L.), lowbush (V. angustifolium Ait.), and V. corymbosum/V. angustifolium hybrid-derivative parents were evaluated in vitro at low (5.0) and high (6.0) pH for vitality, height, and dry weight. Succinic acid and 2[N- morpholino]ethanesulfonic acid (Mes) effectively maintained pH in the medium and rhizosphere. The pH regime did not affect percent radicle emergence from seed or survival; however, percent seed germination was slightly lower at high pH. The parental general combining ability (GCA), reciprocal and maternal, but not the specific combining ability (SCA) variance components were significant for plant vitality, height, and dry weight. The GCA variance components were six to 26 times larger than the SCA variance components for the plant growth traits. Variation due to pH regime was significant for vitality and dry weight but not for plant height. The progenies of parents with high percent lowbush ancestry were taller at both pH levels than those with less such ancestry. Little variation was apparent for higher pH tolerance as measured by dry weight; however, the GCA effects suggested that the progenies of some parents performed better than others at high pH. Vaccinium angustifolium parents differed in the extent to which tolerance to high pH was transmitted. In vitro screening in concert with a traditional breeding program should be effective in improving blueberry tolerance to higher pH.

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